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traf-2 antibody  (Bio-Techne corporation)


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    Bio-Techne corporation traf-2 antibody
    Traf 2 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/traf-2 antibody/product/Bio-Techne corporation
    Average 94 stars, based on 4 article reviews
    traf-2 antibody - by Bioz Stars, 2026-05
    94/100 stars

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    Local effects of TNF on humanin, PCNA, SOX9 and <t>TRAF2</t> expressions in growth plates tissue specimens (n=3) in patient 1 and patient 2 analyzed separately. Quantitative analysis of (A, E) humanin staining, (B, F) PCNA staining, (C, G) SOX9 staining, and (D, H) TRAF2 staining. Error bars indicate mean ± SE. Students t-test was used to analyze differences between groups.
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    Figure 5: Immunostaining of HN30 and MCF-12a cells for <t>TRAF2</t> 3 days post-transfection. MCF-12a NT (DAPI): non- treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies.
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    Figure 5: Immunostaining of HN30 and MCF-12a cells for <t>TRAF2</t> 3 days post-transfection. MCF-12a NT (DAPI): non- treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies.
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    Figure 5: Immunostaining of HN30 and MCF-12a cells for <t>TRAF2</t> 3 days post-transfection. MCF-12a NT (DAPI): non- treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies.
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    Local effects of TNF on humanin, PCNA, SOX9 and TRAF2 expressions in growth plates tissue specimens (n=3) in patient 1 and patient 2 analyzed separately. Quantitative analysis of (A, E) humanin staining, (B, F) PCNA staining, (C, G) SOX9 staining, and (D, H) TRAF2 staining. Error bars indicate mean ± SE. Students t-test was used to analyze differences between groups.

    Journal: Frontiers in Endocrinology

    Article Title: A novel link between chronic inflammation and humanin regulation in children

    doi: 10.3389/fendo.2023.1142310

    Figure Lengend Snippet: Local effects of TNF on humanin, PCNA, SOX9 and TRAF2 expressions in growth plates tissue specimens (n=3) in patient 1 and patient 2 analyzed separately. Quantitative analysis of (A, E) humanin staining, (B, F) PCNA staining, (C, G) SOX9 staining, and (D, H) TRAF2 staining. Error bars indicate mean ± SE. Students t-test was used to analyze differences between groups.

    Article Snippet: Next, slides were incubated with anti-humanin antibody (NB100-56877; Novus Biologicals, Littleton, Colorado, USA), anti-proliferating cell nuclear antigen (PCNA) antibody (ab-18197; Abcam, Cambridge, United Kingdom), anti-SOX9 antibody (ab-5355; Sigma-Aldrich, Burlington, MA, USA), and anti-TRAF2 antibody (NB100-56173SS; Novus Biologicals, Littleton, Colorado, USA) overnight at 4°C, 1:300 diluted for all antibodies.

    Techniques: Staining

    TNF suppressed SOX9, PCNA and TRAF2 expressions in human growth plate tissue specimens (n=6) obtained from 2 children or human chondrocytes. (A, B) Quantitative analysis of SOX9 staining (yellow arrows), calculated as number of positive cells per mm². (C) Relative expression of SOX9 assessed by qPCR in HCS-2/8 cells treated for 72 hours with TNF at 10, 30, 100, 300 ng/ml concentrations (n=3). (D, E) Quantitative analysis of PCNA staining (yellow arrows), calculated as number of positive cells per mm². (F) Relative expression of PCNA assessed by qPCR in HCS-2/8 cells treated for 72 hours with TNF at 10, 30, 100, 300 ng/ml concentrations (n=3). (G) Western blot analysis of SOX9 and PCNA expressions in HCS-2/8 cells treated with TNF (100 ng/ml). (H, I) Quantification of SOX9 and PCNA expressions by three independent Western blot experiments. (J, K) Quantitative analysis of TRAF2 staining (yellow arrows), calculated as number of positive cells per mm². Error bars indicate mean ± SE. Students t-test was used to analyze differences between groups.

