Journal: eLife

Article Title: ZCWPW1 is recruited to recombination hotspots by PRDM9 and is essential for meiotic double strand break repair

doi: 10.7554/eLife.53392

Figure Lengend Snippet:

Article Snippet: ChIP was performed in 10 mM Tris-HCl pH8, 1 mM EDTA, 0.1% Sodium Deoxycholate, 1% Triton X-100, 500 mM NaCl using 5 μg of mouse anti-DMC1 2H12/4 (Novus NB100-2617, RRID: AB_2245859 ) pre-bound to 50 μl of Dynabeads M-280 sheep anti-mouse IgG (Thermo Fisher Scientific 11202D, RRID: AB_772210 ).

Techniques: Knock-Out, CRISPR, Transfection, Construct, Clone Assay, Plasmid Preparation, Sequencing, Variant Assay, Generated, Cloning, Recombinant, Bicinchoninic Acid Protein Assay, Western Blot, SYBR Green Assay, RNA Extraction, Expressing, Purification, Protease Inhibitor, Software