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stamp2/steap4 antibody (Bio-Techne corporation)

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Bio-Techne corporation stamp2/steap4 antibody
Stamp2/Steap4 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stamp2/steap4 antibody/product/Bio-Techne corporation
Average 93 stars, based on 6 article reviews

stamp2/steap4 antibody - by Bioz Stars, 2026-04

93/100 stars

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Characterization of STEAP4 as a mitochondrial protein increased in colitis. (A) Schematic diagram of the colonic mitochondrial proteomics analysis in mouse model of colitis. Mice were treated with 3% DSS for 3 d, and then colonic mitochondria were extracted and labeled with a tandem mass tag (TMT) labeling kit for mass spectrometry identification. (B) Histogram for 97 significantly changed novel mitochondrial proteins. Lcn2, lipocalin2; Ltf, lactoferrin; STEAP4, six-transmembrane epithelial antigen of prostate 4; Suclg2, succinate-CoA ligase, GDP-forming, β-subunit. (C) Western blot analysis of STEAP4, mitochondrial marker VDAC1, and cytosolic protein GAPDH after 3% DSS treatment. Ponceau staining was used to examine protein loading levels. Numbers above the blots indicate the mean value normalized with VDAC1. (D) qPCR analysis and (E) representative images of immunofluorescent staining for STEAP4 and epithelial cell marker E-cadherin (E-Cad) in colon tissues from DSS-treated mice (n = 3), UC (n = 8), CD (n = 7), and normal control (NC, n = 8) patients. (Magnification: E, 40×.) *P < 0.05 and **P < 0.01. One-way ANOVA followed by Dunnett’s multiple comparisons test.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Quantitative proteomics identifies STEAP4 as a critical regulator of mitochondrial dysfunction linking inflammation and colon cancer

doi: 10.1073/pnas.1712946114

Figure Lengend Snippet: Characterization of STEAP4 as a mitochondrial protein increased in colitis. (A) Schematic diagram of the colonic mitochondrial proteomics analysis in mouse model of colitis. Mice were treated with 3% DSS for 3 d, and then colonic mitochondria were extracted and labeled with a tandem mass tag (TMT) labeling kit for mass spectrometry identification. (B) Histogram for 97 significantly changed novel mitochondrial proteins. Lcn2, lipocalin2; Ltf, lactoferrin; STEAP4, six-transmembrane epithelial antigen of prostate 4; Suclg2, succinate-CoA ligase, GDP-forming, β-subunit. (C) Western blot analysis of STEAP4, mitochondrial marker VDAC1, and cytosolic protein GAPDH after 3% DSS treatment. Ponceau staining was used to examine protein loading levels. Numbers above the blots indicate the mean value normalized with VDAC1. (D) qPCR analysis and (E) representative images of immunofluorescent staining for STEAP4 and epithelial cell marker E-cadherin (E-Cad) in colon tissues from DSS-treated mice (n = 3), UC (n = 8), CD (n = 7), and normal control (NC, n = 8) patients. (Magnification: E, 40×.) *P < 0.05 and **P < 0.01. One-way ANOVA followed by Dunnett’s multiple comparisons test.

Article Snippet: Antibodies against STEAP4 (NB100-68162) were from Novus; Ltf (sc-53498), Lcn2 (sc-515876), GFP (SC-9996), NQO1 (sc-32793), CHOP (sc-7351), BiP (sc-376768), and GAPDH (SC-25778) were from Santa Cruz Biotechnology; VDAC1 (4866), Nrf2 (12721), p-ERK (4370P), t-ERK(9102), p-AKT(4060), t-AKT(9272), p-STAT3 (9145S), and t-STAT3(9139P) were from Cell Signaling Technology; and nitrotyrosine (05-233) and 4-HNE (AB5605) were from Millipore.

Techniques: Labeling, Mass Spectrometry, Western Blot, Marker, Staining, Control

Steap4 is a direct target gene of HIF-2α. (A) qPCR analysis in HIOs under normoxic or hypoxic conditions. (B) Immunofluorescent costaining for STEAP4 and pimonidazole in the colon tissue from DSS-treated or control mice. (Magnification: Upper Left, 20×; Upper Middle, 20×; Upper Right, 40×; Lower Left, 40×; Lower Middle, 40×; Lower Right, 40×.) (C) qPCR analysis of Steap4 expression in intestinal tissues from VhlF/F (n = 5), VhlΔIE (n = 5), VhlF/F/Hif-2αF/F (n = 5), and VhlΔIE/Hif-2αΔIE (n = 5) mice. (D) Immunofluorescent staining for STEAP4 in the colon tissue from VhlF/F or VhlΔIE mice (n = 3). (Magnification: Upper Left, 20×; Upper Right, 20×; Lower Left, 40×; Lower Right, 40×.) (E) qPCR analysis in the colon tissues from wild-type (n = 3), Hif-1αLSL/LSL (n = 4), and Hif-2αLSL/LSL (n = 4) mice. (F) qPCR analysis and (G) immunofluorescent staining in the colon tissues from Hif-2αF/F (n = 3–5) or Hif-2αΔIE mice (n = 3–6) treated with or without 3% DSS for 3 d. (Magnification: Left, 20×; Right, 20×.) (H) Luciferase promoter activity assay. HCT116 cells were transiently transfected with the Steap4 promoter luciferase construct, and cotransfected with empty vector (EV), HIF-1α, or HIF-2α expression plasmids. (I) Schematic diagram of mouse Steap4 gene promoter depicting two potential putative HREs. Luciferase-reporter constructs under the control of the proximal 5′-flanking region of the mouse Steap4 gene (−2.7, −1.0, or −0.65 kb). (J and K) In vivo ChIP assays were performed on tissue extracts from VhlF/F and VhlKO mice using primers amplifying the proximal 5′-flanking region of the mouse Dmt1 or Steap4 gene. *P < 0.05, **P < 0.01, and ***P < 0.001. NS, not significant. One-way ANOVA followed by Dunnett’s multiple comparisons test or Student’s t test.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Quantitative proteomics identifies STEAP4 as a critical regulator of mitochondrial dysfunction linking inflammation and colon cancer

doi: 10.1073/pnas.1712946114

Figure Lengend Snippet: Steap4 is a direct target gene of HIF-2α. (A) qPCR analysis in HIOs under normoxic or hypoxic conditions. (B) Immunofluorescent costaining for STEAP4 and pimonidazole in the colon tissue from DSS-treated or control mice. (Magnification: Upper Left, 20×; Upper Middle, 20×; Upper Right, 40×; Lower Left, 40×; Lower Middle, 40×; Lower Right, 40×.) (C) qPCR analysis of Steap4 expression in intestinal tissues from VhlF/F (n = 5), VhlΔIE (n = 5), VhlF/F/Hif-2αF/F (n = 5), and VhlΔIE/Hif-2αΔIE (n = 5) mice. (D) Immunofluorescent staining for STEAP4 in the colon tissue from VhlF/F or VhlΔIE mice (n = 3). (Magnification: Upper Left, 20×; Upper Right, 20×; Lower Left, 40×; Lower Right, 40×.) (E) qPCR analysis in the colon tissues from wild-type (n = 3), Hif-1αLSL/LSL (n = 4), and Hif-2αLSL/LSL (n = 4) mice. (F) qPCR analysis and (G) immunofluorescent staining in the colon tissues from Hif-2αF/F (n = 3–5) or Hif-2αΔIE mice (n = 3–6) treated with or without 3% DSS for 3 d. (Magnification: Left, 20×; Right, 20×.) (H) Luciferase promoter activity assay. HCT116 cells were transiently transfected with the Steap4 promoter luciferase construct, and cotransfected with empty vector (EV), HIF-1α, or HIF-2α expression plasmids. (I) Schematic diagram of mouse Steap4 gene promoter depicting two potential putative HREs. Luciferase-reporter constructs under the control of the proximal 5′-flanking region of the mouse Steap4 gene (−2.7, −1.0, or −0.65 kb). (J and K) In vivo ChIP assays were performed on tissue extracts from VhlF/F and VhlKO mice using primers amplifying the proximal 5′-flanking region of the mouse Dmt1 or Steap4 gene. *P < 0.05, **P < 0.01, and ***P < 0.001. NS, not significant. One-way ANOVA followed by Dunnett’s multiple comparisons test or Student’s t test.

Techniques: Control, Expressing, Staining, Luciferase, Activity Assay, Transfection, Construct, Plasmid Preparation, In Vivo

