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KEY RESOURCES TABLE

Journal: Current biology : CB

Article Title: Tyrosine phosphorylation of the myosin regulatory light chain controls non-muscle myosin II assembly and function in migrating cells

doi: 10.1016/j.cub.2020.04.057

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies Antibodies against the following antigens were used: MHCII-A and -B (#909801 and #909901, respectively) from Biolegend; Myo18A (#NB100-79839) from Novus Biologicals; myosin RLC pS19 (#3675) and RLC pT18/S19 (#3674) from Cell Signaling Technologies; RLC pS1 (#MP3461) from ECM Biosciences; total RLC (indicated as RLC in the figures, # 600-401-938) from Rockland; EGFR (#AHR5072), from Thermo Fisher Scientific; RLC (indicated as MY-21 in the figures, # M4401), vinculin (#V9131), α-tubulin (#T9026), FLAG (#F3165) and vimentin (#V6630) from Merck; and GFP (#9996) from Santa Cruz Biotechnology.

Techniques: Magnetic Beads, Virus, Recombinant, Membrane, Protease Inhibitor, Plasmid Preparation, Software, Mass Spectrometry, Chemotaxis Assay, Migration, Pore Size

KEY RESOURCES TABLE

Journal: Current biology : CB

Article Title: Tyrosine phosphorylation of the myosin regulatory light chain controls non-muscle myosin II assembly and function in migrating cells

doi: 10.1016/j.cub.2020.04.057

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-Myo18A , Novus Biologicals , Cat#NB100–79839.

Techniques: Magnetic Beads, Virus, Recombinant, Membrane, Protease Inhibitor, Plasmid Preparation, Software, Mass Spectrometry, Chemotaxis Assay, Migration, Pore Size