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rabbit polyclonal anti nsp 5a3a  (Novus Biologicals)
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Novus Biologicals rabbit polyclonal anti nsp 5a3a
Figure 1. Image of silver stained gel and protein bands identified by proteomic analysis. Lane 1. Immunoprecipitation of MCF-12A total lysates using anti-B23. Lane 2. Immunoprecipitation of MCF-12A total lysates using anti-NSP <t>5a3a.</t>
Rabbit Polyclonal Anti Nsp 5a3a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. Image of silver stained gel and protein bands identified by proteomic analysis. Lane 1. Immunoprecipitation of MCF-12A total lysates using anti-B23. Lane 2. Immunoprecipitation of MCF-12A total lysates using anti-NSP 5a3a.

Journal: Cell cycle (Georgetown, Tex.)

Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

doi: 10.4161/cc.9.6.10991

Figure Lengend Snippet: Figure 1. Image of silver stained gel and protein bands identified by proteomic analysis. Lane 1. Immunoprecipitation of MCF-12A total lysates using anti-B23. Lane 2. Immunoprecipitation of MCF-12A total lysates using anti-NSP 5a3a.

Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with rabbit polyclonal anti-NSP 5a3a (Novus) at 1/500 dilution in 1% BSA over night at 4°C.

Techniques: Staining, Immunoprecipitation

Figure 3. Subcellular localization of NSP 5a3a in MCF-7 cells by immunostaining. MCF-7 cells were stained with rabbit anti-NSP5a3a then with anti- rabbit Tritc secondary. Subsequently, MCF-7 cells were stained with mouse anti-B23 then with anti-rabbit Fitc secondary to correlate how NSP 5a3a localizes with respect to B23. Controls consisted of MCF-12A cells stained with anti-rabbit Tritc secondary only and anti-rabbit Fitc secondary only. Nuc (nucleus): nuc (nucleoli).

Journal: Cell cycle (Georgetown, Tex.)

Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

doi: 10.4161/cc.9.6.10991

Figure Lengend Snippet: Figure 3. Subcellular localization of NSP 5a3a in MCF-7 cells by immunostaining. MCF-7 cells were stained with rabbit anti-NSP5a3a then with anti- rabbit Tritc secondary. Subsequently, MCF-7 cells were stained with mouse anti-B23 then with anti-rabbit Fitc secondary to correlate how NSP 5a3a localizes with respect to B23. Controls consisted of MCF-12A cells stained with anti-rabbit Tritc secondary only and anti-rabbit Fitc secondary only. Nuc (nucleus): nuc (nucleoli).

Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with rabbit polyclonal anti-NSP 5a3a (Novus) at 1/500 dilution in 1% BSA over night at 4°C.

Techniques: Immunostaining, Staining

Figure 2. Subcellular localization of NSP 5a3a in MCF-12A cells by immunostaining. MCF-12A cells were stained with rabbit anti-NSP5a3a then with anti-rabbit Tritc secondary. Subsequently, MCF-12a cells were stained with mouse anti-B23 then with anti-rabbit Fitc secondary to correlate how NSP 5a3a localizes with respect to B23. Controls consisted of MCF-12A cells stained with anti-rabbit Tritc secondary only and anti-rabbit Fitc secondary only. Nuc (nucleus): nuc (nucleoli).

Journal: Cell cycle (Georgetown, Tex.)

Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

doi: 10.4161/cc.9.6.10991

Figure Lengend Snippet: Figure 2. Subcellular localization of NSP 5a3a in MCF-12A cells by immunostaining. MCF-12A cells were stained with rabbit anti-NSP5a3a then with anti-rabbit Tritc secondary. Subsequently, MCF-12a cells were stained with mouse anti-B23 then with anti-rabbit Fitc secondary to correlate how NSP 5a3a localizes with respect to B23. Controls consisted of MCF-12A cells stained with anti-rabbit Tritc secondary only and anti-rabbit Fitc secondary only. Nuc (nucleus): nuc (nucleoli).

Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with rabbit polyclonal anti-NSP 5a3a (Novus) at 1/500 dilution in 1% BSA over night at 4°C.

Techniques: Immunostaining, Staining

Figure 4. Western blot analysis of Co-immunoprecipitations of MCF-7 and MCF-12A total cell lysates. (A) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (B) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (C) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (D) MCF-7 cell lysate im- munoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (E) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (F) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (G) MCF-12A cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (H) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (I) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (J) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (K) MCF-7 cell lysate immuno- precipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (L) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23.