    Journal: Frontiers in Endocrinology

    Article Title: A novel link between chronic inflammation and humanin regulation in children

    doi: 10.3389/fendo.2023.1142310

    Figure Lengend Snippet: TNF suppressed SOX9, PCNA and TRAF2 expressions in human growth plate tissue specimens (n=6) obtained from 2 children or human chondrocytes. (A, B) Quantitative analysis of SOX9 staining (yellow arrows), calculated as number of positive cells per mm². (C) Relative expression of SOX9 assessed by qPCR in HCS-2/8 cells treated for 72 hours with TNF at 10, 30, 100, 300 ng/ml concentrations (n=3). (D, E) Quantitative analysis of PCNA staining (yellow arrows), calculated as number of positive cells per mm². (F) Relative expression of PCNA assessed by qPCR in HCS-2/8 cells treated for 72 hours with TNF at 10, 30, 100, 300 ng/ml concentrations (n=3). (G) Western blot analysis of SOX9 and PCNA expressions in HCS-2/8 cells treated with TNF (100 ng/ml). (H, I) Quantification of SOX9 and PCNA expressions by three independent Western blot experiments. (J, K) Quantitative analysis of TRAF2 staining (yellow arrows), calculated as number of positive cells per mm². Error bars indicate mean ± SE. Students t-test was used to analyze differences between groups.

    Article Snippet: Next, slides were incubated with anti-humanin antibody (NB100-56877; Novus Biologicals, Littleton, Colorado, USA), anti-proliferating cell nuclear antigen (PCNA) antibody (ab-18197; Abcam, Cambridge, United Kingdom), anti-SOX9 antibody (ab-5355; Sigma-Aldrich, Burlington, MA, USA), and anti-TRAF2 antibody (NB100-56173SS; Novus Biologicals, Littleton, Colorado, USA) overnight at 4°C, 1:300 diluted for all antibodies.

    Techniques: Staining, Expressing, Western Blot

    Figure 5: Immunostaining of HN30 and MCF-12a cells for TRAF2 3 days post-transfection. MCF-12a NT (DAPI): non- treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies.

    Journal: Oncotarget

    Article Title: A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma.

    doi: 10.18632/oncotarget.306

    Figure Lengend Snippet: Figure 5: Immunostaining of HN30 and MCF-12a cells for TRAF2 3 days post-transfection. MCF-12a NT (DAPI): non- treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies.

    Article Snippet: Next day, membranes were cut and incubated with primary rabbit polyclonal NSP5a3a (Novus NB100-517A) at 1/500, primary rabbit polyclonal p73 ( Santa Cruz H-79) at 1/200, primary goat polyclonal p73 (Santa Cruz S-20) at 1/200, primary mouse monoclonal alpha-tubulin (Invitrogen) at 1/5000, primary rabbit polyclonal TRADD (Cell Signaling) 1/1000, primary rabbit polyclonal TRAF2 (Novus NB10056173) at 1/1000, primary rabbit polyclonal DAXX (Cell Signaling 25C12) at 1/1000, primary rabbit polyclonal cleaved caspase 7 (Cell Signaling D198) at 1/x, primary rabbit polyclonal cleaved caspase 8 (Cell Signaling 18C8) at 1/x, rabbit primary polyclonal cleaved caspase 9 (Cell Signaling D3C5) at 1/x, primary rabbit polyclonal caspase 1 (Cell Signaling) at 1/x or primary mouse monoclonal B23 (Sigma) at 1/500 in 5% milk buffer TTBS for 2 hours at RT.

    Techniques: Immunostaining, Transfection, Staining

    Figure 10: Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated and HN30 cells transfected with pcDNA 3.0 NSP 5a3a and pcDNA 3.1/CT-GFP for 3 days. NT: non-treated HN30 cells, TRD: HN30 cells over-expressing NSP 5a3a. a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-DAXX., b. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a and western blot anaylsis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti- TRAF2., d. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-TRAF2., e. Immunoprecipitation of non-treated HN30 cells using rabbit anti-TRAF2/DAXX and western blot analysis using rabbit anti-NSP 5a3a, and f. Immunoprecipitation of HN30 treated cells using rabbit anti-TRAF2/DAXX and western blot analysis using rabbit anti-NSP 5a3a.

