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rela/nfkb p65 antibody (112a1021) - bsa free  (Bio-Techne corporation)


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    Bio-Techne corporation rela/nfkb p65 antibody (112a1021) - bsa free
    Rela/Nfkb P65 Antibody (112a1021) Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rela/nfkb p65 antibody (112a1021) - bsa free/product/Bio-Techne corporation
    Average 93 stars, based on 24 article reviews
    rela/nfkb p65 antibody (112a1021) - bsa free - by Bioz Stars, 2026-04
    93/100 stars

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    Effects of cilastatin on cecal ligation and puncture (CLP)-induced renal nuclear factor-ĸB (NF-κB) activation and interleukin (IL)-6. ( A ) Representative Western blot of NF-ĸB and Phospho-NF-κB <t>p65</t> in the renal cortex. ( B , C ) Respective quantification corrected by the loading control shows increased NF-κB (* p ≤ 0.0001 vs. all groups) and Phospho-NF-κB p65 (* p ≤ 0.0412 vs. all groups) in CLP rats compared with CLP + cilastatin and control groups. Data represent means ± SEM, n = 4–5 animals per group. ( D ) Localization of NF-ĸB through immunofluorescence staining (green) in kidney sections (magnification 20×, bar 50 µm). An increase in staining can be observed in the CLP group (arrows). ( E ) Serum measurement of NF-ĸB. Data represent means ± SEM, n = 5 animals per group, * p ≤ 0.0067 vs. all groups; # p ≤ 0.022 vs. Control + Cil. ( F ) Serum measurement of IL-6. Data represent means ± SEM, n = 6–10 animals per group, * p ≤ 0.033 vs. all groups. CLP enhances both the expression and activation of NF-ĸB in renal tissue and raises the serum levels of NF-ĸB and IL-6. Cilastatin significantly reduced the increase in both molecules. The data were analyzed using ANOVA. Cil: cilastatin; a.u., arbitrary units.
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    Effects of cilastatin on cecal ligation and puncture (CLP)-induced renal nuclear factor-ĸB (NF-κB) activation and interleukin (IL)-6. ( A ) Representative Western blot of NF-ĸB and Phospho-NF-κB <t>p65</t> in the renal cortex. ( B , C ) Respective quantification corrected by the loading control shows increased NF-κB (* p ≤ 0.0001 vs. all groups) and Phospho-NF-κB p65 (* p ≤ 0.0412 vs. all groups) in CLP rats compared with CLP + cilastatin and control groups. Data represent means ± SEM, n = 4–5 animals per group. ( D ) Localization of NF-ĸB through immunofluorescence staining (green) in kidney sections (magnification 20×, bar 50 µm). An increase in staining can be observed in the CLP group (arrows). ( E ) Serum measurement of NF-ĸB. Data represent means ± SEM, n = 5 animals per group, * p ≤ 0.0067 vs. all groups; # p ≤ 0.022 vs. Control + Cil. ( F ) Serum measurement of IL-6. Data represent means ± SEM, n = 6–10 animals per group, * p ≤ 0.033 vs. all groups. CLP enhances both the expression and activation of NF-ĸB in renal tissue and raises the serum levels of NF-ĸB and IL-6. Cilastatin significantly reduced the increase in both molecules. The data were analyzed using ANOVA. Cil: cilastatin; a.u., arbitrary units.
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    Image Search Results


    Effects of cilastatin on cecal ligation and puncture (CLP)-induced renal nuclear factor-ĸB (NF-κB) activation and interleukin (IL)-6. ( A ) Representative Western blot of NF-ĸB and Phospho-NF-κB p65 in the renal cortex. ( B , C ) Respective quantification corrected by the loading control shows increased NF-κB (* p ≤ 0.0001 vs. all groups) and Phospho-NF-κB p65 (* p ≤ 0.0412 vs. all groups) in CLP rats compared with CLP + cilastatin and control groups. Data represent means ± SEM, n = 4–5 animals per group. ( D ) Localization of NF-ĸB through immunofluorescence staining (green) in kidney sections (magnification 20×, bar 50 µm). An increase in staining can be observed in the CLP group (arrows). ( E ) Serum measurement of NF-ĸB. Data represent means ± SEM, n = 5 animals per group, * p ≤ 0.0067 vs. all groups; # p ≤ 0.022 vs. Control + Cil. ( F ) Serum measurement of IL-6. Data represent means ± SEM, n = 6–10 animals per group, * p ≤ 0.033 vs. all groups. CLP enhances both the expression and activation of NF-ĸB in renal tissue and raises the serum levels of NF-ĸB and IL-6. Cilastatin significantly reduced the increase in both molecules. The data were analyzed using ANOVA. Cil: cilastatin; a.u., arbitrary units.

    Journal: International Journal of Molecular Sciences

    Article Title: Cilastatin Attenuates Acute Kidney Injury and Reduces Mortality in a Rat Model of Sepsis

    doi: 10.3390/ijms26167927

    Figure Lengend Snippet: Effects of cilastatin on cecal ligation and puncture (CLP)-induced renal nuclear factor-ĸB (NF-κB) activation and interleukin (IL)-6. ( A ) Representative Western blot of NF-ĸB and Phospho-NF-κB p65 in the renal cortex. ( B , C ) Respective quantification corrected by the loading control shows increased NF-κB (* p ≤ 0.0001 vs. all groups) and Phospho-NF-κB p65 (* p ≤ 0.0412 vs. all groups) in CLP rats compared with CLP + cilastatin and control groups. Data represent means ± SEM, n = 4–5 animals per group. ( D ) Localization of NF-ĸB through immunofluorescence staining (green) in kidney sections (magnification 20×, bar 50 µm). An increase in staining can be observed in the CLP group (arrows). ( E ) Serum measurement of NF-ĸB. Data represent means ± SEM, n = 5 animals per group, * p ≤ 0.0067 vs. all groups; # p ≤ 0.022 vs. Control + Cil. ( F ) Serum measurement of IL-6. Data represent means ± SEM, n = 6–10 animals per group, * p ≤ 0.033 vs. all groups. CLP enhances both the expression and activation of NF-ĸB in renal tissue and raises the serum levels of NF-ĸB and IL-6. Cilastatin significantly reduced the increase in both molecules. The data were analyzed using ANOVA. Cil: cilastatin; a.u., arbitrary units.

    Article Snippet: Sections were then incubated overnight at 4 °C in a humidified chamber with primary antibodies diluted in PBS-T, 4% bovine serum albumin, and 1% host serum in which the secondary antibody was obtained as follows: mouse anti-RelA/NF-κB p65 monoclonal antibody (Novus Biologicals, Minneapolis, MN, USA [dilution 1:100], ref. NB100-56712); mouse anti-TLR4 (25) monoclonal antibody (Santa Cruz Biotechnology [dilution 1:50], ref. sc-293072); and rabbit anti-MyD88 polyclonal antibody (Novus Biologicals [dilution 1:50], ref. NB100-56698).

    Techniques: Ligation, Activation Assay, Western Blot, Control, Immunofluorescence, Staining, Expressing