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cd81 antibody (1d6) - bsa free  (Bio-Techne corporation)


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    Bio-Techne corporation cd81 antibody (1d6) - bsa free
    Cd81 Antibody (1d6) Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd81 antibody (1d6) - bsa free/product/Bio-Techne corporation
    Average 94 stars, based on 33 article reviews
    cd81 antibody (1d6) - bsa free - by Bioz Stars, 2026-04
    94/100 stars

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    Method validation for small EV (sEV) characterization isolated by size exclusion chromatography. (A) Determination of small EV (sEV)‐enriched fractions by nanoparticle tracking analysis and bicinchoninic acid assay. (B) 1. Western blotting analysis of small EV (sEV)‐enriched markers including CD81, CD63, and CD9 in small EV (sEV)‐enriched sample and positive control sample. 2. Western blotting analysis of contaminant protein including albumin, endosomal markers including Calnexin, GRP94 and loading control including GAPDH in small EV (sEV)‐enriched sample and positive control sample. (C) 1‐6 Transmission electron microscopy images illustrating morphology of small EV (sEV)‐ enriched sample ranging between 67‐138 nm. (A) Singular data points represent individual measurements of small EV (sEV) particle and protein concentration .

    Journal: Molecular Nutrition & Food Research

    Article Title: Effect of Glucoraphanin on the Abundance of Nrf2 Regulated Genes Within Circulating Small Extracellular Vesicles: A Pilot Dietary Intervention

    doi: 10.1002/mnfr.70397

    Figure Lengend Snippet: Method validation for small EV (sEV) characterization isolated by size exclusion chromatography. (A) Determination of small EV (sEV)‐enriched fractions by nanoparticle tracking analysis and bicinchoninic acid assay. (B) 1. Western blotting analysis of small EV (sEV)‐enriched markers including CD81, CD63, and CD9 in small EV (sEV)‐enriched sample and positive control sample. 2. Western blotting analysis of contaminant protein including albumin, endosomal markers including Calnexin, GRP94 and loading control including GAPDH in small EV (sEV)‐enriched sample and positive control sample. (C) 1‐6 Transmission electron microscopy images illustrating morphology of small EV (sEV)‐ enriched sample ranging between 67‐138 nm. (A) Singular data points represent individual measurements of small EV (sEV) particle and protein concentration .

    Article Snippet: Membranes were blocked with 5% non‐fat milk powder in PBS‐T (0.05% vol/vol Tween20 in 1xPBS) for 1 h. Blocked membranes were immunoblotted with CD81 (Novus Biologicals, USA, NB100‐65805, 1:1000, non‐reducing), CD63 (Novus Biologicals, USA, NBP2‐42225, 1:2000, non‐reducing), CD9 (Abcam, ab236630, 1:2000, non‐reducing), GAPDH (Abcam, ab9485, 1:2000, reducing), Calnexin (Abcam, ab22595, 1:2000, reducing), GRP94 (Abcam, ab3674, 1:2000, reducing), and Albumin (Novus Biologicals, USA, NBP1‐32458, 1:1000, reducing) overnight at 4°C.

    Techniques: Biomarker Discovery, Isolation, Size-exclusion Chromatography, Acid Assay, Western Blot, Positive Control, Control, Transmission Assay, Electron Microscopy, Protein Concentration