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antibodies against cd24  (Novus Biologicals)
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Novus Biologicals antibodies against cd24
CSCs are identified within the chordoma tumor microenvironment. Representative photomicrographs from one chordoma of merged and single-color immunofluorescence images assessing the presence of CSCs with a validated panel of five biomarkers, PD-L1 (yellow, Opal 570), CD15 (red, Opal 690), ALDH (orange, Opal 620), <t>CD24</t> (green, Opal 520), and CK (white, Opal 780). Multispectral immunofluorescence images are counterstained with DAPI. Co-localization of CD15, ALDH, and CD24 identified CSCs. Co-localization of these markers with PD-L1 identified PD-L1 + CSCs. CD15 + CD24 + ALDH + CK + CSCs comprised 1.39% of all tumor cells. CK, cytokeratin; DAPI, 4′,6-diamidino-2- phenylindole; CSC, cancer stem cells.
Antibodies Against Cd24, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against cd24 - by Bioz Stars, 2026-04
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CSCs are identified within the chordoma tumor microenvironment. Representative photomicrographs from one chordoma of merged and single-color immunofluorescence images assessing the presence of CSCs with a validated panel of five biomarkers, PD-L1 (yellow, Opal 570), CD15 (red, Opal 690), ALDH (orange, Opal 620), CD24 (green, Opal 520), and CK (white, Opal 780). Multispectral immunofluorescence images are counterstained with DAPI. Co-localization of CD15, ALDH, and CD24 identified CSCs. Co-localization of these markers with PD-L1 identified PD-L1 + CSCs. CD15 + CD24 + ALDH + CK + CSCs comprised 1.39% of all tumor cells. CK, cytokeratin; DAPI, 4′,6-diamidino-2- phenylindole; CSC, cancer stem cells.

Journal: Frontiers in Oncology

Article Title: Chordoma cancer stem cell subpopulation characterization may guide targeted immunotherapy approaches to reduce disease recurrence

doi: 10.3389/fonc.2024.1376622

Figure Lengend Snippet: CSCs are identified within the chordoma tumor microenvironment. Representative photomicrographs from one chordoma of merged and single-color immunofluorescence images assessing the presence of CSCs with a validated panel of five biomarkers, PD-L1 (yellow, Opal 570), CD15 (red, Opal 690), ALDH (orange, Opal 620), CD24 (green, Opal 520), and CK (white, Opal 780). Multispectral immunofluorescence images are counterstained with DAPI. Co-localization of CD15, ALDH, and CD24 identified CSCs. Co-localization of these markers with PD-L1 identified PD-L1 + CSCs. CD15 + CD24 + ALDH + CK + CSCs comprised 1.39% of all tumor cells. CK, cytokeratin; DAPI, 4′,6-diamidino-2- phenylindole; CSC, cancer stem cells.

Article Snippet: The CSC panel was comprised of antibodies against CD24 (Novus Biologicals [ML5], #NB100-77903; 1:1000), ALDH1 (Abcam [EP1933Y], #ab52492; 1:400), PD-L1 (Cell Signaling [E1L3N(R)], #13684S; 1:300), CD15 (BD Biosciences [HI98], #555400; 1:1500), and Cytokeratin (Santa Cruz [AE1/AE3], #sc-81714; 1:400) ( ).

Techniques: Immunofluorescence

CSC and PD-L1 percent positivity in chordoma cells.

Journal: Frontiers in Oncology

Article Title: Chordoma cancer stem cell subpopulation characterization may guide targeted immunotherapy approaches to reduce disease recurrence

doi: 10.3389/fonc.2024.1376622

Figure Lengend Snippet: CSC and PD-L1 percent positivity in chordoma cells.

Article Snippet: The CSC panel was comprised of antibodies against CD24 (Novus Biologicals [ML5], #NB100-77903; 1:1000), ALDH1 (Abcam [EP1933Y], #ab52492; 1:400), PD-L1 (Cell Signaling [E1L3N(R)], #13684S; 1:300), CD15 (BD Biosciences [HI98], #555400; 1:1500), and Cytokeratin (Santa Cruz [AE1/AE3], #sc-81714; 1:400) ( ).

Techniques:

(A) HALO® density heat map analyses suggest that CSCs are found within clusters in four representative chordoma tumors. Solid blue patches delineate tumor areas included in the analysis. Grouped yellow and green dots, marked using white arrows, denote CSC clusters. (B) CK + chordoma cells triple positive for cancer stem cell markers CD15, CD24, and ALDH are denoted CSCs. CSCs, cancer stem cells.

Journal: Frontiers in Oncology

Article Title: Chordoma cancer stem cell subpopulation characterization may guide targeted immunotherapy approaches to reduce disease recurrence

doi: 10.3389/fonc.2024.1376622

Figure Lengend Snippet: (A) HALO® density heat map analyses suggest that CSCs are found within clusters in four representative chordoma tumors. Solid blue patches delineate tumor areas included in the analysis. Grouped yellow and green dots, marked using white arrows, denote CSC clusters. (B) CK + chordoma cells triple positive for cancer stem cell markers CD15, CD24, and ALDH are denoted CSCs. CSCs, cancer stem cells.

Article Snippet: The CSC panel was comprised of antibodies against CD24 (Novus Biologicals [ML5], #NB100-77903; 1:1000), ALDH1 (Abcam [EP1933Y], #ab52492; 1:400), PD-L1 (Cell Signaling [E1L3N(R)], #13684S; 1:300), CD15 (BD Biosciences [HI98], #555400; 1:1500), and Cytokeratin (Santa Cruz [AE1/AE3], #sc-81714; 1:400) ( ).

Techniques: