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p300 mouse monoclonal antibody (Novus Biologicals)

Novus Biologicals p300 mouse monoclonal antibody
P300 Mouse Monoclonal Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p300 mouse monoclonal antibody/product/Novus Biologicals
Average 85 stars, based on 1 article reviews

p300 mouse monoclonal antibody - by Bioz Stars, 2026-04

85/100 stars

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anti p300 (Novus Biologicals)

Novus Biologicals anti p300

Decrease in acetylated histone H4 and the binding level of <t>p300</t> to the promoter of p27 H. pylori infected cells. The acetylation status of histone H4 at the p27 promoter was monitored with ChIP assays using an anti-acetyl H4 antibody in HS3C (A) and AGS cells that were infected with H. pylori for 6 h (B). The ChIP experiments were conducted using an antibody against p300 in HS3C cells (C) and following acute H. pylori infection in AGS cells (D). The endogenous p27 promoter DNA that co-precipitated with the anti-acetyl H4 and p300 antibody was quantified by PCR and normalised to the corresponding input controls. Antibody negative (Ab−) controls are shown in the right panel of each figure. The summary data are shown in the graphs at the bottom. The data represent the mean ± SD of 3 independent experiments with **P < 0.01 versus the corresponding control.

Anti P300, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p300/product/Novus Biologicals
Average 90 stars, based on 1 article reviews

anti p300 - by Bioz Stars, 2026-04

90/100 stars

Buy from Supplier

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Decrease in acetylated histone H4 and the binding level of p300 to the promoter of p27 H. pylori infected cells. The acetylation status of histone H4 at the p27 promoter was monitored with ChIP assays using an anti-acetyl H4 antibody in HS3C (A) and AGS cells that were infected with H. pylori for 6 h (B). The ChIP experiments were conducted using an antibody against p300 in HS3C cells (C) and following acute H. pylori infection in AGS cells (D). The endogenous p27 promoter DNA that co-precipitated with the anti-acetyl H4 and p300 antibody was quantified by PCR and normalised to the corresponding input controls. Antibody negative (Ab−) controls are shown in the right panel of each figure. The summary data are shown in the graphs at the bottom. The data represent the mean ± SD of 3 independent experiments with **P < 0.01 versus the corresponding control.

Journal: Cancer letters

Article Title: Helicobacter pylori decreases p27 expression through the delta opioid receptor-mediated inhibition of histone acetylation within the p27 promoter

doi: 10.1016/j.canlet.2012.07.032

Figure Lengend Snippet: Decrease in acetylated histone H4 and the binding level of p300 to the promoter of p27 H. pylori infected cells. The acetylation status of histone H4 at the p27 promoter was monitored with ChIP assays using an anti-acetyl H4 antibody in HS3C (A) and AGS cells that were infected with H. pylori for 6 h (B). The ChIP experiments were conducted using an antibody against p300 in HS3C cells (C) and following acute H. pylori infection in AGS cells (D). The endogenous p27 promoter DNA that co-precipitated with the anti-acetyl H4 and p300 antibody was quantified by PCR and normalised to the corresponding input controls. Antibody negative (Ab−) controls are shown in the right panel of each figure. The summary data are shown in the graphs at the bottom. The data represent the mean ± SD of 3 independent experiments with **P < 0.01 versus the corresponding control.

Article Snippet: Reagents The antibodies used in this study were the following: mouse monoclonal anti-p27 kip1 (clone 57; BD Biosciences), anti-β-actin (clone AC-74; Sigma-Aldrich), anti-p300 (clone RW105; Novus Biologicals), rabbit polyclonal anti-DOR (ab66318; Abcam), anti-phospho-DOR (Ser363) (ab62152; Abcam), and anti-Ac-H4 (06–866; Millipore).

Techniques: Binding Assay, Infection, Control

Changes in the binding of p300 at the p27 promoter following treatment with DPDPE. The binding levels of p300 to the p27 promoter were monitored with ChIP assays using an anti-p300 antibody and following treatment with 0.3 μM DPDPE (DP) for 1 h in HS3C cells (A) and AGS cells following acute H. pylori infection for 6 h (B). The endogenous p27 promoter that co-precipitated with the anti-p300 antibody was quantified by PCR, and the data were normalised to the corresponding input controls. The panels on the right are antibody negative (Ab−) controls. The data represent the mean ± SD of 3 independent experiments with **P < 0.01 versus the corresponding control.

Journal: Cancer letters

Article Title: Helicobacter pylori decreases p27 expression through the delta opioid receptor-mediated inhibition of histone acetylation within the p27 promoter

doi: 10.1016/j.canlet.2012.07.032

Figure Lengend Snippet: Changes in the binding of p300 at the p27 promoter following treatment with DPDPE. The binding levels of p300 to the p27 promoter were monitored with ChIP assays using an anti-p300 antibody and following treatment with 0.3 μM DPDPE (DP) for 1 h in HS3C cells (A) and AGS cells following acute H. pylori infection for 6 h (B). The endogenous p27 promoter that co-precipitated with the anti-p300 antibody was quantified by PCR, and the data were normalised to the corresponding input controls. The panels on the right are antibody negative (Ab−) controls. The data represent the mean ± SD of 3 independent experiments with **P < 0.01 versus the corresponding control.

Techniques: Binding Assay, Infection, Control

Effects of H. pylori infection on phospho-DOR and p27. Western blot analyses of phospho-DOR, DOR, p300, and Ac-H4 protein levels in whole-cell lysates of HS3C cells and AGS cells that were infected with H. pylori for 6 h (A). Actin was used as a loading control. Immunohistochemical staining of phospho-DOR and p27 was conducted on the gastric mucosa of 36 patients before and after the eradication of H. pylori. The mean expression scores of phospho-DOR and p27 in 36 patients before and after H. pylori eradication (B). Here, *P < 0.05 and **P < 0.01 versus the corresponding control. Representative immunohistochemical staining of phospho-DOR and p27 expression in gastric epithelial cells (C). Magnification X400. The correlation between phospho-DOR and p27 expression was analysed (D).

Journal: Cancer letters

Article Title: Helicobacter pylori decreases p27 expression through the delta opioid receptor-mediated inhibition of histone acetylation within the p27 promoter

doi: 10.1016/j.canlet.2012.07.032

Figure Lengend Snippet: Effects of H. pylori infection on phospho-DOR and p27. Western blot analyses of phospho-DOR, DOR, p300, and Ac-H4 protein levels in whole-cell lysates of HS3C cells and AGS cells that were infected with H. pylori for 6 h (A). Actin was used as a loading control. Immunohistochemical staining of phospho-DOR and p27 was conducted on the gastric mucosa of 36 patients before and after the eradication of H. pylori. The mean expression scores of phospho-DOR and p27 in 36 patients before and after H. pylori eradication (B). Here, *P < 0.05 and **P < 0.01 versus the corresponding control. Representative immunohistochemical staining of phospho-DOR and p27 expression in gastric epithelial cells (C). Magnification X400. The correlation between phospho-DOR and p27 expression was analysed (D).

Techniques: Infection, Western Blot, Control, Immunohistochemical staining, Staining, Expressing

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