nb100-60554  (Bio-Techne corporation)

Journal: iScience

Article Title: Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice

doi: 10.1016/j.isci.2022.105769

Figure Lengend Snippet: CCDC92 is responsive to high-fat diet feeding in vivo (A) Representative immunohistochemical (IHC) images of CCDC92 in normal human white adipose tissue (WAT). Scale bar = 20 μm. (B) Microarray gene expression analysis of epididymal white adipose tissue (eWAT) in mice on a normal chow diet (ND) or high-fat diet (HFD) from the dataset ( GSE32095 ). (C) Microarray analysis of inguinal adipose tissue (iWAT) from mice on an ND or HFD ( GSE4692 ). Heatmaps in B and C show the regulated genes related to lipid metabolism. (D-F) Male C57BL/6N mice were fed an ND or HFD for 14 weeks (D) mRNA expression of Ccdc92 in eWAT and subcutaneous white adipose tissue (sWAT), n = 4/group. (E) Representative immunoblots and quantification of CCDC92 in eWAT, n = 4/group. (F) Representative images show CCDC92 colocalization with Perilipin (adipose tissue marker), CD44 (mesenchymal stem cell marker), and CD68 (macrophage marker) in eWAT from C57BL/6N mice on HFD. Colocalization was quantified by Pearson’s correlation coefficient (PCC). Scale bar = 50 μm. Data are presented as mean ± SEM, ∗p < 0.05; ∗∗p < 0.01. Analysis in (D-F) used unpaired two-tailed t -test.

Article Snippet: For immunostaining, mouse tissue sections embedded in paraffin were incubated with primary antibodies, including CD44 (BioLegend #103002, 1:200), CD68 (Santa Cruz #sc-20060, 1:200), Perilipin (Novus #NB100-60554, 1:200), or UCP1 (ABclonal, A5857, 1:200) overnight at 4°C, followed by incubation with a fluorescent-dye (Alexa Fluor 594- or Alexa Fluor 488; 1:1,000)-conjugated or enzyme (Alkaline phosphatase, 1:1,000)-conjugated secondary antibody.

Techniques: In Vivo, Immunohistochemical staining, Microarray, Gene Expression, Expressing, Western Blot, Marker, Two Tailed Test

Journal: iScience

Article Title: Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice

doi: 10.1016/j.isci.2022.105769

Figure Lengend Snippet:

Article Snippet: For immunostaining, mouse tissue sections embedded in paraffin were incubated with primary antibodies, including CD44 (BioLegend #103002, 1:200), CD68 (Santa Cruz #sc-20060, 1:200), Perilipin (Novus #NB100-60554, 1:200), or UCP1 (ABclonal, A5857, 1:200) overnight at 4°C, followed by incubation with a fluorescent-dye (Alexa Fluor 594- or Alexa Fluor 488; 1:1,000)-conjugated or enzyme (Alkaline phosphatase, 1:1,000)-conjugated secondary antibody.

Techniques: Derivative Assay, Virus, Recombinant, Lysis, Extraction, Immunoprecipitation, Magnetic Beads, Protease Inhibitor, Luciferase, SYBR Green Assay, Membrane, Bradford Assay, Saline, Staining, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Sequencing, RNA Sequencing, Software