tlr4  (Bio-Techne corporation)

Journal: Scientific Reports

Article Title: 7,8-Dihydroxyflavone improves neuropathological changes in the brain of Tg26 mice, a model for HIV-associated neurocognitive disorder

doi: 10.1038/s41598-021-97220-8

Figure Lengend Snippet: List of primary antibodies.

Article Snippet: Anti TLR4 , Toll-like receptor 4 , Novus Biotechne , 1:200 (IHC).

Techniques: Concentration Assay, Marker

Journal: Scientific Reports

Article Title: 7,8-Dihydroxyflavone improves neuropathological changes in the brain of Tg26 mice, a model for HIV-associated neurocognitive disorder

doi: 10.1038/s41598-021-97220-8

Figure Lengend Snippet: DHF treatment downregulates activation of the TLR4 and NFkB. Legend: TLR4 and NFkB expression in the hippocampus and cortex of Wild Type (WT), Tg26, and DHF treated Tg26 mice ( A - P ). Immunohistochemical stained sections show TLR4 and NFkB expressing cells in the hippocampal and cortex regions of the mice brains. 3 and 3 WT mice, respectively, 3 Tg26 mice, and 4 TG + DHF mice were used. A, B, C, E, F, G, I, J, K, M, N, and O are 400 × magnification pictures of the hippocampus and cortex regions of the mice. ( D ) Quantification of TLR4 expressing cells in the hippocampus: p < 0.001, WT versus Tg; p < 0.001 Tg versus Tg + DHF. ( H ) Quantification of TLR4 expressing cells in cortex: p < 0.05, WT versus Tg; p < 0.001, Tg versus Tg + DHF. ( L ) Quantification of NFkB expressing cells in the hippocampus: p < 0.001, WT versus Tg; p < 0.001 Tg versus Tg + DHF. ( P ) Quantification of NFkB expressing cells in cortex: p < 0.001, WT versus Tg; p > 0.05, Tg versus Tg + DHF. The total numbers of hippocampal fields analyzed (WT, Tg26, TG + DHF) for each antibody were TLR4 (55, 87, 104), NFkB (154, 72, 101). The total numbers of cortex fields analyzed (WT, Tg26, TG + DHF) for each antibody were TLR4 (15, 15, 20), NFkB (15, 15, 20). One Way ANOVA with Bonferroni's Multiple Comparison post- test. scale bar, 100 μm.

Article Snippet: Anti TLR4 , Toll-like receptor 4 , Novus Biotechne , 1:200 (IHC).

Techniques: Activation Assay, Expressing, Immunohistochemical staining, Staining

Journal: Scientific Reports

Article Title: A role for intestinal TLR4-driven inflammatory response during activity-based anorexia

doi: 10.1038/srep35813

Figure Lengend Snippet: ( A ) TLR4 mRNA levels in colonic mucosa of CT, ABA and LFA mice at d10 and d17. Data are shown as mean ± SEM. Results were normalized to the values in GAPDH control amplifications. *p < 0.05 vs CT; **p = < 0.005 vs CT. ( B ) TLR4 expression protein assessed in colonic samples from CT, ABA and LFA mice.*p < 0.0001 vs CT. ( C ) TLR4–MD-2 expression on epithelial cells (EpCam + cells) isolated from colonic mucosa. MFI, mean fluorescence intensity. *p < 0.001 vs CT; φ p < 0.05 vs d10. ( D ) TLR4–MD-2 expression on macrophages isolated from colonic mucosa. *p < 0.05 vs CT. ( E ) Representative flow cytometry analysis of colonic epithelial cells (EpCam + ) cells and representative histograms for TLR4 of the outlined population for CT, ABA and LFA.

Article Snippet: Then, an overnight incubation at 4 °C was done with mouse anti- TLR4 (working dilution = 1:1000; Novus biologicals, Littleton, CO), PPAR-γ (working dilution = 1:1,000; Fisher Scientific, Illkirch, France), IL-1β, MyD88, NfκB p65 and IRAK-4 (working dilution = 1:1000, Santa Cruz Biotechnology, Tebu-Bio, Le Perray en Yvelines, France).

Techniques: Control, Expressing, Isolation, Fluorescence, Flow Cytometry

Journal: Scientific Reports

Article Title: A role for intestinal TLR4-driven inflammatory response during activity-based anorexia

doi: 10.1038/srep35813

Figure Lengend Snippet: Representative photomicrographs show the distribution of TLR4 protein in the colonic mucosa of ABA, LFA and CT. Green, TLR4 staining; blue, DAPI nuclear staining. Original magnification, ×20.

