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Journal: Cell reports

Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome

doi: 10.1016/j.celrep.2021.109806

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Primary antibodies: puromycin (Kerafast #Equation 0001, 1:1000); eIF4A1 (Abcam #31217, 1:1000); eIF4A2 (SCBT #sc-137148, 1:1000); CD98hc (CST #13180S, 1:500); GEF-H1 (Proteintech #24472-1-AP, 1:1000); SPEN (Novus Biologicals #NB100-58799); CEP170 (ThermoFisher Scientific #41-3200, 1:1000); DHX36 (Proteintech #13159-1-AP, 1:1000); DDX39 (Proteintech #11723-1-AP, 1:1000); JNK (CST #4672S, 1:1000); phospho-JNK (CST #4668S, 1:1000); c-JUN (CST #9165S, 1:1000); phospho-c-JUN (CST #9261S, 1:1000); RHOA (CST #2117S, 1:1000); RHO (ThermoFisher Scientific #16116, 1:1000); p130Cas (CST #13846S, 1:1000); phospho-p130Cas (CST #4011S, 1:1000); AKT (CST #4685S, 1:1000); phospho-AKT (CST #4060S, 1:1000); MCL-1 (CST #94296S, 1:1000); CPSF73 (Proteintech #11609-1-AP, 1:1000); eEF1ε1 (Proteintech #10805-1-AP, 1:1000); DDX17 (Proteintech #19910-1-AP); PABPN1 (Proteintech #66807-1-Ig); Histone H3 (CST #4499S, 1:2000); GAPDH (CST #5174T, 1:2000); CYPB (ThermoFisher Scientific #PA1-027A, 1:10000).

Techniques: Plasmid Preparation, Derivative Assay, Recombinant, Protease Inhibitor, cDNA Synthesis, SYBR Green Assay, Mass Spectrometry, Construct, Software