Journal: The FASEB Journal
Article Title: Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats
doi: 10.1096/fj.201802772R
Figure Lengend Snippet: Primer sequences and product size
Article Snippet: Detection steps used the following primary antibodies; 8-oxoguanine DNA glycosylase (OGG1; NB100-106, 1:1000; Novus Biologicals, Centennial, CO, USA), NTH1 (11154-1-AP, 1:1000; Proteintech, Rosemont, IL, USA), hypoxia-inducible factor 1α (HIF1α; Ab51608, 1:1000; Abcam, Cambridge, MA, USA), catalase (Ab1877-10, 1:10,000; Abcam), MnSOD (06–984; Upstate, Watford, United Kingdom), copper/zinc superoxide dismutase (CuZnSOD; 10269-1-AP, 1:1000; Proteintech), NADPH oxidase 1 (GP91 phox ; 19013-1-AP; Proteintech), neutrophil cytosolic factor 2 (P67 phox ; 15551-1-AP, 1:1000; Proteintech), xanthine oxidase (XO; SC-20991, 1:200; Santa Cruz Biotechnology, Dallas, TX, USA), tumor protein 53 (P53; MAB1355, 1:1000; R&D Systems, Minneapolis, MN, USA), cyclin-dependent kinase inhibitor 2A (P16 ink ; Ab189034, 1:1000; Abcam), KU70 (10723-1-AP, 1:1000; Proteintech), KU80 (NB100-508, 1:1000; Novus Biologicals).
Techniques: Sequencing