$Overexpression of STEAP4 in the intestine enhances susceptibility to experimental colitis. (A) Serum FD4, (B) H2O2 levels in colonic whole-cell extract (WCE) and mitochondrial (Mito) fraction, and (C) Western blot analysis of colonic tissues in 2-mo-old STEAP transgenic (STEAP4OE) (n = 4) and littermate control (STEAP4+/+) mice (n = 4–6). (D) Body weight, (E) colon lengths, (F) H&E and immunofluorescent staining showing complete loss of epithelium in STEAP4OE but not STEAP4+/+ mice, and pathological score of colon tissues following 7 d of 3% DSS treatment in STEAP4OE (n = 3–12) and STEAP4+/+ mice (n = 3–6). (Magnification: 20×.) (G) H2O2 levels in cytosolic (cyto) and mitochondrial (Mito) fraction and (H) Western blot analysis of intestinal tissues from STEAP4OE (n = 4–6) and STEAP4+/+ (n = 4–6) mice following 7 d of 3% DSS treatment. Numbers above the blots indicate the mean value and SD normalized with GAPDH. *P < 0.05, **P < 0.01, and ***P < 0.001. NS, not significant. Student’s t test.$

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Quantitative proteomics identifies STEAP4 as a critical regulator of mitochondrial dysfunction linking inflammation and colon cancer

doi: 10.1073/pnas.1712946114

Figure Lengend Snippet: Overexpression of STEAP4 in the intestine enhances susceptibility to experimental colitis. (A) Serum FD4, (B) H2O2 levels in colonic whole-cell extract (WCE) and mitochondrial (Mito) fraction, and (C) Western blot analysis of colonic tissues in 2-mo-old STEAP transgenic (STEAP4OE) (n = 4) and littermate control (STEAP4+/+) mice (n = 4–6). (D) Body weight, (E) colon lengths, (F) H&E and immunofluorescent staining showing complete loss of epithelium in STEAP4OE but not STEAP4+/+ mice, and pathological score of colon tissues following 7 d of 3% DSS treatment in STEAP4OE (n = 3–12) and STEAP4+/+ mice (n = 3–6). (Magnification: 20×.) (G) H2O2 levels in cytosolic (cyto) and mitochondrial (Mito) fraction and (H) Western blot analysis of intestinal tissues from STEAP4OE (n = 4–6) and STEAP4+/+ (n = 4–6) mice following 7 d of 3% DSS treatment. Numbers above the blots indicate the mean value and SD normalized with GAPDH. *P < 0.05, **P < 0.01, and ***P < 0.001. NS, not significant. Student’s t test.

Techniques: Over Expression, Western Blot, Transgenic Assay, Control, Staining

$Overexpression of STEAP4 in the intestine leads to mitochondrial iron accumulation. Mineral elements analysis of (A) serum and (B) intestinal tissues, and (C) iron content in mitochondrial (Mito) or cytosolic (Cyto) fraction of intestinal tissues from STEAP4 transgenic (STEAP4OE) (n = 3) and littermate control (STEAP4+/+) (n = 3) mice. *P < 0.05 and **P < 0.01. NS, not significant; two-way ANOVA test.$

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Quantitative proteomics identifies STEAP4 as a critical regulator of mitochondrial dysfunction linking inflammation and colon cancer

doi: 10.1073/pnas.1712946114

Figure Lengend Snippet: Overexpression of STEAP4 in the intestine leads to mitochondrial iron accumulation. Mineral elements analysis of (A) serum and (B) intestinal tissues, and (C) iron content in mitochondrial (Mito) or cytosolic (Cyto) fraction of intestinal tissues from STEAP4 transgenic (STEAP4OE) (n = 3) and littermate control (STEAP4+/+) (n = 3) mice. *P < 0.05 and **P < 0.01. NS, not significant; two-way ANOVA test.

Techniques: Over Expression, Transgenic Assay, Control

Mitochondrial iron restriction reduces the severity of colitis. (A) Body weight change, (B) colon lengths, and (C) H&E staining showing complete loss of epithelium after DSS but not DSS+DFP in STEAP4 transgenic (STEAP4OE) mice, and (D) pathological score of colon tissues for STEAP4OE and littermate control (STEAP4+/+) mice following 7 d of 3% DSS with or without 1 mg/mL DFP treatment. (E) Schematic diagram for establishing a novel microbiota humanized IL10−/− model of chronic colitis. Germ-free IL10−/− mice were inoculated with microbiota from HC or CD patients and 3 wk later H&E staining indicates extensive colitis in CD microbiota treated mice. (F) Gene expression in colons from IL10−/− mice inoculated with microbiota from HC or CD patients. (G) Pathological score and (H) H&E staining of colon tissues from IL10−/− mice at 3 wk after inoculation with microbiota from a CD patient and treated with or without 1 mg/mL DFP treatment for 2 wk. (Magnification: C, 10×; E and G, 20×.) *P < 0.05, **P < 0.01, and ***P < 0.001. Student’s t test or two-way ANOVA test.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Quantitative proteomics identifies STEAP4 as a critical regulator of mitochondrial dysfunction linking inflammation and colon cancer

