Review





Similar Products

nac1 antibody  (Novus Biologicals)
90
Novus Biologicals nac1 antibody
Silencing of Nucleus accumbens-1 <t>(NAC1)</t> expression activates mitochondrial function in hypoxic tumor cells. (A) HeLa cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in normoxia or hypoxia (1% O 2 ) for 24 h. Mitochondrial mass was estimated by nonyl acridine orange (NAO) staining. Fluorescence was measured with flow cytometry. Bars are mean ± SD representing the results for hypoxia-treated samples of three independent experiments. (B) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in normoxia or hypoxia (1% O 2 ) for 24 h. Oxygen consumption was measured in full, glucose-free, or glutamine-free media by using an oxygen electrode, and normalized to cell numbers. Bars are mean ± SD of three independent experiments. (C) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in normoxia or hypoxia (1% O 2 ) for 24 h. Mitochondrial membrane potential (ΔΨm) was assayed by JC-1 staining flow cytometry. Bars are mean ± SD of three independent experiments. ∗∗ p < 0.01.
Nac1 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nac1 antibody/product/Novus Biologicals
Average 90 stars, based on 1 article reviews
nac1 antibody - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Silencing of Nucleus accumbens-1 (NAC1) expression activates mitochondrial function in hypoxic tumor cells. (A) HeLa cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in normoxia or hypoxia (1% O 2 ) for 24 h. Mitochondrial mass was estimated by nonyl acridine orange (NAO) staining. Fluorescence was measured with flow cytometry. Bars are mean ± SD representing the results for hypoxia-treated samples of three independent experiments. (B) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in normoxia or hypoxia (1% O 2 ) for 24 h. Oxygen consumption was measured in full, glucose-free, or glutamine-free media by using an oxygen electrode, and normalized to cell numbers. Bars are mean ± SD of three independent experiments. (C) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in normoxia or hypoxia (1% O 2 ) for 24 h. Mitochondrial membrane potential (ΔΨm) was assayed by JC-1 staining flow cytometry. Bars are mean ± SD of three independent experiments. ∗∗ p < 0.01.

Journal: Frontiers in Pharmacology

Article Title: Silencing of NAC1 Expression Induces Cancer Cells Oxidative Stress in Hypoxia and Potentiates the Therapeutic Activity of Elesclomol

doi: 10.3389/fphar.2017.00804

Figure Lengend Snippet: Silencing of Nucleus accumbens-1 (NAC1) expression activates mitochondrial function in hypoxic tumor cells. (A) HeLa cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in normoxia or hypoxia (1% O 2 ) for 24 h. Mitochondrial mass was estimated by nonyl acridine orange (NAO) staining. Fluorescence was measured with flow cytometry. Bars are mean ± SD representing the results for hypoxia-treated samples of three independent experiments. (B) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in normoxia or hypoxia (1% O 2 ) for 24 h. Oxygen consumption was measured in full, glucose-free, or glutamine-free media by using an oxygen electrode, and normalized to cell numbers. Bars are mean ± SD of three independent experiments. (C) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in normoxia or hypoxia (1% O 2 ) for 24 h. Mitochondrial membrane potential (ΔΨm) was assayed by JC-1 staining flow cytometry. Bars are mean ± SD of three independent experiments. ∗∗ p < 0.01.

Article Snippet: Antibodies to PDK3, PARP, caspase3, and β-actin were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, United States); NAC1 antibody was obtained from Novus Biologicals LLC (Littleton, CO, United States).

Techniques: Expressing, Transfection, Incubation, Staining, Fluorescence, Flow Cytometry, Membrane

Nucleus accumbens-1 controls mitochondrial respiration via pyruvate dehydrogenase kinase 3 (PDK3) in hypoxia. (A) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in hypoxia (1% O 2 ) for 24 h. PDK1–4 isoforms mRNA expression levels were measured by using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), and plotted after normalization. Bars are mean ± SD of three independent experiments. HeLa and SKOV3 cells were subjected to same treatment as above and PDK3 protein expression levels were examined by Western Blot, with β-actin as a loading control. Data shown are the representative of three identical experiments. (B) HeLa cells with silencing of NAC1 expression with or without HIF-1α plasmid were transfected with PDK3 or HRE mutated PDK3 (PDK3-mut) reporter constructs, 12 h later, the cells were then incubated in hypoxia (1% O 2 ) for additional 24 h. Luciferase activity of the PDK3 promoter was measured by using the Dual-Luciferase Reporter Assay system, and plotted after normalization with the activity of Renilla luciferase. Bars are mean ± SD of three independent experiments. (C) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in hypoxia (1% O 2 ) for 24 h. PDH activity was measured by using PDH Assay Kit. Microplate-recorded data are representative of three independent experiments. (D) HeLa and SKOV3 cells with silencing of NAC1 expression were transfected with or without PDK3 plasmid for 12 h, and then exposed to hypoxia for additional 24 h. Oxygen consumption was measured in full, glucose-free, or glutamine-free media by using an oxygen electrode, and normalized to cell numbers. Bars are mean ± SD of three independent experiments. (E) Mice inoculated with HeLa cells expressing NAC1-targeted shRNA were infected with or without PDK3 virus. Glucose uptake ( 18 F-FDG) was analyzed by microPET. Images of white squares point to tumors. Data are shown as mean ± SD of n = 5 mice per group. ∗∗ p < 0.01.

