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anti-bik ab nb100–56109  (Novus Biologicals)
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Novus Biologicals anti-bik ab nb100–56109
Effect of MTX on expression of circadian transcriptional factors PAR bZIP and <t>Bik</t> in synovial fibroblasts. a Cells treated with MTX (10, 100 nM) or control media for 24 h to measure expression of PAR bZIP gene mRNA ( Dbp, Tef, and Hlf ) and E4bp4 . Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on boxplots using black symbols. * p < 0.05 vs control, n = 5. b Cells treated with MTX (10, 100 nM) or control media for 24 h to measure expression of Bik gene mRNA. Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on boxplots using black symbols. * p < 0.05 vs control, n = 5. c Cells treated with MTX (10, 100 nM) or control media for 48 h to <t>observe</t> <t>CYTOCHROME</t> C expression. d Cells treated with MTX (10, 100 nM) or control media for 48 h to measure CYTOCHROME C expression by ELISA. Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on boxplots using black symbols. * p < 0.05 vs control, n = 5. Bik Bcl-2 interacting killer, Dbp D site of the albumin promoter binding protein, E4bp4 E4-binding protein 4, Hlf hepatic leukemia factor, mRNA messenger RNA, MTX methotrexate, Tef thyrotroph embryonic factor
Anti Bik Ab Nb100–56109, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of MTX on expression of circadian transcriptional factors PAR bZIP and Bik in synovial fibroblasts. a Cells treated with MTX (10, 100 nM) or control media for 24 h to measure expression of PAR bZIP gene mRNA ( Dbp, Tef, and Hlf ) and E4bp4 . Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on boxplots using black symbols. * p < 0.05 vs control, n = 5. b Cells treated with MTX (10, 100 nM) or control media for 24 h to measure expression of Bik gene mRNA. Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on boxplots using black symbols. * p < 0.05 vs control, n = 5. c Cells treated with MTX (10, 100 nM) or control media for 48 h to observe CYTOCHROME C expression. d Cells treated with MTX (10, 100 nM) or control media for 48 h to measure CYTOCHROME C expression by ELISA. Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on boxplots using black symbols. * p < 0.05 vs control, n = 5. Bik Bcl-2 interacting killer, Dbp D site of the albumin promoter binding protein, E4bp4 E4-binding protein 4, Hlf hepatic leukemia factor, mRNA messenger RNA, MTX methotrexate, Tef thyrotroph embryonic factor

Journal: Arthritis Research & Therapy

Article Title: Methotrexate upregulates circadian transcriptional factors PAR bZIP to induce apoptosis on rheumatoid arthritis synovial fibroblasts

doi: 10.1186/s13075-018-1552-9

Figure Lengend Snippet: Effect of MTX on expression of circadian transcriptional factors PAR bZIP and Bik in synovial fibroblasts. a Cells treated with MTX (10, 100 nM) or control media for 24 h to measure expression of PAR bZIP gene mRNA ( Dbp, Tef, and Hlf ) and E4bp4 . Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on boxplots using black symbols. * p < 0.05 vs control, n = 5. b Cells treated with MTX (10, 100 nM) or control media for 24 h to measure expression of Bik gene mRNA. Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on boxplots using black symbols. * p < 0.05 vs control, n = 5. c Cells treated with MTX (10, 100 nM) or control media for 48 h to observe CYTOCHROME C expression. d Cells treated with MTX (10, 100 nM) or control media for 48 h to measure CYTOCHROME C expression by ELISA. Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on boxplots using black symbols. * p < 0.05 vs control, n = 5. Bik Bcl-2 interacting killer, Dbp D site of the albumin promoter binding protein, E4bp4 E4-binding protein 4, Hlf hepatic leukemia factor, mRNA messenger RNA, MTX methotrexate, Tef thyrotroph embryonic factor

Article Snippet: Antibodies (Abs) used were: anti-PER2 Ab (sc-101,105; Santa Cruz, Dallas, TX, USA), anti-CYTOCHROME C Ab (ab13575; Abcam, Cambridge, UK), anti-BIK Ab (NB100–56109; Novus, Littleton, CO, USA), anti-Actin Ab (sc-1616; Santa Cruz), anti-mouse IgG Ab (NA9310; GE Healthcare, Chicago, IL, USA), and anti-rabbit IgG Ab (NA9340; GE Healthcare).

Techniques: Expressing, Control, Enzyme-linked Immunosorbent Assay, Binding Assay

PER2, BIK, and CYTOCHROME C expression and morphological changes of the nucleus. a Cells stained with DAPI and anti-PER2 Ab after treatment with MTX (10, 100 nM) or control media for 24 h. Arrowheads indicate apoptotic cells. b Cells stained with DAPI, anti-BIK Ab, and anti-CYTOCHROME C Ab after treatment with MTX (10, 100 nM) or control media for 24 h. Arrowheads indicate apoptotic cells. Bik Bcl-2 interacting killer, DAPI 4′,6-diamidino-2-phenylindole, MTX methotrexate, Per period

Journal: Arthritis Research & Therapy

Article Title: Methotrexate upregulates circadian transcriptional factors PAR bZIP to induce apoptosis on rheumatoid arthritis synovial fibroblasts

doi: 10.1186/s13075-018-1552-9

Figure Lengend Snippet: PER2, BIK, and CYTOCHROME C expression and morphological changes of the nucleus. a Cells stained with DAPI and anti-PER2 Ab after treatment with MTX (10, 100 nM) or control media for 24 h. Arrowheads indicate apoptotic cells. b Cells stained with DAPI, anti-BIK Ab, and anti-CYTOCHROME C Ab after treatment with MTX (10, 100 nM) or control media for 24 h. Arrowheads indicate apoptotic cells. Bik Bcl-2 interacting killer, DAPI 4′,6-diamidino-2-phenylindole, MTX methotrexate, Per period

Article Snippet: Antibodies (Abs) used were: anti-PER2 Ab (sc-101,105; Santa Cruz, Dallas, TX, USA), anti-CYTOCHROME C Ab (ab13575; Abcam, Cambridge, UK), anti-BIK Ab (NB100–56109; Novus, Littleton, CO, USA), anti-Actin Ab (sc-1616; Santa Cruz), anti-mouse IgG Ab (NA9310; GE Healthcare, Chicago, IL, USA), and anti-rabbit IgG Ab (NA9340; GE Healthcare).

Techniques: Expressing, Staining, Control