doi: 10.1073/pnas.1712946114

Figure Lengend Snippet: Mitochondrial iron restriction reduces the severity of colitis. (A) Body weight change, (B) colon lengths, and (C) H&E staining showing complete loss of epithelium after DSS but not DSS+DFP in STEAP4 transgenic (STEAP4OE) mice, and (D) pathological score of colon tissues for STEAP4OE and littermate control (STEAP4+/+) mice following 7 d of 3% DSS with or without 1 mg/mL DFP treatment. (E) Schematic diagram for establishing a novel microbiota humanized IL10−/− model of chronic colitis. Germ-free IL10−/− mice were inoculated with microbiota from HC or CD patients and 3 wk later H&E staining indicates extensive colitis in CD microbiota treated mice. (F) Gene expression in colons from IL10−/− mice inoculated with microbiota from HC or CD patients. (G) Pathological score and (H) H&E staining of colon tissues from IL10−/− mice at 3 wk after inoculation with microbiota from a CD patient and treated with or without 1 mg/mL DFP treatment for 2 wk. (Magnification: C, 10×; E and G, 20×.) *P < 0.05, **P < 0.01, and ***P < 0.001. Student’s t test or two-way ANOVA test.

Techniques: Staining, Transgenic Assay, Control, Gene Expression

STEAP4 is critical for colon tumorigenesis. (A) qPCR analysis and (B) representative immunofluorescent staining images of STEAP4 in colon tissues from 10 paired colorectal tumors and their adjacent normal colon tissues. (C) Kaplan–Meier survival curves of STEAP4 gene in colorectal tumors patients. (D) Steap4 expression in CRCs and adjacent normal tissues from CDX2 Hif-2α F/F/ApcF/+ mice and their littermate controls is shown. Representative (E) endoscopy and gross images of colon tumors, and (F) tumor number, tumor size, tumor burden induced by AOM-DSS protocol in the STEAP4 transgenic (STEAP4OE) and littermate control (STEAP4+/+) mice. (Magnification: B, 40×; E, Upper, 5×, Lower, 8×.) *P < 0.05 and **P < 0.01. Student’s t test.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Quantitative proteomics identifies STEAP4 as a critical regulator of mitochondrial dysfunction linking inflammation and colon cancer

doi: 10.1073/pnas.1712946114

Figure Lengend Snippet: STEAP4 is critical for colon tumorigenesis. (A) qPCR analysis and (B) representative immunofluorescent staining images of STEAP4 in colon tissues from 10 paired colorectal tumors and their adjacent normal colon tissues. (C) Kaplan–Meier survival curves of STEAP4 gene in colorectal tumors patients. (D) Steap4 expression in CRCs and adjacent normal tissues from CDX2 Hif-2α F/F/ApcF/+ mice and their littermate controls is shown. Representative (E) endoscopy and gross images of colon tumors, and (F) tumor number, tumor size, tumor burden induced by AOM-DSS protocol in the STEAP4 transgenic (STEAP4OE) and littermate control (STEAP4+/+) mice. (Magnification: B, 40×; E, Upper, 5×, Lower, 8×.) *P < 0.05 and **P < 0.01. Student’s t test.

Techniques: Staining, Expressing, Transgenic Assay, Control

Mitochondrial iron restriction reduces colon tumorigenesis in STEAP4 transgenic mice. (A) Colon tumor numbers and (B) tumor number grouped by size, (C) tumor burden and (D) quantification of Ki67 staining of colon tumors from STEAP4 transgenic (STEAP4OE) and littermate control (STEAP4+/+) mice treated with AOM-DSS protocol in the presence or absence of 1 mg/mL DFP, 0.064% Tempol in the drinking water. *P < 0.05, **P < 0.01, and ***P < 0.001; two-way ANOVA test.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Quantitative proteomics identifies STEAP4 as a critical regulator of mitochondrial dysfunction linking inflammation and colon cancer

doi: 10.1073/pnas.1712946114

Figure Lengend Snippet: Mitochondrial iron restriction reduces colon tumorigenesis in STEAP4 transgenic mice. (A) Colon tumor numbers and (B) tumor number grouped by size, (C) tumor burden and (D) quantification of Ki67 staining of colon tumors from STEAP4 transgenic (STEAP4OE) and littermate control (STEAP4+/+) mice treated with AOM-DSS protocol in the presence or absence of 1 mg/mL DFP, 0.064% Tempol in the drinking water. *P < 0.05, **P < 0.01, and ***P < 0.001; two-way ANOVA test.

Techniques: Transgenic Assay, Staining, Control

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