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FIG. 7. ATM and CHK2 target proteins activated by irofulven. A and B, human ovarian cancer cell lines A2780, CAOV3, SKOV3, and OVCAR3 were treated with 1 IC50 concentration of irofulven (A); and human normal fibroblast GM00637 and AT fibroblast GM05849 were treated with 8 M of irofulven (B) for 1 h followed by additional 24 h of incubation. Western blot analyses were performed with antibodies against phosphorylated NBS1 on Ser343, NBS1, phosphorylated SMC1 on <t>Ser957,</t> SMC1, and phosphorylated p53 on Ser15. The nonspecific band was indicated as n.s. C and D, A2780 and CAOV3 cells (C); and human colon cancer cell line HCT116 and its CHK2 knockout subline (D) were treated with 1 IC50 concentration of irofulven for 1 h followed by additional 24 h of incubation, Western blots were performed with antibodies against phosphorylated p53 on Ser20, p53, and actin.
Ser957, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ser957/product/Novus Biologicals
Average 90 stars, based on 1 article reviews
ser957 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

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FIG. 7. ATM and CHK2 target proteins activated by irofulven. A and B, human ovarian cancer cell lines A2780, CAOV3, SKOV3, and OVCAR3 were treated with 1 IC50 concentration of irofulven (A); and human normal fibroblast GM00637 and AT fibroblast GM05849 were treated with 8 M of irofulven (B) for 1 h followed by additional 24 h of incubation. Western blot analyses were performed with antibodies against phosphorylated NBS1 on Ser343, NBS1, phosphorylated SMC1 on Ser957, SMC1, and phosphorylated p53 on Ser15. The nonspecific band was indicated as n.s. C and D, A2780 and CAOV3 cells (C); and human colon cancer cell line HCT116 and its CHK2 knockout subline (D) were treated with 1 IC50 concentration of irofulven for 1 h followed by additional 24 h of incubation, Western blots were performed with antibodies against phosphorylated p53 on Ser20, p53, and actin.

Journal: Journal of Biological Chemistry

Article Title: ATM-dependent CHK2 Activation Induced by Anticancer Agent, Irofulven

doi: 10.1074/jbc.m400015200

Figure Lengend Snippet: FIG. 7. ATM and CHK2 target proteins activated by irofulven. A and B, human ovarian cancer cell lines A2780, CAOV3, SKOV3, and OVCAR3 were treated with 1 IC50 concentration of irofulven (A); and human normal fibroblast GM00637 and AT fibroblast GM05849 were treated with 8 M of irofulven (B) for 1 h followed by additional 24 h of incubation. Western blot analyses were performed with antibodies against phosphorylated NBS1 on Ser343, NBS1, phosphorylated SMC1 on Ser957, SMC1, and phosphorylated p53 on Ser15. The nonspecific band was indicated as n.s. C and D, A2780 and CAOV3 cells (C); and human colon cancer cell line HCT116 and its CHK2 knockout subline (D) were treated with 1 IC50 concentration of irofulven for 1 h followed by additional 24 h of incubation, Western blots were performed with antibodies against phosphorylated p53 on Ser20, p53, and actin.

Article Snippet: Polyclonal antibodies against phosphorylated NBS1 on Ser343 and phosphorylated SMC1 on Ser957 were purchased from Novus Biologicals (Littleton, CO).

Techniques: Concentration Assay, Incubation, Western Blot, Knock-Out