Journal: International Journal of Molecular Sciences

Article Title: Engineering a 3D In Vitro Model of Human Gingival Tissue Equivalent with Genipin/Cytochalasin D

doi: 10.3390/ijms23137401

Figure Lengend Snippet: Quantitative RT-PCR assay [( A ) = K14, ( B ) = K10, ( C ) = Involucrin (Inv.), ( D ) = collagen 1a1 and ( E ) = CD90] of the 3D GTEs generated with the TIGKs and the hGFBs-populated collagen gels with/without genipin/cytochalasin D direct crosslinking for 2-week air–liquid interface culture. Abbreviations: hGFBs: hTERT-immortalized gingival fibroblast; TIGKs: hTERT-immortalized gingival keratinocytes. Ctrl: control; G50: 50 µM genipin; C100: 100 nM cytochalasin D; G + C: G50 + C100. *: p < 0.05.

Article Snippet: After antigen retrieval by citrate buffer, the sections were blocked with 5% FBS, and then incubated overnight at 4 °C with corresponding primary antibodies against human K14 (1:400), K10 (1:2500, ab76318, Abcam, Cambridge, UK), involucrin (1:250, NB100-2727 SY5, Novus biological, Thermofisher Scientific), Ki-67 (1:50, P6834, Sigma-Aldrich), p53 (1:100, Sc-47698, Santa Cruz Biotechnology), vimentin (1:500, EPR3776, Abcam).

Techniques: Quantitative RT-PCR, Generated, Control

Journal: International Journal of Molecular Sciences

Article Title: Engineering a 3D In Vitro Model of Human Gingival Tissue Equivalent with Genipin/Cytochalasin D

doi: 10.3390/ijms23137401

Figure Lengend Snippet: Immunohistochemical staining (5-µm section) of HE, K14, K10, Inv., and vimentin in human 3D gingival tissue equivalents (GTEs) generated with TIGKs and hGFBs-populated collagen gel with/without genipin/cytochalasin D direct crosslinking for 2-week air–liquid interface culture. Abbreviations: hGFBs: hTERT-immortalized gingival fibroblasts; TIGKs: hTERT-immortalized gingival keratinocytes; G50: 50 µM genipin; C100: 100 nM cytochalasin D; G + C: G50 + C100; HE: hematoxylin & eosin; K14: keratin 14; K10: keratin 10; Inv.: involucrin.

Article Snippet: After antigen retrieval by citrate buffer, the sections were blocked with 5% FBS, and then incubated overnight at 4 °C with corresponding primary antibodies against human K14 (1:400), K10 (1:2500, ab76318, Abcam, Cambridge, UK), involucrin (1:250, NB100-2727 SY5, Novus biological, Thermofisher Scientific), Ki-67 (1:50, P6834, Sigma-Aldrich), p53 (1:100, Sc-47698, Santa Cruz Biotechnology), vimentin (1:500, EPR3776, Abcam).

Techniques: Immunohistochemical staining, Staining, Generated