Review



caspase 3  (Bio-Techne corporation)


Bioz Verified Symbol Bio-Techne corporation is a verified supplier
Bioz Manufacturer Symbol Bio-Techne corporation manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Bio-Techne corporation caspase 3
    Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved <t>caspase</t> <t>3</t> (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.
    Caspase 3, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase 3/product/Bio-Techne corporation
    Average 94 stars, based on 1 article reviews
    caspase 3 - by Bioz Stars, 2026-04
    94/100 stars

    Images

    1) Product Images from "Tumour-on-chip microfluidic platform for assessment of drug pharmacokinetics and treatment response"

    Article Title: Tumour-on-chip microfluidic platform for assessment of drug pharmacokinetics and treatment response

    Journal: Communications Biology

    doi: 10.1038/s42003-021-02526-y

    Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved caspase 3 (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.
    Figure Legend Snippet: Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved caspase 3 (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.

    Techniques Used:



    Similar Products

    caspase 3  (Bio-Techne corporation)
    94
    Bio-Techne corporation caspase 3
    Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved <t>caspase</t> <t>3</t> (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.
    Caspase 3, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase 3/product/Bio-Techne corporation
    Average 94 stars, based on 1 article reviews
    caspase 3 - by Bioz Stars, 2026-04
    94/100 stars
    		  Buy from Supplier
    alexafluortm 594  (Novus Biologicals)
    93
    Novus Biologicals alexafluortm 594
    Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved <t>caspase</t> <t>3</t> (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.
    Alexafluortm 594, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexafluortm 594/product/Novus Biologicals
    Average 93 stars, based on 1 article reviews
    alexafluortm 594 - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    nb10056708af594  (Novus Biologicals)
    93
    Novus Biologicals nb10056708af594
    Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved <t>caspase</t> <t>3</t> (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.
    Nb10056708af594, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nb10056708af594/product/Novus Biologicals
    Average 93 stars, based on 1 article reviews
    nb10056708af594 - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    novus biologics  (Novus Biologicals)
    93
    Novus Biologicals novus biologics
    Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved <t>caspase</t> <t>3</t> (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.
    Novus Biologics, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/novus biologics/product/Novus Biologicals
    Average 93 stars, based on 1 article reviews
    novus biologics - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    nb100 56708af594  (Bio-Techne corporation)
    93
    Bio-Techne corporation nb100 56708af594
    Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved <t>caspase</t> <t>3</t> (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.
    Nb100 56708af594, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nb100 56708af594/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    nb100 56708af594 - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    nb100 56708af594  (Danaher Inc)
    99
    Danaher Inc nb100 56708af594
    Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved <t>caspase</t> <t>3</t> (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.
    Nb100 56708af594, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nb100 56708af594/product/Danaher Inc
    Average 99 stars, based on 1 article reviews
    nb100 56708af594 - by Bioz Stars, 2026-04
    99/100 stars
    		  Buy from Supplier
    nb100 56708af594  (Abcam)
    99
    Abcam nb100 56708af594
    Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved <t>caspase</t> <t>3</t> (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.
    Nb100 56708af594, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nb100 56708af594/product/Abcam
    Average 99 stars, based on 1 article reviews
    nb100 56708af594 - by Bioz Stars, 2026-04
    99/100 stars
    		  Buy from Supplier
    anti cleaved caspase 3 rabbit polyclonal  (Bio-Techne corporation)
    93
    Bio-Techne corporation anti cleaved caspase 3 rabbit polyclonal
    Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved <t>caspase</t> <t>3</t> (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.
    Anti Cleaved Caspase 3 Rabbit Polyclonal, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cleaved caspase 3 rabbit polyclonal/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    anti cleaved caspase 3 rabbit polyclonal - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved caspase 3 (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.

    Journal: Communications Biology

    Article Title: Tumour-on-chip microfluidic platform for assessment of drug pharmacokinetics and treatment response

    doi: 10.1038/s42003-021-02526-y

    Figure Lengend Snippet: Spheroids were treated in the chip with SN38 and AZD0156 at different schedules using the microfluidic setup. Spheroids were recovered from the chip at day 7 and DNA double strand break damage was assessed via the presence of γH2AX. Scalebar = 100 µm. a Representative images of nuclei (blue/Hoechst 33342) and γH2AX (green), b quantification of γH2AX positive cells. (A-SN38, B-SN38 + AZD0156 3/7 with 24 h gap, C-SN38 + AZD0156 3/7 with 72 h gap, D-SN38 + AZD0156 1/7, E-SN38 + AZD0156 7/7, F-control). Cleaved caspase 3 (CC-3) was used to quantified apoptotic cell death ( c ), and Ki67 to measure the effect on proliferation ( d ). N ≥ 5 per condition from two independent experiments. Box-plots show median (centre line); box limits are 25th to 75th percentile; whiskers represent min and max values. Statistical analysis was carried out using 1-way ANOVA, Tukey’s multiple comparisons, CI = 95%, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.1.

    Article Snippet: Then, triple labelling was performed overnight, in static conditions, using primary conjugated antibodies for γH2Ax (gamma H2AX [p Ser139] Antibody [Alexa Fluor 488], NB100-384AF488, Novus Bio/BioTechne), caspase-3 (CC3, Caspase-3 Antibody (31A1067) [Alexa Fluor 594], NB100-56708AF594, and KI67 (Anti-Ki67 antibody [EPR3610] (Alexa Fluor 647), ab196907, Abcam) at 1:500 dilution each.

    Techniques: