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histone h2ax [p ser139] antibody (3f2)  (Bio-Techne corporation)


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    Bio-Techne corporation histone h2ax [p ser139] antibody (3f2)
    Histone H2ax [P Ser139] Antibody (3f2), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/histone h2ax [p ser139] antibody (3f2)/product/Bio-Techne corporation
    Average 94 stars, based on 29 article reviews
    histone h2ax [p ser139] antibody (3f2) - by Bioz Stars, 2026-04
    94/100 stars

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    Novus Biologicals γh2ax mouse antibody
    Doxorubicin treatment induces senescence in human IMR‐90 fibroblasts. (A) Representative images of SA‐βGal‐stained S‐dox and NS with or without the addition of Urolithin A (UA). (B) The proportion of cells staining positive for SA‐βGal in NS and S‐dox cells with and without the UA treatment. Four fields were quantified per well ( n = 3). (C, D) Relative p16 (C) and p21 (D) expression in NS, S‐dox, and RS cells with and without UA treatment, n = 4. (E) IF staining for γ‐H2AX and HMGB1 in NS, S‐dox, and RS cells with or without the addition of UA, 10 days post‐induction. γ‐H2AX—green, HMGB1—red, and DAPI—blue. Scale bar = 30 μm. (F, G) The percentage of cells with two or more γ‐H2AX foci per cell (F) and with nuclear HMGB1 (G). (H–J) Fold change of IL6 (H), IL8 (I), and IL1α (J) gene expression in S‐dox and RS cells relative to NS control. n = 4. All data are presented as mean ± SEM. One‐way ANOVA. ** p < 0.002, *** p < 0.0002 **** p < 0.0001.
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    Novus Biologicals mouse anti γh2ax
    Doxorubicin treatment induces senescence in human IMR‐90 fibroblasts. (A) Representative images of SA‐βGal‐stained S‐dox and NS with or without the addition of Urolithin A (UA). (B) The proportion of cells staining positive for SA‐βGal in NS and S‐dox cells with and without the UA treatment. Four fields were quantified per well ( n = 3). (C, D) Relative p16 (C) and p21 (D) expression in NS, S‐dox, and RS cells with and without UA treatment, n = 4. (E) IF staining for γ‐H2AX and HMGB1 in NS, S‐dox, and RS cells with or without the addition of UA, 10 days post‐induction. γ‐H2AX—green, HMGB1—red, and DAPI—blue. Scale bar = 30 μm. (F, G) The percentage of cells with two or more γ‐H2AX foci per cell (F) and with nuclear HMGB1 (G). (H–J) Fold change of IL6 (H), IL8 (I), and IL1α (J) gene expression in S‐dox and RS cells relative to NS control. n = 4. All data are presented as mean ± SEM. One‐way ANOVA. ** p < 0.002, *** p < 0.0002 **** p < 0.0001.
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    Image Search Results


    Doxorubicin treatment induces senescence in human IMR‐90 fibroblasts. (A) Representative images of SA‐βGal‐stained S‐dox and NS with or without the addition of Urolithin A (UA). (B) The proportion of cells staining positive for SA‐βGal in NS and S‐dox cells with and without the UA treatment. Four fields were quantified per well ( n = 3). (C, D) Relative p16 (C) and p21 (D) expression in NS, S‐dox, and RS cells with and without UA treatment, n = 4. (E) IF staining for γ‐H2AX and HMGB1 in NS, S‐dox, and RS cells with or without the addition of UA, 10 days post‐induction. γ‐H2AX—green, HMGB1—red, and DAPI—blue. Scale bar = 30 μm. (F, G) The percentage of cells with two or more γ‐H2AX foci per cell (F) and with nuclear HMGB1 (G). (H–J) Fold change of IL6 (H), IL8 (I), and IL1α (J) gene expression in S‐dox and RS cells relative to NS control. n = 4. All data are presented as mean ± SEM. One‐way ANOVA. ** p < 0.002, *** p < 0.0002 **** p < 0.0001.

    Journal: Aging Cell

    Article Title: Mitigating Pro‐Inflammatory SASP and DAMP With Urolithin A: A Novel Senomorphic Strategy

    doi: 10.1111/acel.70237

    Figure Lengend Snippet: Doxorubicin treatment induces senescence in human IMR‐90 fibroblasts. (A) Representative images of SA‐βGal‐stained S‐dox and NS with or without the addition of Urolithin A (UA). (B) The proportion of cells staining positive for SA‐βGal in NS and S‐dox cells with and without the UA treatment. Four fields were quantified per well ( n = 3). (C, D) Relative p16 (C) and p21 (D) expression in NS, S‐dox, and RS cells with and without UA treatment, n = 4. (E) IF staining for γ‐H2AX and HMGB1 in NS, S‐dox, and RS cells with or without the addition of UA, 10 days post‐induction. γ‐H2AX—green, HMGB1—red, and DAPI—blue. Scale bar = 30 μm. (F, G) The percentage of cells with two or more γ‐H2AX foci per cell (F) and with nuclear HMGB1 (G). (H–J) Fold change of IL6 (H), IL8 (I), and IL1α (J) gene expression in S‐dox and RS cells relative to NS control. n = 4. All data are presented as mean ± SEM. One‐way ANOVA. ** p < 0.002, *** p < 0.0002 **** p < 0.0001.

    Article Snippet: Primary antibodies used included γH2AX mouse antibody (Novus Biologicals, NB100‐74435), pSTING rabbit antibody (Cell Signaling Technology, 19781S) and anti‐HMGB1 rabbit antibody (Abcam, ab18256).

    Techniques: Staining, Expressing, Gene Expression, Control