Journal: Biology of reproduction

Article Title: Identification and characterization of a novel CD2-positive cell population in the seminiferous tubule of Fischer CDF344 rats

doi: 10.1093/biolre/ioaf162

Figure Lengend Snippet: Super-resolution photomicrographs of CD2 + cells (green, applies to all the images in the panel) from whole-mount seminiferous tubule preparations from the corn oil-treated peripubertal rats, imaged at 60×—A) CD2 + single cells uniquely observed with a higher nuclear-to-cytoplasmic ratio (relatively large nuclei with a thin rim of cytoplasm) and serrated border. (B) CD2 + clone of two cells. (C) Clone of three cells. Confocal photomicrographs of the whole-mount seminiferous tubule preparations from the corn oil-treated peripubertal rats. (D) Dotted lines highlighting structures that look like cytoplasmic projections interconnecting the CD2 + clone of two cells with a single cell. (E) Cluster of CD2 + cells showing markedly different nuclei morphology, size, and cytoplasmic partition. (F) Occasionally observed noticeably small CD2 + single cells measuring in size around 6.8–7 μ m. (G) CD2 + cell nuclei tested negative for Ki67 (cell proliferation marker) protein (red). Scale bars: 15 μ m in (A), 5 μ m in (B), and 10 μ m in (C); 15 μ m in (D–G).

Article Snippet: CD2 , Novus Biologicals , NB100-65228DL594.

Techniques: Marker

Journal: Biology of reproduction

Article Title: Identification and characterization of a novel CD2-positive cell population in the seminiferous tubule of Fischer CDF344 rats

doi: 10.1093/biolre/ioaf162

Figure Lengend Snippet: Confocal photomicrographs were taken at 60× magnification, and seminiferous tubules collected from the corn oil–treated rats were utilized for staining and microscopy. Co-staining of CD2 + cells (green, applies to all the images in the panel) and (A) Sertoli cell marker (SOX9, red). (B) Pan germ cell marker (DDX4, red). (C) Undifferentiated and differentiating spermatogonia (PLZF, red). (D) Differentiated spermatogonia (STRA8, red). (E) Seminiferous tubules collected from the corn oil–treated rats were co-localized for CD2 (green) and CD45 (red) protein, z-depth was 2.6 μ m from the surface of the tubule, CD2 + cells do not co-localize with the CD45 protein, the arrow points to a distinct CD45 + cell within the PTMC layer. (F) Seminiferous tubules collected from the MEHP-treated rats were co-localized for CD2 (green) and CD45 (red), CD2 + cells lack co-localization with the CD45 protein, presence of CD45 + cell is highlighted with an arrow mark, and z-depth was 2.8 μ m. The z-depth varied for the images of (A–D), based on the depth of the desired cell being imaged, scale bar—15 μ m applies to all the images in this panel.

Article Snippet: CD2 , Novus Biologicals , NB100-65228DL594.

Techniques: Staining, Microscopy, Marker

Journal: Biology of reproduction

Article Title: Identification and characterization of a novel CD2-positive cell population in the seminiferous tubule of Fischer CDF344 rats

doi: 10.1093/biolre/ioaf162

Figure Lengend Snippet: Confocal photomicrographs taken at 60× magnification, seminiferous tubules collected from the corn oil–treated rats utilized for the staining and microscopy, and colored text on the image refers to the immunolabeled entity. (A–D) CD2 cells + are present within the PTMC layer, and their cell shape is irregular; primarily, the CD2 + cells’ shape is determined by the cellular space between the PTMCs. Arrow marks highlight the spot of the empty cellular space between the PTMCs where CD2 + cells localize. (E–G) Arrow marks highlight the spot of the cellular space between the PTMCs where MHC II + PTM φ localizes. Based on the characteristic nuclear morphology, the asterisk symbol points to the nucleus, which is presumed to be a CD2 + cell. (H) Cell-to-cell interaction between an MHC II + PTM φ and CD2 + cell, blue—nuclei. (H′) Masked and surface rendered image visualizing the type of cellular interaction where the membrane of the CD2 + cell is extended or pinched by the PTM φ , nearly half of the CD2 + cell surface was overlapped with PTM φ surface. (I) Another type of cell-to-cell contact. (I) Surface-rendered image shows a clear cell-to-cell contact between MHC II + PTM φ and CD2 + cell, where the membrane of PTM φ overlaps with the boundary of the CD2 + cell membrane. Scale bar: 15 μ m in (A–G).

Article Snippet: CD2 , Novus Biologicals , NB100-65228DL594.

Techniques: Staining, Microscopy, Immunolabeling, Membrane

Journal: Biology of reproduction

Article Title: Identification and characterization of a novel CD2-positive cell population in the seminiferous tubule of Fischer CDF344 rats

doi: 10.1093/biolre/ioaf162

Figure Lengend Snippet: (A, B) Representative confocal photomicrographs of whole mount seminiferous tubules from rats at 48 h post-exposure, green—PTM φ labeled for MHC II protein, n = 5. (A) Corn oil. (B) MEHP treatment group. (C) Graph presenting the average number of PTM φ per 10 5 area, MEHP-exposed animals showed a significant increase in MHC II + PTM φ s, calculating changes in PTM φ s number with n = 5 rats per treatment group. (D) Representative confocal photomicrograph of whole-mount seminiferous tubules from rats 48 h post-exposure from the corn oil–treated animals (red—CD2 + cells and green—PTM φ labeled for MHC II protein). (E) A representative confocal photomicrograph from the MEHP treatment. (F) Graph presenting the average ± SEM (standard error of the mean) of PTM φ s-CD2 + cells (red) interaction per 10 5 area. The average number of cellular interactions between the PTM φ s and CD2 + cells increased significantly in the MEHP-exposure group when compared to the corn oil–treated group, ( n = 5/group). Scale bar: 15 μ m applies to all the images in this panel.

Article Snippet: CD2 , Novus Biologicals , NB100-65228DL594.

Techniques: Labeling

Journal: Biology of reproduction

Article Title: Identification and characterization of a novel CD2-positive cell population in the seminiferous tubule of Fischer CDF344 rats

doi: 10.1093/biolre/ioaf162

Figure Lengend Snippet: Representative photomicrographs showing the different CD2 + (green) cell clones, images were taken at 60× magnification, scale bar—15 μ m. (A) Corn oil group, 48 h post-exposure. (B) MEHP treatment group, 48 h post-exposure. (C) Corn oil group, 2 weeks post-exposure. (D) MEHP treatment group, 2 weeks post-exposure. (E) Graph presenting the average ± SEM of CD2 + cell clones per 10 5 area, MEHP exposure did not affect the average number of the CD2 + cells when compared to the respective corn oil–treated data within the categories: single cell ( P = 0.30), clone of two ( P = 0.54), and clone of three ( P = 0.59) at 48 h post-exposure. For the categories: single cell ( P = 0.84), a clone of two ( P = 0.07), and a clone of three ( P = 0.29) at 2 weeks post-exposure. The data were analyzed to be statistically not significant (at P < 0.05), ( n = 5/group).

Article Snippet: CD2 , Novus Biologicals , NB100-65228DL594.

Techniques: Clone Assay