antibody against cd31  (Bio-Techne corporation)

Journal: Sexual Medicine

Article Title: Extracorporeal Shock Waves Therapy Delivered by Aries Improves Erectile Dysfunction in Spontaneously Hypertensive Rats Through Penile Tissue Remodeling and Neovascularization

doi: 10.1016/j.esxm.2019.08.006

Figure Lengend Snippet: Representative images of SHR corpus cavernosum sections immunostained for CD31 and semiquantitative evaluation of CD31. Sections of penis were obtained in sham rats (Panel A) or rats who received the Li-ESWT protocol delivered by Aries (Panel B). Immunopositive cells stained brown (arrows). Magnification: ×40 (left panel), ×400 (right panel). The mean number of dots is presented in Panel C. Li-ESWT = low-intensity extracorporeal shock wave therapy; SHR = spontaneously hypertensive rat.

Article Snippet: Antibody against CD31 (1:500, Mouse Monoclonal CD31/PECAM-1 Antibody; Bio-Techne, Lille Cedex, France) was used as a marker of penile microvascularization.

Techniques: Staining

Journal: Nature Communications

Article Title: A tough nitric oxide-eluting hydrogel coating suppresses neointimal hyperplasia on vascular stent

doi: 10.1038/s41467-021-27368-4

Figure Lengend Snippet: a Schematic illustration for vascular stent deployment in rabbit iliac arteries. b Optical images showing the cross sections of the stented arteries after van Gieson staining. c Quantitative analyses on the cross sections (mean ± SD, n = 12 independent animals). d Confocal laser-scanning microscopy (CLSM) unveiling the endothelialization on the stents (outlined by the dashed lines). The fluorescence intensities of different cell components along the line segments (OA, O’B) in the images were presented (blue: cell nucleus, green: CD31, red: F-actin). Six independent pairs of samples were observed with similar results. e Scanning electron microscopy (SEM) images showing the luminal faces of the stented arteries at 3 months post stent deployment. Three independent pairs of samples were observed with similar results. One-way ANOVA with Tukey post hoc test was performed to determine the difference among various groups and two-tailed Student’s t -test was assumed to determine the difference between two groups. ( n.s. not significant).

Article Snippet: The semi-columnar segments in paraformaldehyde solution were further permeabilized with Triton X-100 solution for 3 h and then blocked with BSA solution for 12 h. Subsequently, they were incubated with mouse anti-rabbit CD31 antibody (IgG, Cat. No.: NBP2-44342, Novus Biologicals, USA, 1/100 dilution in PBS) or mouse anti-porcine CD31 antibody (IgG, Cat. No.: NB100-65336, Novus Biologicals, USA, 1/100 dilution in PBS) for 6 h, and then Alexa Fluor ® 488-conjugated goat anti-mouse IgG secondary antibody (Cat. No.: abs20013, Absin Bioscience, 1/100 dilution in PBS) for another 6 h. After that, they were simultaneously stained with phalloidin-TRITC and DAPI for 6 h. Finally, the samples were photographed using a CLSM (A1 Plus, Nikon, Japan; software: Nikon NIS-Elements AR 5.01.00) after being extensively washed with PBS.

Techniques: Staining, Confocal Laser Scanning Microscopy, Fluorescence, Electron Microscopy, Two Tailed Test

Journal: Nature Communications

Article Title: A tough nitric oxide-eluting hydrogel coating suppresses neointimal hyperplasia on vascular stent

doi: 10.1038/s41467-021-27368-4

Figure Lengend Snippet: a Schematic illustration for vascular stent deployment in porcine coronary arteries. b Digital subtraction angiography prior to the harvest of the stented arteries. The white arrows indicate the sites of implanted stents. The yellow arrow refers to severe restenosis occurring in a polymer-coated stent. c Photographs displaying the luminal faces of the stented coronary arteries at 2 weeks and 3 months post stent deployment. d Optical images showing the cross sections of the stented arteries after van Gieson staining. e Quantitative analyses on the cross-sections (mean ± SD, n = 6 independent animals). f Confocal laser-scanning microscopy (CLSM) unveiling the endothelialization on the stents (outlined by the dashed lines). (blue: cell nucleus, green: CD31, red: F-actin). The endothelial coverages were determined for different types of stents (mean ± SD, n = 6 independent animals). g Scanning electron microscopy (SEM) images showing the luminal faces of the stented arteries at 2 weeks and 3 months post stent deployment. h Correlation between endothelialization and neointimal hyperplasia. The increments in neointimal thickness between 2 weeks and 3 months post stent deployment were plotted versus the mean endothelial coverage during that period of time (mean ± SEM, n = 6 independent animals). One-way ANOVA with Tukey post hoc test was performed to determine the difference among various groups ( #### P < 0.0001 compared with other groups).

Article Snippet: The semi-columnar segments in paraformaldehyde solution were further permeabilized with Triton X-100 solution for 3 h and then blocked with BSA solution for 12 h. Subsequently, they were incubated with mouse anti-rabbit CD31 antibody (IgG, Cat. No.: NBP2-44342, Novus Biologicals, USA, 1/100 dilution in PBS) or mouse anti-porcine CD31 antibody (IgG, Cat. No.: NB100-65336, Novus Biologicals, USA, 1/100 dilution in PBS) for 6 h, and then Alexa Fluor ® 488-conjugated goat anti-mouse IgG secondary antibody (Cat. No.: abs20013, Absin Bioscience, 1/100 dilution in PBS) for another 6 h. After that, they were simultaneously stained with phalloidin-TRITC and DAPI for 6 h. Finally, the samples were photographed using a CLSM (A1 Plus, Nikon, Japan; software: Nikon NIS-Elements AR 5.01.00) after being extensively washed with PBS.

Techniques: Polymer, Staining, Confocal Laser Scanning Microscopy, Electron Microscopy