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bag5 antibody  (Bio-Techne corporation)


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    Bio-Techne corporation bag5 antibody
    Bag5 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bag5 antibody/product/Bio-Techne corporation
    Average 90 stars, based on 3 article reviews
    bag5 antibody - by Bioz Stars, 2026-04
    90/100 stars

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    Chronic MPP+ treatment induces BAG6 expression. Differentiated SH-SY5Y cells were either untreated (Ctrl) or treated with 250 μm of MPP+ for 2 weeks, and protein expression was analyzed. A, chronic MPP+ treatment induces BAG6 expression and decreases BAG2, <t>BAG5,</t> and PINK1 levels. B, BAG5 protein quantification. *, p = 0.0005. C, BAG2 protein quantification. *, p < 0.0001. D, BAG6 protein quantification. *, p < 0.0001; see also Fig. 1F. E, PINK1-FL. *, p < 0.0001 and PINK1-dN protein quantification, *, p < 0.0001. F, BAG6 mRNA expression normalized to GAPDH, p = 0.0037. Graphs represent means ± S.D.; n = 5 independent experiments for B–E; n = 4 independent experiments for F; Student's two-tailed t test.
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    List of antibodies used in the study

    Journal: The Journal of Biological Chemistry

    Article Title: Chronic treatment with the complex I inhibitor MPP + depletes endogenous PTEN-induced kinase 1 (PINK1) via up-regulation of Bcl-2–associated athanogene 6 (BAG6)

    doi: 10.1074/jbc.RA119.010474

    Figure Lengend Snippet: List of antibodies used in the study

    Article Snippet: BAG5 , Rabbit , NB100-56091 , Novus Biological , 1:1000.

    Techniques: Transduction

    Chronic MPP+ treatment induces BAG6 expression. Differentiated SH-SY5Y cells were either untreated (Ctrl) or treated with 250 μm of MPP+ for 2 weeks, and protein expression was analyzed. A, chronic MPP+ treatment induces BAG6 expression and decreases BAG2, BAG5, and PINK1 levels. B, BAG5 protein quantification. *, p = 0.0005. C, BAG2 protein quantification. *, p < 0.0001. D, BAG6 protein quantification. *, p < 0.0001; see also Fig. 1F. E, PINK1-FL. *, p < 0.0001 and PINK1-dN protein quantification, *, p < 0.0001. F, BAG6 mRNA expression normalized to GAPDH, p = 0.0037. Graphs represent means ± S.D.; n = 5 independent experiments for B–E; n = 4 independent experiments for F; Student's two-tailed t test.

    Journal: The Journal of Biological Chemistry

    Article Title: Chronic treatment with the complex I inhibitor MPP + depletes endogenous PTEN-induced kinase 1 (PINK1) via up-regulation of Bcl-2–associated athanogene 6 (BAG6)

    doi: 10.1074/jbc.RA119.010474

    Figure Lengend Snippet: Chronic MPP+ treatment induces BAG6 expression. Differentiated SH-SY5Y cells were either untreated (Ctrl) or treated with 250 μm of MPP+ for 2 weeks, and protein expression was analyzed. A, chronic MPP+ treatment induces BAG6 expression and decreases BAG2, BAG5, and PINK1 levels. B, BAG5 protein quantification. *, p = 0.0005. C, BAG2 protein quantification. *, p < 0.0001. D, BAG6 protein quantification. *, p < 0.0001; see also Fig. 1F. E, PINK1-FL. *, p < 0.0001 and PINK1-dN protein quantification, *, p < 0.0001. F, BAG6 mRNA expression normalized to GAPDH, p = 0.0037. Graphs represent means ± S.D.; n = 5 independent experiments for B–E; n = 4 independent experiments for F; Student's two-tailed t test.

    Article Snippet: Densitometry data were normalized to loading control, and the half-lives of PINK1 isoforms were estimated by fitting a one-phase exponential decay curve using Prism 8 software (GraphPad, San Diego, CA). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Species Catalog number Source Dilution PINK1 Rabbit BC100-494 Novus Biological 1:1000 TOM20 Rabbit Sc11415 Santa Cruz 1:10,000 β-ACTIN Mouse A5316 Sigma 1:2000 HSP60 Mouse 611563 BD Transduction 1:1000 BAG6 Mouse Sc365928 Santa Cruz 1:1000 BAG5 Rabbit NB100-56091 Novus Biological 1:1000 BAG2 Mouse Sc101216 Santa Cruz 1:1000 HSP90 Rabbit 4877 Cell Signaling 1:1000 CDC37 Rabbit 3604 Cell Signaling 1:1000 CHIP Rabbit Sc66830 Santa Cruz 1:1000 GAPDH Rabbit Ab37168 Abcam 1:10,000 GFP Rabbit A6455 Invitrogen 1:10,000 V5 Mouse Sc271944 Santa Cruz 1:1000 FLAG Rabbit F7425 Sigma 1:1000 β-Tubulin Rabbit Ab6046 Abcam 1:5000 Open in a separate window List of antibodies used in the study

    Techniques: Expressing, Two Tailed Test

    List of antibodies used in the study

    Journal: The Journal of Biological Chemistry

    Article Title: Chronic treatment with the complex I inhibitor MPP + depletes endogenous PTEN-induced kinase 1 (PINK1) via up-regulation of Bcl-2–associated athanogene 6 (BAG6)

    doi: 10.1074/jbc.RA119.010474

    Figure Lengend Snippet: List of antibodies used in the study

    Article Snippet: Densitometry data were normalized to loading control, and the half-lives of PINK1 isoforms were estimated by fitting a one-phase exponential decay curve using Prism 8 software (GraphPad, San Diego, CA). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Species Catalog number Source Dilution PINK1 Rabbit BC100-494 Novus Biological 1:1000 TOM20 Rabbit Sc11415 Santa Cruz 1:10,000 β-ACTIN Mouse A5316 Sigma 1:2000 HSP60 Mouse 611563 BD Transduction 1:1000 BAG6 Mouse Sc365928 Santa Cruz 1:1000 BAG5 Rabbit NB100-56091 Novus Biological 1:1000 BAG2 Mouse Sc101216 Santa Cruz 1:1000 HSP90 Rabbit 4877 Cell Signaling 1:1000 CDC37 Rabbit 3604 Cell Signaling 1:1000 CHIP Rabbit Sc66830 Santa Cruz 1:1000 GAPDH Rabbit Ab37168 Abcam 1:10,000 GFP Rabbit A6455 Invitrogen 1:10,000 V5 Mouse Sc271944 Santa Cruz 1:1000 FLAG Rabbit F7425 Sigma 1:1000 β-Tubulin Rabbit Ab6046 Abcam 1:5000 Open in a separate window List of antibodies used in the study

    Techniques: Transduction