    Journal: Oncotarget

    Article Title: A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma.

    doi: 10.18632/oncotarget.306

    Figure Lengend Snippet: Figure 10: Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated and HN30 cells transfected with pcDNA 3.0 NSP 5a3a and pcDNA 3.1/CT-GFP for 3 days. NT: non-treated HN30 cells, TRD: HN30 cells over-expressing NSP 5a3a. a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-DAXX., b. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a and western blot anaylsis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti- TRAF2., d. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-TRAF2., e. Immunoprecipitation of non-treated HN30 cells using rabbit anti-TRAF2/DAXX and western blot analysis using rabbit anti-NSP 5a3a, and f. Immunoprecipitation of HN30 treated cells using rabbit anti-TRAF2/DAXX and western blot analysis using rabbit anti-NSP 5a3a.

    Article Snippet: Next day, membranes were cut and incubated with primary rabbit polyclonal NSP5a3a (Novus NB100-517A) at 1/500, primary rabbit polyclonal p73 ( Santa Cruz H-79) at 1/200, primary goat polyclonal p73 (Santa Cruz S-20) at 1/200, primary mouse monoclonal alpha-tubulin (Invitrogen) at 1/5000, primary rabbit polyclonal TRADD (Cell Signaling) 1/1000, primary rabbit polyclonal TRAF2 (Novus NB10056173) at 1/1000, primary rabbit polyclonal DAXX (Cell Signaling 25C12) at 1/1000, primary rabbit polyclonal cleaved caspase 7 (Cell Signaling D198) at 1/x, primary rabbit polyclonal cleaved caspase 8 (Cell Signaling 18C8) at 1/x, rabbit primary polyclonal cleaved caspase 9 (Cell Signaling D3C5) at 1/x, primary rabbit polyclonal caspase 1 (Cell Signaling) at 1/x or primary mouse monoclonal B23 (Sigma) at 1/500 in 5% milk buffer TTBS for 2 hours at RT.

    Techniques: Western Blot, Transfection, Expressing, Immunoprecipitation

    Figure 5: Immunostaining of HN30 and MCF-12a cells for TRAF2 3 days post-transfection. MCF-12a NT (DAPI): non- treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies.

    Journal: Oncotarget

    Article Title: A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma.

    doi: 10.18632/oncotarget.306

    Figure Lengend Snippet: Figure 5: Immunostaining of HN30 and MCF-12a cells for TRAF2 3 days post-transfection. MCF-12a NT (DAPI): non- treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies.

    Article Snippet: Cells were pre-blocked for 1 hour at room temperature with 8% BSA in PBS 1x, then cells were incubated with either with rabbit polyclonal NSP 5a3a (Novus NB100-517A) at 1/500, rabbit polyclonal TRADD (Cell Signaling) at 1/1000, rabbit polyclonal TRAF2 (Novus NB100-56173) at 1/1000, or rabbit polyclonal p73 (Santa Cruz H-79) at 1/200 dilution in 1% BSA overnight at 4oC.

    Techniques: Immunostaining, Transfection, Staining

    Figure 10: Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated and HN30 cells transfected with pcDNA 3.0 NSP 5a3a and pcDNA 3.1/CT-GFP for 3 days. NT: non-treated HN30 cells, TRD: HN30 cells over-expressing NSP 5a3a. a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-DAXX., b. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a and western blot anaylsis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti- TRAF2., d. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-TRAF2., e. Immunoprecipitation of non-treated HN30 cells using rabbit anti-TRAF2/DAXX and western blot analysis using rabbit anti-NSP 5a3a, and f. Immunoprecipitation of HN30 treated cells using rabbit anti-TRAF2/DAXX and western blot analysis using rabbit anti-NSP 5a3a.