Journal: Cell cycle (Georgetown, Tex.)

Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

doi: 10.4161/cc.9.6.10991

Figure Lengend Snippet: Figure 4. Western blot analysis of Co-immunoprecipitations of MCF-7 and MCF-12A total cell lysates. (A) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (B) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (C) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (D) MCF-7 cell lysate im- munoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (E) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (F) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (G) MCF-12A cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (H) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (I) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (J) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (K) MCF-7 cell lysate immuno- precipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (L) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23.

Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with rabbit polyclonal anti-NSP 5a3a (Novus) at 1/500 dilution in 1% BSA over night at 4°C.

Techniques: Western Blot, Immunoprecipitation

Figure 6. Western blot analysis of co-immunoprecipitations of MCF-7 cytoplasmic and nuclear fractions. (A) MCF-7 cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (B) MCF-7 cytoplasmic immunoprecipitation using anti-B23 and western blot analy- sis using anti-hnRNP-L. (C) MCF-7 cytoplasmic immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (D) MCF-7 cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (E) MCF-7 nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (F) MCF-7 nuclear immunoprecipitation using anti-B23 and western blot analysis using anti- hnRNP-L. (G) MCF-7 nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (H) MCF-7 nuclear immunoprecipita- tion using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (I) MCF-7 nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (J) MCF-7 nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (K) Cytoplas- mic marker alpha-tubulin. (L) Nuclear marker Lamin A/C.

Journal: Cell cycle (Georgetown, Tex.)

Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

doi: 10.4161/cc.9.6.10991

Figure Lengend Snippet: Figure 6. Western blot analysis of co-immunoprecipitations of MCF-7 cytoplasmic and nuclear fractions. (A) MCF-7 cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (B) MCF-7 cytoplasmic immunoprecipitation using anti-B23 and western blot analy- sis using anti-hnRNP-L. (C) MCF-7 cytoplasmic immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (D) MCF-7 cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (E) MCF-7 nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (F) MCF-7 nuclear immunoprecipitation using anti-B23 and western blot analysis using anti- hnRNP-L. (G) MCF-7 nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (H) MCF-7 nuclear immunoprecipita- tion using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (I) MCF-7 nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (J) MCF-7 nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (K) Cytoplas- mic marker alpha-tubulin. (L) Nuclear marker Lamin A/C.

Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with rabbit polyclonal anti-NSP 5a3a (Novus) at 1/500 dilution in 1% BSA over night at 4°C.

Techniques: Western Blot, Immunoprecipitation, Marker

Figure 5. Western blot analysis of co-immunoprecipitations of MCF-12A cytoplasmic and nuclear fractions. (A) MCF-12A cytoplasmic immunoprecipi- tation using anti-hnRNP-L and western blot analysis using anti-B23. (B) MCF-12A cytoplasmic immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (C) MCF-12A cytoplasmic immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (D) MCF-12A cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (E) MCF-12A cytoplasmic immunopre- cipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (F) MCF-12A cytoplasmic immunoprecipitation using anti-NSP 5a3a and west- ern blot analysis using anti-B23. (G) MCF-12A nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (H) MCF-12A nuclear immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (I) MCF-12A nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (J) MCF-12A nuclear immunoprecipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (K) Cytoplasmic marker alpha-tubulin. (L) Nuclear marker Lamin A/C.

Journal: Cell cycle (Georgetown, Tex.)

Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

doi: 10.4161/cc.9.6.10991

Figure Lengend Snippet: Figure 5. Western blot analysis of co-immunoprecipitations of MCF-12A cytoplasmic and nuclear fractions. (A) MCF-12A cytoplasmic immunoprecipi- tation using anti-hnRNP-L and western blot analysis using anti-B23. (B) MCF-12A cytoplasmic immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (C) MCF-12A cytoplasmic immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (D) MCF-12A cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (E) MCF-12A cytoplasmic immunopre- cipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (F) MCF-12A cytoplasmic immunoprecipitation using anti-NSP 5a3a and west- ern blot analysis using anti-B23. (G) MCF-12A nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (H) MCF-12A nuclear immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (I) MCF-12A nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (J) MCF-12A nuclear immunoprecipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (K) Cytoplasmic marker alpha-tubulin. (L) Nuclear marker Lamin A/C.

Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with rabbit polyclonal anti-NSP 5a3a (Novus) at 1/500 dilution in 1% BSA over night at 4°C.

Techniques: Western Blot, Immunoprecipitation, Marker