Journal: Microscopy and Microanalysis

Article Title: LPS- and LTA-Induced Expression of TLR4, MyD88, and TNF-α in Lymph Nodes of the Akkaraman and Romanov Lambs

doi: 10.1017/s1431927622012314

Figure Lengend Snippet: Fig. 3. TLR4, MyD88, and TNF-α immunoreactivity in the bronchial lymph nodes of Akkaraman lambs in LTA, LPS, LTA + LPS, control groups and negative control (e,j,o). K, capsule; T, trabeculae; LF, lymph follicle; V, blood vessel; Sg, centrum germinativum; Sc, subcapsuler sinus; Mc, medullary cord; arrows, TLR4 immuno- positive smooth muscle cells; arrow heads, TNF-α immunopositive immune cells. Scale bars, 20 μm (a–d,f–i,l–n) and 50 μm (e,j,k,o).

Article Snippet: The sections were incubated at 4 °C overnight with mouse monoclonal TLR4 (Novus Biologicals, CO, USA, NB100-56566, 1:100 dilution), rabbit polyclonal MyD88 (Novus Biologicals, CO, USA, NB100-56598, 1:100 dilution), and mouse monoclonal TNF-α primary antibodies (Bio-Rad Laboratories MCA905GA, 1:75 dilution).

Techniques: Control, Negative Control

Journal: Microscopy and Microanalysis

Article Title: LPS- and LTA-Induced Expression of TLR4, MyD88, and TNF-α in Lymph Nodes of the Akkaraman and Romanov Lambs

doi: 10.1017/s1431927622012314

Figure Lengend Snippet: Fig. 4. TLR4, MyD88, and TNF-α immunoreactivity in the bronchial lymph nodes of Romanov lambs in LTA, LPS, LTA + LPS, control groups, and negative control (e,j, o). K, capsule; T, trabeculae; LF, lymph follicle; V, blood vessel; Mc, medullary cord; Ms, medullary sinus; arrows, TLR4 immunopositive smooth muscle cells; arrow heads, TNF-α immunopositive immune cells. Scale bars, 20 μm (a–d,g–i,l–n) and 50 μm (e,f,j,k,o).

Article Snippet: The sections were incubated at 4 °C overnight with mouse monoclonal TLR4 (Novus Biologicals, CO, USA, NB100-56566, 1:100 dilution), rabbit polyclonal MyD88 (Novus Biologicals, CO, USA, NB100-56598, 1:100 dilution), and mouse monoclonal TNF-α primary antibodies (Bio-Rad Laboratories MCA905GA, 1:75 dilution).

Techniques: Control, Negative Control

Journal: Microscopy and Microanalysis

Article Title: LPS- and LTA-Induced Expression of TLR4, MyD88, and TNF-α in Lymph Nodes of the Akkaraman and Romanov Lambs

doi: 10.1017/s1431927622012314

Figure Lengend Snippet: Fig. 5. TLR4, MyD88, and TNF-α immunoreactivity in the mandibular lymph nodes of Akkaraman lambs in LTA, LPS, LTA + LPS, control groups, and negative control (e,j,o). K, capsule; T, trabeculae; LF, lymph follicle; V, blood vessel; Sc, subcapsuler sinus; Ms, medullary sinus; Mc, medullary cord; arrows, smooth muscle cells; arrow heads, TNF-α immunopositive immune cells. Scale bars, 20 μm (a–d,f–i,k–n) and 50 μm (e,j,o).

Article Snippet: The sections were incubated at 4 °C overnight with mouse monoclonal TLR4 (Novus Biologicals, CO, USA, NB100-56566, 1:100 dilution), rabbit polyclonal MyD88 (Novus Biologicals, CO, USA, NB100-56598, 1:100 dilution), and mouse monoclonal TNF-α primary antibodies (Bio-Rad Laboratories MCA905GA, 1:75 dilution).

Techniques: Control, Negative Control

Journal: Microscopy and Microanalysis

Article Title: LPS- and LTA-Induced Expression of TLR4, MyD88, and TNF-α in Lymph Nodes of the Akkaraman and Romanov Lambs

doi: 10.1017/s1431927622012314

Figure Lengend Snippet: Fig. 6. TLR4, MyD88, and TNF-α immunoreactivity in the mandibular lymph nodes of Romanov lambs in LTA, LPS, LTA + LPS, control groups, and negative control (e,j,o). K, capsule; T, trabeculae; LF, lymph follicle; V, blood vessel; Sg, centrum germinativum; arrows, TLR4 immunopositive smooth muscle cells. Scale bars, 20 μm (a–d,g–i,l–n) and 50 μm (e,f,j,k,o).

Article Snippet: The sections were incubated at 4 °C overnight with mouse monoclonal TLR4 (Novus Biologicals, CO, USA, NB100-56566, 1:100 dilution), rabbit polyclonal MyD88 (Novus Biologicals, CO, USA, NB100-56598, 1:100 dilution), and mouse monoclonal TNF-α primary antibodies (Bio-Rad Laboratories MCA905GA, 1:75 dilution).

Techniques: Control, Negative Control

Journal: Microscopy and Microanalysis

Article Title: LPS- and LTA-Induced Expression of TLR4, MyD88, and TNF-α in Lymph Nodes of the Akkaraman and Romanov Lambs

doi: 10.1017/s1431927622012314

Figure Lengend Snippet: Fig. 7. Western blot analysis of TLR4, MyD88, and TNF-α proteins in the bronchial (a–c) and mandibular (d–f) lymph nodes of Akkaraman and Romanov lambs. Bronchial and mandibular lymph node tissues were obtained from LTA, LPS, LTA + LPS, and control groups (c). Expressions of TLR4, MyD88, and TNF-α molecules were assessed by Western blot as described in the Method section. The graphs below the representative blots show densitometric quantitation of protein bands. Beta actin was used as an internal loading control protein. Data are the mean ± SEM of three replicates in each group.

Article Snippet: The sections were incubated at 4 °C overnight with mouse monoclonal TLR4 (Novus Biologicals, CO, USA, NB100-56566, 1:100 dilution), rabbit polyclonal MyD88 (Novus Biologicals, CO, USA, NB100-56598, 1:100 dilution), and mouse monoclonal TNF-α primary antibodies (Bio-Rad Laboratories MCA905GA, 1:75 dilution).

Techniques: Western Blot, Control, Quantitation Assay

Journal: Microscopy and Microanalysis

Article Title: LPS- and LTA-Induced Expression of TLR4, MyD88, and TNF-α in Lymph Nodes of the Akkaraman and Romanov Lambs

doi: 10.1017/s1431927622012314

Figure Lengend Snippet: Fig. 8. Gene expression levels of TLR4, MyD88, andTNF-α in mandibular and bronchial lymph nodes of LTA, LPS, LTA + LPS, and control groups of Akkaraman and Romanov lambs.

Article Snippet: The sections were incubated at 4 °C overnight with mouse monoclonal TLR4 (Novus Biologicals, CO, USA, NB100-56566, 1:100 dilution), rabbit polyclonal MyD88 (Novus Biologicals, CO, USA, NB100-56598, 1:100 dilution), and mouse monoclonal TNF-α primary antibodies (Bio-Rad Laboratories MCA905GA, 1:75 dilution).

Techniques: Gene Expression, Control