Journal: Frontiers in Pharmacology

Article Title: Silencing of NAC1 Expression Induces Cancer Cells Oxidative Stress in Hypoxia and Potentiates the Therapeutic Activity of Elesclomol

doi: 10.3389/fphar.2017.00804

Figure Lengend Snippet: Nucleus accumbens-1 controls mitochondrial respiration via pyruvate dehydrogenase kinase 3 (PDK3) in hypoxia. (A) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in hypoxia (1% O 2 ) for 24 h. PDK1–4 isoforms mRNA expression levels were measured by using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), and plotted after normalization. Bars are mean ± SD of three independent experiments. HeLa and SKOV3 cells were subjected to same treatment as above and PDK3 protein expression levels were examined by Western Blot, with β-actin as a loading control. Data shown are the representative of three identical experiments. (B) HeLa cells with silencing of NAC1 expression with or without HIF-1α plasmid were transfected with PDK3 or HRE mutated PDK3 (PDK3-mut) reporter constructs, 12 h later, the cells were then incubated in hypoxia (1% O 2 ) for additional 24 h. Luciferase activity of the PDK3 promoter was measured by using the Dual-Luciferase Reporter Assay system, and plotted after normalization with the activity of Renilla luciferase. Bars are mean ± SD of three independent experiments. (C) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated in hypoxia (1% O 2 ) for 24 h. PDH activity was measured by using PDH Assay Kit. Microplate-recorded data are representative of three independent experiments. (D) HeLa and SKOV3 cells with silencing of NAC1 expression were transfected with or without PDK3 plasmid for 12 h, and then exposed to hypoxia for additional 24 h. Oxygen consumption was measured in full, glucose-free, or glutamine-free media by using an oxygen electrode, and normalized to cell numbers. Bars are mean ± SD of three independent experiments. (E) Mice inoculated with HeLa cells expressing NAC1-targeted shRNA were infected with or without PDK3 virus. Glucose uptake ( 18 F-FDG) was analyzed by microPET. Images of white squares point to tumors. Data are shown as mean ± SD of n = 5 mice per group. ∗∗ p < 0.01.

Article Snippet: Antibodies to PDK3, PARP, caspase3, and β-actin were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, United States); NAC1 antibody was obtained from Novus Biologicals LLC (Littleton, CO, United States).

Techniques: Transfection, Incubation, Expressing, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Plasmid Preparation, Construct, Luciferase, Activity Assay, Reporter Assay, shRNA, Infection, Virus

Silencing of NAC1 expression promotes reactive oxygen species (ROS) generation in hypoxic cancer cells. (A) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated for 24 h in the presence or absence of NAC (2 mM) under normoxia or hypoxia (1% O 2 ) conditions, and the level of ROS was measured. Bars are mean ± SD of three independent experiments. (B) HeLa and SKOV3 cells with or without silencing of NAC1 expression were transfected with or without PDK3 plasmid for 12 h, and incubated in normoxia or hypoxia (1% O 2 ) for additional 24 h, and the level of ROS was measured. Bars are mean ± SD of three independent experiments. (C) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were treated with 300 nmol/L elesclomol for 12 h, and ROS production was assessed in the presence or absence of 2 mM NAC. Bars are mean ± SD of three independent experiments. (D) HeLa and SKOV3 cells with or without silencing of NAC1 expression were transfected with PDK3 plasmid for 24 h, and then treated with 300 nmol/L elesclomol for additional 12 h, the level of ROS was measured. Bars are mean ± SD of three independent experiments. ∗∗ p < 0.01.

Journal: Frontiers in Pharmacology

Article Title: Silencing of NAC1 Expression Induces Cancer Cells Oxidative Stress in Hypoxia and Potentiates the Therapeutic Activity of Elesclomol

doi: 10.3389/fphar.2017.00804

Figure Lengend Snippet: Silencing of NAC1 expression promotes reactive oxygen species (ROS) generation in hypoxic cancer cells. (A) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated for 24 h in the presence or absence of NAC (2 mM) under normoxia or hypoxia (1% O 2 ) conditions, and the level of ROS was measured. Bars are mean ± SD of three independent experiments. (B) HeLa and SKOV3 cells with or without silencing of NAC1 expression were transfected with or without PDK3 plasmid for 12 h, and incubated in normoxia or hypoxia (1% O 2 ) for additional 24 h, and the level of ROS was measured. Bars are mean ± SD of three independent experiments. (C) HeLa and SKOV3 cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were treated with 300 nmol/L elesclomol for 12 h, and ROS production was assessed in the presence or absence of 2 mM NAC. Bars are mean ± SD of three independent experiments. (D) HeLa and SKOV3 cells with or without silencing of NAC1 expression were transfected with PDK3 plasmid for 24 h, and then treated with 300 nmol/L elesclomol for additional 12 h, the level of ROS was measured. Bars are mean ± SD of three independent experiments. ∗∗ p < 0.01.

Article Snippet: Antibodies to PDK3, PARP, caspase3, and β-actin were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, United States); NAC1 antibody was obtained from Novus Biologicals LLC (Littleton, CO, United States).

Techniques: Expressing, Transfection, Incubation, Plasmid Preparation

Suppression of ROS production shows decreased sensitivity to hypoxia-induced apoptosis. HeLa cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated for 24 h in the presence or absence of NAC (2 mM) under normoxia or hypoxia (1% O 2 ) conditions; HeLa cells with silencing of NAC1 expression were transfected with or without PDK3 plasmid for 12 h, and incubated under normoxia or hypoxia (1% O 2 ) conditions for additional 24 h. Apoptosis was determined by: (A,B) western blot analysis of cleaved PARP and cleaved caspase-3, with β-actin as a loading control and (C,D) flow cytometric analysis of Annexin V staining. Bars are mean ± SD of three independent experiments. ∗∗ p < 0.01.

Journal: Frontiers in Pharmacology

Article Title: Silencing of NAC1 Expression Induces Cancer Cells Oxidative Stress in Hypoxia and Potentiates the Therapeutic Activity of Elesclomol

doi: 10.3389/fphar.2017.00804

Figure Lengend Snippet: Suppression of ROS production shows decreased sensitivity to hypoxia-induced apoptosis. HeLa cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were incubated for 24 h in the presence or absence of NAC (2 mM) under normoxia or hypoxia (1% O 2 ) conditions; HeLa cells with silencing of NAC1 expression were transfected with or without PDK3 plasmid for 12 h, and incubated under normoxia or hypoxia (1% O 2 ) conditions for additional 24 h. Apoptosis was determined by: (A,B) western blot analysis of cleaved PARP and cleaved caspase-3, with β-actin as a loading control and (C,D) flow cytometric analysis of Annexin V staining. Bars are mean ± SD of three independent experiments. ∗∗ p < 0.01.

Article Snippet: Antibodies to PDK3, PARP, caspase3, and β-actin were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, United States); NAC1 antibody was obtained from Novus Biologicals LLC (Littleton, CO, United States).

Techniques: Transfection, Incubation, Expressing, Plasmid Preparation, Western Blot, Control, Staining

Silencing of NAC1 expression promotes anti-tumor activity of elesclomol in vitro and in vivo. (A) HeLa cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were treated with 300 nmol/L elesclomol for 12 h. Cell viability was measured by MTT assay. Bars are mean ± SD of three independent experiments. (B) Apoptosis was determined by western blot analysis of cleaved PARP, with β-actin as a loading control. Data shown are the representative of three identical experiments. (C) The nude mice inoculated with HeLa cells were randomly divided into four groups: (1) control group; (2) siNAC1 group; (3) elesclomol group; (4) elesclomol/siNAC1 group. The tumor sizes were measured with calipers every other day up to 4 weeks. ∗ P < 0.05, ∗∗ P < 0.01, elesclomol/siNAC1 vs elesclomol. Data are shown as mean ± SD of n = 5 mice per group. (D) Images represent immuno-histochemical staining of Ki67 and TUNEL in tumor specimens from different groups. Bars are mean ± SD ( n = 5). The scale bar represents 25 μm.

Journal: Frontiers in Pharmacology

Article Title: Silencing of NAC1 Expression Induces Cancer Cells Oxidative Stress in Hypoxia and Potentiates the Therapeutic Activity of Elesclomol

doi: 10.3389/fphar.2017.00804

Figure Lengend Snippet: Silencing of NAC1 expression promotes anti-tumor activity of elesclomol in vitro and in vivo. (A) HeLa cells transfected with a non-targeting RNA (Ctrl) or NAC1-targeted siRNA were treated with 300 nmol/L elesclomol for 12 h. Cell viability was measured by MTT assay. Bars are mean ± SD of three independent experiments. (B) Apoptosis was determined by western blot analysis of cleaved PARP, with β-actin as a loading control. Data shown are the representative of three identical experiments. (C) The nude mice inoculated with HeLa cells were randomly divided into four groups: (1) control group; (2) siNAC1 group; (3) elesclomol group; (4) elesclomol/siNAC1 group. The tumor sizes were measured with calipers every other day up to 4 weeks. ∗ P < 0.05, ∗∗ P < 0.01, elesclomol/siNAC1 vs elesclomol. Data are shown as mean ± SD of n = 5 mice per group. (D) Images represent immuno-histochemical staining of Ki67 and TUNEL in tumor specimens from different groups. Bars are mean ± SD ( n = 5). The scale bar represents 25 μm.

Article Snippet: Antibodies to PDK3, PARP, caspase3, and β-actin were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, United States); NAC1 antibody was obtained from Novus Biologicals LLC (Littleton, CO, United States).

Techniques: Expressing, Activity Assay, In Vitro, In Vivo, Transfection, MTT Assay, Western Blot, Control, Staining, TUNEL Assay