Journal: European journal of medical genetics
Article Title: Altered peripheral blood leukocyte subpopulations, function, and gene expression in children with Down syndrome: implications for respiratory tract infection.
doi: 10.1016/j.ejmg.2024.104922
Figure Lengend Snippet: Fig. 1. Persons with DS circulate unique peripheral blood leukocytes and plasma cytokine profiles. A) Fluorescence-activated cell sorting (FACS) gating strategy for peripheral blood leukocyte phenotypic and functional analyses. LDG = low density granulocytes; AAM = alternatively activated macrophages. B) Representative FACS gating for (top left to right) total cells, singlets, CD19, CD15/CD14. Representative FACS gating from a typical individual and a person with DS for CD15+ LDG (lower left) and CD14+ AAM (lower right). In this individual with DS, there are more LDG and AAM than the control sample. C) As a percentage of CD14+ or CD15+
Article Snippet: Cells in whole blood were labeled using antibodies against CD11bAPC (550019; BD Biosciences), CD14-FITC (NB100 77759; Novus), CD15-PE (NB100 1831 PE; Novus), CD103-DyLight405 (NB1-9 7564V3; Novus), CD163 Alexa 405 (NBP2-36494AF405; Novus), CD 206 PECyanine 7 (25-2069-426; eBioscience), and CD19-APC-eFluor 780 (47–0198; eBioscience), per manufacturer’s protocol.
Techniques: Clinical Proteomics, Fluorescence, FACS, Functional Assay, Control