Journal: American Journal of Physiology - Cell Physiology

Article Title: SEMA3B inhibits TGFβ-induced extracellular matrix protein production and its reduced levels are associated with a decline in lung function in IPF

doi: 10.1152/ajpcell.00681.2023

Figure Lengend Snippet: Reduced semaphorin class 3B (SEMA3B) and neuropilin 1 (NRP1) levels associate with lung function loss in idiopathic pulmonary fibrosis (IPF). A : SEMA3B transcript levels were lower in patients with IPF ( n = 160) compared to healthy subjects ( n = 108) based on the RNA microarray dataset GSE47460. **** P < 0.0001. B and C : GSE47460 data show a positive correlation between SEMA3B transcript levels and both percent forced vital capacity (FVC) and diffusing capacity of the lungs for carbon monoxide (DL CO ) in both IPF (red) and healthy subjects (blue). The blue line represents the linear correlation. D : RNA-sequencing data from GSE150910 confirms decreased SEMA3B transcript levels in IPF lungs compared to healthy controls (CTRL; n = 40–60/group). **** P < 0.0001. E and F : similar to GSE47460, GSE150910 data show a positive correlation between SEMA3B transcript levels and percent FVC or DL CO in IPF (red) and healthy subjects (blue). G : NRP1 transcript levels were also reduced in IPF lungs compared to healthy lungs according to the RNA-sequencing data from GSE150910. **** P < 0.0001. H and I : a positive correlation between NRP1 transcript levels and both percent FVC and DL CO is observed in IPF (red) and healthy subjects (blue) from the GSE150910 dataset. The correlation coefficient ( r ) and P value are displayed within the scatter plots.

Article Snippet: Formalin-fixed and paraffin-embedded human lung tissue sections of IPF ( n = 6) and healthy ( n = 5) controls were probed with antibodies against SEMA3B (Novus Biologicals) or NRP1 (Proteintech) for immunohistochemistry staining as previously described ( , ).

Techniques: Microarray, RNA Sequencing

Journal: American Journal of Physiology - Cell Physiology

Article Title: SEMA3B inhibits TGFβ-induced extracellular matrix protein production and its reduced levels are associated with a decline in lung function in IPF

doi: 10.1152/ajpcell.00681.2023

Figure Lengend Snippet: Downregulation of semaphorin class 3B (SEMA3B) and neuropilin 1 (NRP1) in idiopathic pulmonary fibrosis (IPF) lungs. A : violin plot showing the expression levels of SEMA3B and NRP1 across various cell types in non-IPF and IPF lungs. Data are from a publicly available dataset (GSE136831) for non-IPF and IPF lung scRNA-seq. B and C : representative immunohistochemical images of distal lung biopsies from normal and IPF lungs stained with antibodies against SEMA3B ( B ) and NRP1 ( C ). Scale bar = 50 μm. Arrows highlight spindle-shaped mesenchymal cells positive for SEMA3B or NRP1 ( n = 5 per group). D and E : transcript levels of SEMA3B and NRP1 were quantified in fibroblasts isolated from the lungs of IPF patients and healthy controls using RT-PCR. Student’s t test was performed with n = 10–14 per group. *** P < 0.001, ** P < 0.01.

Article Snippet: Formalin-fixed and paraffin-embedded human lung tissue sections of IPF ( n = 6) and healthy ( n = 5) controls were probed with antibodies against SEMA3B (Novus Biologicals) or NRP1 (Proteintech) for immunohistochemistry staining as previously described ( , ).

Techniques: Expressing, Immunohistochemical staining, Staining, Isolation, Reverse Transcription Polymerase Chain Reaction

Journal: American Journal of Physiology - Cell Physiology

Article Title: SEMA3B inhibits TGFβ-induced extracellular matrix protein production and its reduced levels are associated with a decline in lung function in IPF

doi: 10.1152/ajpcell.00681.2023

Figure Lengend Snippet: Semaphorin class 3B (SEMA3B) inhibits TGFβ1-driven extracellular matrix (ECM) gene expression in fibroblasts. A : upregulation of the transcripts of ECM genes [collagen 1A1 (COL1A1), elastin (ELN), and α-smooth muscle actin (αSMA)] in normal lung fibroblasts treated with media, transforming growth factor-β (TGFβ), SEMA3B, or both TGFβ and SEMA3B for 16 h. B : upregulation of the transcripts of ECM genes (COL1A1, ELN, and αSMA) in IPF fibroblasts treated with media, TGFβ, SEMA3B, or both TGFβ and SEMA3B for 16 h. Data are shown as the means ± SE. Student’s t test was performed with n = 4/group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Formalin-fixed and paraffin-embedded human lung tissue sections of IPF ( n = 6) and healthy ( n = 5) controls were probed with antibodies against SEMA3B (Novus Biologicals) or NRP1 (Proteintech) for immunohistochemistry staining as previously described ( , ).

Techniques: Gene Expression

Journal: American Journal of Physiology - Cell Physiology

Article Title: SEMA3B inhibits TGFβ-induced extracellular matrix protein production and its reduced levels are associated with a decline in lung function in IPF

doi: 10.1152/ajpcell.00681.2023

Figure Lengend Snippet: Semaphorin class 3B (SEMA3B) inhibits the transforming growth factor-β1 (TGFβ1)-driven extracellular matrix (ECM) protein production in Idiopathic pulmonary fibrosis (IPF) lung fibroblasts. A : IPF lung fibroblasts were treated with media, SEMA3B (400 ng/mL), and/or TGFβ1 (20 ng/mL) for 72 hours, and cell lysates were immunoblotted with antibodies against collagen 1 (COL1), fibronectin 1 (FN1), α-smooth muscle actin (αSMA), and GAPDH. B : IPF lung fibroblasts were treated with media, SEMA3B (400 ng/mL), and/or TGFβ1 (20 ng/mL) for 72 h, and cell lysates were immunoblotted with antibodies against elastin (ELN) and GAPDH. C : densitometric quantification of COL1, FN1, αSMA, and ELN protein levels normalized to GAPDH. Data are shown as the means ± SE. A one-way ANOVA test was performed with n = 3/group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Formalin-fixed and paraffin-embedded human lung tissue sections of IPF ( n = 6) and healthy ( n = 5) controls were probed with antibodies against SEMA3B (Novus Biologicals) or NRP1 (Proteintech) for immunohistochemistry staining as previously described ( , ).

Techniques:

Journal: Medical Science Monitor

Article Title: Expression of Semaphorin 3B (SEMA3B) in Various Grades of Endometrial Cancer

doi: 10.12659/msm.916762

Figure Lengend Snippet: Figure 1. Immunohistochemical localization of SEMA3B in different grades of endometrial cancer. C – control; G – grade of endometrial cancer. 200× magnification.

Article Snippet: The analysis of SEMA3B level changes was performed based on the immunohistochemical reaction with rabbit polyclonal anti-SEMA3B antibody (Novus Biological).

Techniques: Immunohistochemical staining, Control