Article Snippet: Then, an overnight incubation at 4 °C was done with mouse anti- TLR4 (working dilution = 1:1000; Novus biologicals, Littleton, CO), PPAR-γ (working dilution = 1:1,000; Fisher Scientific, Illkirch, France), IL-1β, MyD88, NfκB p65 and IRAK-4 (working dilution = 1:1000, Santa Cruz Biotechnology, Tebu-Bio, Le Perray en Yvelines, France).

Techniques: Staining

Journal: Scientific Reports

Article Title: A role for intestinal TLR4-driven inflammatory response during activity-based anorexia

doi: 10.1038/srep35813

Figure Lengend Snippet: ( A ) TLR4 protein expression and ( B ) MyD88 protein expression in the hypothalamus of CT, ABA and LFA mice at d10 and d17, ( C ) TRIF mRNA expression and ( D ) TRAM mRNA expression in the hypothalamus of CT, ABA and LFA mice at d17.

Article Snippet: Then, an overnight incubation at 4 °C was done with mouse anti- TLR4 (working dilution = 1:1000; Novus biologicals, Littleton, CO), PPAR-γ (working dilution = 1:1,000; Fisher Scientific, Illkirch, France), IL-1β, MyD88, NfκB p65 and IRAK-4 (working dilution = 1:1000, Santa Cruz Biotechnology, Tebu-Bio, Le Perray en Yvelines, France).

Techniques: Expressing

Journal: Scientific Reports

Article Title: A role for intestinal TLR4-driven inflammatory response during activity-based anorexia

doi: 10.1038/srep35813

Figure Lengend Snippet: ( A ) IL-1β protein expression, ( D ) IL-1R1 protein expression and ( E ) IRAK-4 protein expression in the hypothalamus of CT, ABA and LFA mice at d17. *p < 0.05 vs CT, **p < 0.01 vs CT. ( F ) Representative immunoblots for TLR4, MyD88, IRAK-4, IL1-R1 and GAPDH from CT, ABA and LFA mice at d17.

Article Snippet: Then, an overnight incubation at 4 °C was done with mouse anti- TLR4 (working dilution = 1:1000; Novus biologicals, Littleton, CO), PPAR-γ (working dilution = 1:1,000; Fisher Scientific, Illkirch, France), IL-1β, MyD88, NfκB p65 and IRAK-4 (working dilution = 1:1000, Santa Cruz Biotechnology, Tebu-Bio, Le Perray en Yvelines, France).

Techniques: Expressing, Western Blot

Journal: Scientific Reports

Article Title: A role for intestinal TLR4-driven inflammatory response during activity-based anorexia

doi: 10.1038/srep35813

Figure Lengend Snippet: ( A ) Survival curves for CT (n = 6), ABA wt (n = 6) and ABA TLR4 −/− (n = 6) C57BL/6 female mice, *log-rank test, p = 0.0057. ( B ) Food intake and ( C ) Percentage of body weight change measured in control mice (CT, closed circles) and in ABA wild-type (ABA wt, open squares) and ABA TLR4 −/− , open circles) mice placed in cages with activity wheel and limitation of food access. ( B ) ***p < 0.001 vs ABA wt and ABA TLR4 −/− , a, p < 0.05. ( C ) The data represents cumulative body weight loss expressed in % / d6. ***p < 0.0001 vs ABA wt and ABA TLR4 −/− . Wheel activity measured at d5 ( D ) and at d11 ( E ), expressed in cm, measured in ABA wt (closed circles) and ABA TLR4 −/− (open circles) mice by using RunningWheel® software (Intellibio, France). Activities during the dark or light phases were continuously monitored. *p < 0.05; **p < 0.01; ***p < 0.001. Two-way ANOVA and Bonferroni post-test were used for B, C, D and E.

Article Snippet: Then, an overnight incubation at 4 °C was done with mouse anti- TLR4 (working dilution = 1:1000; Novus biologicals, Littleton, CO), PPAR-γ (working dilution = 1:1,000; Fisher Scientific, Illkirch, France), IL-1β, MyD88, NfκB p65 and IRAK-4 (working dilution = 1:1000, Santa Cruz Biotechnology, Tebu-Bio, Le Perray en Yvelines, France).

Techniques: Control, Activity Assay, Software

Journal: Scientific Reports

Article Title: A role for intestinal TLR4-driven inflammatory response during activity-based anorexia

doi: 10.1038/srep35813

Figure Lengend Snippet: Sequences of primers used for PCR.

Article Snippet: Then, an overnight incubation at 4 °C was done with mouse anti- TLR4 (working dilution = 1:1000; Novus biologicals, Littleton, CO), PPAR-γ (working dilution = 1:1,000; Fisher Scientific, Illkirch, France), IL-1β, MyD88, NfκB p65 and IRAK-4 (working dilution = 1:1000, Santa Cruz Biotechnology, Tebu-Bio, Le Perray en Yvelines, France).

Techniques: