Journal: Frontiers in Physiology
Article Title: The effects of 4-Phenylbutyric acid on ER stress during mouse tooth development
doi: 10.3389/fphys.2022.1079355
Figure Lengend Snippet: Histology and localization patterns of ER stress markers in mouse developing teeth. HE staining showing embryonic tooth germs at cap (A) and bell stage (B) of tooth development. Localizations of GRP78 and HRD1 at E14 and E16 tooth germs and counterstained with hematoxylin (C–F) . Dotted lines demarcate epithelial boundary (A–F) . Arrows indicate immunostaining positive cells. EK, enamel knot; IEE, inner enamel epithelium; OEE, outer enamel epithelium; DP, dental papilla; SR, stellate reticulum. Scale bars: 200 μm (A,C and E) , 50 μm (B,D and F) .
Article Snippet: Primary antibodies used in this study are Nestin (Abcam, Cambridge, United Kingdom, 1:400; cat. no. ab11306), Amelogenin (AMELX; Abcam, Cambridge, United Kingdom, 1:500; cat. no. ab153915), Glucose regulatory protein 78 (GRP78; Abcam, Cambridge, United Kingdom, 1:400; cat. no. ab21685) and HRD1 (Novus Biologicals, Colorado, United States, 1:200; cat. no. NB100-2526) and Ki67 (Thermo Scientific, Waltham, MA, United States, 1:400; cat. no. RM-9106-s).
Techniques: Staining, Immunostaining