anti gapdh  (Bio-Techne corporation)

Journal: Frontiers in Oncology

Article Title: Human Endogenous Retroviruses Long Terminal Repeat Methylation, Transcription, and Protein Expression in Human Colon Cancer

doi: 10.3389/fonc.2020.569015

Figure Lengend Snippet: (A–G) HERV-K Pol protein expression in colon cancer and normal tissues retrieved from negative surgical margins. HERV-K Pol protein was mainly expressed as three different complexes: polymerase intermediates (Pol, 100 kDa), reverse transcriptase-RNase H (RT-RH, 60 kDa), and reverse transcriptase (RT, 54 kDa). (H) HERV-K Env protein (ERVK-7 Env) expression in colon cancer and normal tissues retrieved from negative surgical margins of one patient. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: Overnight incubation with the following primary antibodies was performed: anti-GAPDH (Bio-Techne, United States) diluted 1:7000, anti-ERVK-2 Pol (Novus Biological, United States) diluted 1:1000, and anti-ERVK-7 Env Polyclonal Antibody (Thermo Fisher Scientific, United States) diluted 1:1000.

Techniques: Expressing

Journal: Arthritis Research & Therapy

Article Title: Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis

doi: 10.1186/s13075-018-1592-1

Figure Lengend Snippet: Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets show high-power magnification of co-localisation. Representative images show single immunofluorescence of 4-HNE, VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B along with their controls. Isotype-matched antibodies are shown in Additional file : Figure S3 and Additional file : Figure S4

Article Snippet: The sections were incubated with rabbit monoclonal primary antibodies against human VEGF, Ang2, Tie2, ATP5B (all from Abcam), GAPDH (Trevigen) and mouse monoclonal antibodies against human 4-HNE (GENTAUR).

Techniques: Double Immunofluorescence Staining, Incubation, Marker, Immunofluorescence, Staining