Journal: bioRxiv
Article Title: Adenine Base Editing Potently Suppresses Hepatitis B Surface Antigen Expression and Inhibits Hepatitis D Virus Release
doi: 10.64898/2026.02.06.704371
Figure Lengend Snippet: (A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot using anti-HBs monoclonal (H166, Abbot) or polyclonal antibody (NB100-62652, NovusBio). Ponceau staining of total protein was used to control protein loading.
Article Snippet: Immunodetection was performed using primary antibodies including monoclonal anti-HBs (Abbott H166 mouse), polyclonal anti-HBs (Novus biologicals NB100-62652 rabbit), anti-Ku80 (ab119935, mouse, Abcam), anti-HDV (homemade, mouse), followed by incubation with horseradish peroxidase (HRP) or fluorophore-conjugated secondary antibodies.
Techniques: Infection, Enzyme-linked Immunosorbent Assay, Control, Western Blot, Staining