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A. Representative example of <t>DR3</t> flow cytometry analysis. Dashed line: isotypic control; regular line: anti-DR3 signal. B. Time course analysis of DR3 expression in unstimulated and anti-IgM-stimulated CLL cells ( n = 8). C. Surface DR3 expression in unstimulated and anti-IgM-stimulated CLL cells ( n = 36). Data are expressed as DR3-expression median fluorescence intensity (MFI) divided by isotype-matched control (relative median fluorescence intensity = RMFI). Comparison between unstimulated and anti-IgM-stimulated CLL cells was performed by the two-sample Wilcoxon signed rank sum test. D. Comparison of DR3 expression between B cells from CLL samples ( n = 36) and age-matched healthy donors ( n = 10). Lines represent median values. Comparison was performed using the Mann-Whitney test. ns = not significant. E. Western blot analysis of cell lysates from purified CLL cells ( n = 4), in unstimulated and anti-IgM stimulated conditions. Level of DR3 induction after anti-IgM stimulation is reported as fold change. F. Representative example of DR3 immunofluorescence analysis in CLL lymph-node tissue sections ( n = 2). Panel A: pseudocolour image of DR3 (200×); Panel B: pseudocolour image of CD23 (200×); Panel C: merged pseudocolour image of CD23 (green), DR3 (red) and DNA (blu) (200×).
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A. Representative example of DR3 flow cytometry analysis. Dashed line: isotypic control; regular line: anti-DR3 signal. B. Time course analysis of DR3 expression in unstimulated and anti-IgM-stimulated CLL cells ( n = 8). C. Surface DR3 expression in unstimulated and anti-IgM-stimulated CLL cells ( n = 36). Data are expressed as DR3-expression median fluorescence intensity (MFI) divided by isotype-matched control (relative median fluorescence intensity = RMFI). Comparison between unstimulated and anti-IgM-stimulated CLL cells was performed by the two-sample Wilcoxon signed rank sum test. D. Comparison of DR3 expression between B cells from CLL samples ( n = 36) and age-matched healthy donors ( n = 10). Lines represent median values. Comparison was performed using the Mann-Whitney test. ns = not significant. E. Western blot analysis of cell lysates from purified CLL cells ( n = 4), in unstimulated and anti-IgM stimulated conditions. Level of DR3 induction after anti-IgM stimulation is reported as fold change. F. Representative example of DR3 immunofluorescence analysis in CLL lymph-node tissue sections ( n = 2). Panel A: pseudocolour image of DR3 (200×); Panel B: pseudocolour image of CD23 (200×); Panel C: merged pseudocolour image of CD23 (green), DR3 (red) and DNA (blu) (200×).

Journal: Oncotarget

Article Title: Expression and function of the TL1A/DR3 axis in chronic lymphocytic leukemia

doi:

Figure Lengend Snippet: A. Representative example of DR3 flow cytometry analysis. Dashed line: isotypic control; regular line: anti-DR3 signal. B. Time course analysis of DR3 expression in unstimulated and anti-IgM-stimulated CLL cells ( n = 8). C. Surface DR3 expression in unstimulated and anti-IgM-stimulated CLL cells ( n = 36). Data are expressed as DR3-expression median fluorescence intensity (MFI) divided by isotype-matched control (relative median fluorescence intensity = RMFI). Comparison between unstimulated and anti-IgM-stimulated CLL cells was performed by the two-sample Wilcoxon signed rank sum test. D. Comparison of DR3 expression between B cells from CLL samples ( n = 36) and age-matched healthy donors ( n = 10). Lines represent median values. Comparison was performed using the Mann-Whitney test. ns = not significant. E. Western blot analysis of cell lysates from purified CLL cells ( n = 4), in unstimulated and anti-IgM stimulated conditions. Level of DR3 induction after anti-IgM stimulation is reported as fold change. F. Representative example of DR3 immunofluorescence analysis in CLL lymph-node tissue sections ( n = 2). Panel A: pseudocolour image of DR3 (200×); Panel B: pseudocolour image of CD23 (200×); Panel C: merged pseudocolour image of CD23 (green), DR3 (red) and DNA (blu) (200×).

Article Snippet: Briefly, DR3 expression was detected using anti-DR3-biotinylated mAb (clone JD3, cat#NB100–77847, Novus Biologicals, Cambridge, England).

Techniques: Flow Cytometry, Control, Expressing, Fluorescence, Comparison, MANN-WHITNEY, Western Blot, Purification, Immunofluorescence

DR3 expression was associated with clinical staging according to Rai or Binet classification, or with standard prognostic markers: IGHV status, CD38 and ZAP-70 expression, and cytogenetic risk categories. Lines represent median values. Comparison was performed using the Mann-Whitney test and Kruskal-Wallis test. ns = not significant.

Journal: Oncotarget

Article Title: Expression and function of the TL1A/DR3 axis in chronic lymphocytic leukemia

doi:

Figure Lengend Snippet: DR3 expression was associated with clinical staging according to Rai or Binet classification, or with standard prognostic markers: IGHV status, CD38 and ZAP-70 expression, and cytogenetic risk categories. Lines represent median values. Comparison was performed using the Mann-Whitney test and Kruskal-Wallis test. ns = not significant.

Article Snippet: Briefly, DR3 expression was detected using anti-DR3-biotinylated mAb (clone JD3, cat#NB100–77847, Novus Biologicals, Cambridge, England).

Techniques: Expressing, Comparison, MANN-WHITNEY