    Journal: Oncotarget

    Article Title: A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma.

    doi: 10.18632/oncotarget.306

    Figure Lengend Snippet: Figure 10: Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated and HN30 cells transfected with pcDNA 3.0 NSP 5a3a and pcDNA 3.1/CT-GFP for 3 days. NT: non-treated HN30 cells, TRD: HN30 cells over-expressing NSP 5a3a. a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-DAXX., b. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a and western blot anaylsis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti- TRAF2., d. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-TRAF2., e. Immunoprecipitation of non-treated HN30 cells using rabbit anti-TRAF2/DAXX and western blot analysis using rabbit anti-NSP 5a3a, and f. Immunoprecipitation of HN30 treated cells using rabbit anti-TRAF2/DAXX and western blot analysis using rabbit anti-NSP 5a3a.

    Article Snippet: Cells were pre-blocked for 1 hour at room temperature with 8% BSA in PBS 1x, then cells were incubated with either with rabbit polyclonal NSP 5a3a (Novus NB100-517A) at 1/500, rabbit polyclonal TRADD (Cell Signaling) at 1/1000, rabbit polyclonal TRAF2 (Novus NB100-56173) at 1/1000, or rabbit polyclonal p73 (Santa Cruz H-79) at 1/200 dilution in 1% BSA overnight at 4oC.

    Techniques: Western Blot, Transfection, Expressing, Immunoprecipitation

    Figure 5: Immunostaining of HN30 and MCF-12a cells for TRAF2 3 days post-transfection. MCF-12a NT (DAPI): non- treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies.

    Journal: Oncotarget

    Article Title: A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma.

    doi: 10.18632/oncotarget.306

    Figure Lengend Snippet: Figure 5: Immunostaining of HN30 and MCF-12a cells for TRAF2 3 days post-transfection. MCF-12a NT (DAPI): non- treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies.

    Article Snippet: We can expect to see a broad range of isoforms since we used a rabbit polyclonal TRAF2 (Novus NB100-56173) antibody that has been documented to be able to detect a broad range of TRAF2 isoforms again depending on cell type and conditions.

    Techniques: Immunostaining, Transfection, Staining

    Figure 10: Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated and HN30 cells transfected with pcDNA 3.0 NSP 5a3a and pcDNA 3.1/CT-GFP for 3 days. NT: non-treated HN30 cells, TRD: HN30 cells over-expressing NSP 5a3a. a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-DAXX., b. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a and western blot anaylsis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti- TRAF2., d. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-TRAF2., e. Immunoprecipitation of non-treated HN30 cells using rabbit anti-TRAF2/DAXX and western blot analysis using rabbit anti-NSP 5a3a, and f. Immunoprecipitation of HN30 treated cells using rabbit anti-TRAF2/DAXX and western blot analysis using rabbit anti-NSP 5a3a.

    Journal: Oncotarget

    Article Title: A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma.

    doi: 10.18632/oncotarget.306

    Figure Lengend Snippet: Figure 10: Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated and HN30 cells transfected with pcDNA 3.0 NSP 5a3a and pcDNA 3.1/CT-GFP for 3 days. NT: non-treated HN30 cells, TRD: HN30 cells over-expressing NSP 5a3a. a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-DAXX., b. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a and western blot anaylsis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti- TRAF2., d. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-TRAF2., e. Immunoprecipitation of non-treated HN30 cells using rabbit anti-TRAF2/DAXX and western blot analysis using rabbit anti-NSP 5a3a, and f. Immunoprecipitation of HN30 treated cells using rabbit anti-TRAF2/DAXX and western blot analysis using rabbit anti-NSP 5a3a.

    Article Snippet: We can expect to see a broad range of isoforms since we used a rabbit polyclonal TRAF2 (Novus NB100-56173) antibody that has been documented to be able to detect a broad range of TRAF2 isoforms again depending on cell type and conditions.

    Techniques: Western Blot, Transfection, Expressing, Immunoprecipitation