Journal: Cell Genomics
Article Title: NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors
doi: 10.1016/j.xgen.2024.100550
Figure Lengend Snippet:
Article Snippet: The primary antibodies used here were anti-NRDE2 (1:200; orb221834-CF405M, Biorbyt, USA), anti-γ-H2AX (1:500; 05–636, Millipore, USA), anti-RPA2 (1:200; 10412-1-AP, Proteintech, China), anti-RAD51 (1:150; ab88572, Abcam, USA), anti-53BP1 (1:250; NB100-304, Novus Biologicals, USA), anti-MDC1 (1:250; NB100-395, Novus Biologicals, USA), anti-NBS1 (1:200; 55025-1-AP, Proteintech, China), anti-MRE11 (1:200; 10744-1-AP, Proteintech, China) and anti-RAD50 (1:400; 29390-1-AP, Proteintech, China).
Techniques: Phospho-proteomics, Recombinant, Staining, SYBR Green Assay, Protease Inhibitor, Cell Fractionation, CCK-8 Assay, Sequencing, RNA Sequencing, Software