hif-1 alpha antibody - bsa free  (Bio-Techne corporation)

Journal: Bioactive Materials

Article Title: Bioresponsive and immunotherapeutic nanomaterials to remodel tumor microenvironment for enhanced immune checkpoint blockade

doi: 10.1016/j.bioactmat.2023.10.023

Figure Lengend Snippet: Characterizations of CAT@CaCO 3 NPs and TME remodeling. (A) Particle sizes of CAT@CaCO 3 NPs at pH 7.4, pH 6.5, H 2 O 2 solution (50 mM) or pH 6.5 with H 2 O 2 (50 mM). (B) Zeta-potentials of CAT@CaCO 3 NPs at pH 7.4, pH 6.5, H 2 O 2 solution (50 mM) or pH 6.5 with H 2 O 2 (50 mM). (C) TEM images of CAT@CaCO 3 NPs at pH 7.4 or pH 6.5. (D) pH elevation, (E) H 2 O 2 scavenging, and (F) oxygen generation by different formula of NPs in solution. (G) Intracellular hypoxia levels in 4T1 cells. The fluorescent intensity of Ru (dpp) is negatively correlated with oxygen levels. (H) Semi-quantitative analyses of Western blot analyses of HIF-1 α and β -tubulin in 4T1 cells. (I) In vitro CAT releasing profiles from CAT@CaCO 3 NPs at pH 7.4 or pH 6.5. (J) Catalytic abilities of CAT and CAT@CaCO 3 NPs with or without the presence of protease at pH 7.4 or pH 6.5. *p < 0.05, **p < 0.01, ***p < 0.005. n = 3. Data are presented as mean ± standard error of the mean. Statistical significance was calculated via ANOVA with a Tukey post hoc test for multiple comparisons. a.u., arbitrary unit.

Article Snippet: HIF-1 α and β -tubulin were stained with corresponding primary antibodies (HIF-1 α : NOVUS, Cat no. NB100-654; β -tubulin: Invitrogen, Cat no. PA5-16863) and the secondary antibody (Invitrogen, horseradish peroxidase (HRP)-labeled, Cat no. 65–1620) and shown on the films.

Techniques: Western Blot, In Vitro

Journal: Bioactive Materials

Article Title: Bioresponsive and immunotherapeutic nanomaterials to remodel tumor microenvironment for enhanced immune checkpoint blockade

doi: 10.1016/j.bioactmat.2023.10.023

Figure Lengend Snippet: CAT@CaCO 3 NPs for TME remodeling in vivo. (A) IVIS images of tumors and (B) the ratio of fluorescent intensities at Em 640 nm–580 nm indicating pH in TME after receiving intratumoral injections. The elevated ratio of fluorescent intensities at 640 nm–580 nm indicates the pH elevation. (C) LSCM images and (D) semi-quantitative analyses of ROS levels in tumor tissue after different treatments. (E) LSCM images and (F) semi-quantitative analyses of HIF-1 α expression in frozen sections of tumors after different treatments. (G) Semi-quantitative analyses of the Western blot analysis of HIF-1 α and β -tubulin in tumors after different treatments. Scale bar, 50 μm *p < 0.05, **p < 0.01, ***p < 0.005. n = 3. Data are presented as mean ± standard error of the mean. Statistical significance was calculated via one-way ANOVA with a Tukey post hoc test for multiple comparisons. a.u., arbitrary unit.

Article Snippet: HIF-1 α and β -tubulin were stained with corresponding primary antibodies (HIF-1 α : NOVUS, Cat no. NB100-654; β -tubulin: Invitrogen, Cat no. PA5-16863) and the secondary antibody (Invitrogen, horseradish peroxidase (HRP)-labeled, Cat no. 65–1620) and shown on the films.

Techniques: In Vivo, Expressing, Western Blot