Journal: Nature Communications

Article Title: Oxytocin stimulates hippocampal neurogenesis via oxytocin receptor expressed in CA3 pyramidal neurons

doi: 10.1038/s41467-017-00675-5

Figure Lengend Snippet: Conditional deletion of Oxtr in the excitatory neurons of mouse hippocampus. a Top panel , a representative immunofluorescence image of OXTR ( green ) expression in the mouse hippocampus. Bottom panels , augmented figures showing CaMKIIα ( red ) and OXTR ( green ) double-labeling results in rectangle area . Scale bars : top , 200 μm; bottom , 50 μm. b PCR screening of tail-derived genomic DNA for selection of Oxtr −/− mice. c Quantitative real-time PCR of Oxtr mRNA in the CA2, CA3, hilus, and hypothalamus ( HT ) regions from WT ( Oxtr f/f ) and Oxtr −/− mice ( n = 4 mice for each group; * P < 0.05, unpaired two-tailed Student’s t test). d , e FISH images showing the expression of Oxtr mRNA in the CA2 d and CA3 regions e of WT and Oxtr −/− mice (counterstained with DAPI, blue ). Scale bar , 50 μm. Data was replicated in four mice

Article Snippet: After blocking, the sections were incubated in the primary antibodies against nestin (1:500; Millipore, MAB353), Ki67 (1:500; Abcam, ab15580), DCX (1:500; Cell Signaling Technology, #4604), NeuN (1:1000; Millipore, ABN78), calretinin (1:500; Millipore, MAB1568), parvalbumin (1:500; Swant, PV235), calbindin (1:500; Millipore, AB1778), CaMKIIα (1:500; Novas Biologicals, NB100-81830), or GAD67 (1:500, Millipore, MAB5406).

Techniques: Immunofluorescence, Expressing, Labeling, Derivative Assay, Selection, Real-time Polymerase Chain Reaction, Two Tailed Test