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immunoblotting α cdc2  (Novus Biologicals)
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Novus Biologicals immunoblotting α cdc2
Depletion of Rad52 and Mad2 using the AID system results in loss of viability in diploids. A, b) Immunoblot and quantification of Mad2 degradation in Mad2-AID haploids and Mad2-AID homozygous diploids. Cells were treated with 100 nM 5′a-IAA and sampled at the indicated time points. <t>Cdc2</t> was used as the loading control. c) Control and Mad2-AID strains were spotted onto YES plates with and without 100 nM 5′a-IAA and/or 10 µg/mL thiabendazole (TBZ). Plates were incubated at 32°C for 3 days and then imaged. d) Immunoblot and quantification of Rad52 degradation in Rad52-AID homozygous diploids. Conditions were the same as the Mad2 assay. e) Control and Rad52-AID strains were spotted onto YES plates with and without 100 nM 5′a-IAA and/or 2 mM HU. Plates were incubated at 32°C for 3 days and then imaged.
Immunoblotting α Cdc2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunoblotting α cdc2 - by Bioz Stars, 2026-04
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Depletion of Rad52 and Mad2 using the AID system results in loss of viability in diploids. A, b) Immunoblot and quantification of Mad2 degradation in Mad2-AID haploids and Mad2-AID homozygous diploids. Cells were treated with 100 nM 5′a-IAA and sampled at the indicated time points. Cdc2 was used as the loading control. c) Control and Mad2-AID strains were spotted onto YES plates with and without 100 nM 5′a-IAA and/or 10 µg/mL thiabendazole (TBZ). Plates were incubated at 32°C for 3 days and then imaged. d) Immunoblot and quantification of Rad52 degradation in Rad52-AID homozygous diploids. Conditions were the same as the Mad2 assay. e) Control and Rad52-AID strains were spotted onto YES plates with and without 100 nM 5′a-IAA and/or 2 mM HU. Plates were incubated at 32°C for 3 days and then imaged.

Journal: Genetics

Article Title: Diploidy confers genomic instability in Schizosaccharomyces pombe

doi: 10.1093/genetics/iyaf078

Figure Lengend Snippet: Depletion of Rad52 and Mad2 using the AID system results in loss of viability in diploids. A, b) Immunoblot and quantification of Mad2 degradation in Mad2-AID haploids and Mad2-AID homozygous diploids. Cells were treated with 100 nM 5′a-IAA and sampled at the indicated time points. Cdc2 was used as the loading control. c) Control and Mad2-AID strains were spotted onto YES plates with and without 100 nM 5′a-IAA and/or 10 µg/mL thiabendazole (TBZ). Plates were incubated at 32°C for 3 days and then imaged. d) Immunoblot and quantification of Rad52 degradation in Rad52-AID homozygous diploids. Conditions were the same as the Mad2 assay. e) Control and Rad52-AID strains were spotted onto YES plates with and without 100 nM 5′a-IAA and/or 2 mM HU. Plates were incubated at 32°C for 3 days and then imaged.

Article Snippet: The following antibodies and dilutions were used for immunoblotting: α- cdc2 (Novus Biologicals NB100-2716; 1:2,000), α-V5 (Abcam ab27671; 1:1,000), α-myc (Abcam ab9106 and Novus Biologicals NBP2-52636; 1:1,000), α-Alpha Tubulin (Millipore Sigma T5168; 1:1,000), α-Rabbit Alexa Fluor 488 (Thermo Scientific A32790; 1:1,000), and α-Mouse Alexa Fluor 488 (Thermo Scientific A28175; 1:1,000).

Techniques: Western Blot, Control, Incubation