Journal: Cancers

Article Title: The Expression of CD74-Regulated Inflammatory Markers in Stage IV Melanoma: Risk of CNS Metastasis and Patient Survival

doi: 10.3390/cancers12123754

Figure Lengend Snippet: Overall Survival by CD74 Number. Clinical outcomes by CD74 in tumor cells in stage IV melanoma patients in datasets. Kaplan-Meier analysis for OS for the expression by percentage of staining. n = negative (<5% cells are positive), p = positive (=/>5% cells are positive).

Article Snippet: We used anti-iNOS monoclonal antibody (Creative Biolabs, Shirley, NY, USA), a rabbit anti-NT polyclonal antibody (Millipore, Temecula, CA, USA), a rabbit anti-Cox-2 polyclonal antibody (Cell Signaling, Danvers, MA, USA), a mouse anti-CD74 monoclonal antibody (PIN.1, Novus Biologicals, Littleton, CO, USA), a goat anti-MIF polyclonal antibody and mPGES-1 (Novus Biologicals).

Techniques: Expressing, Staining

Journal: Cancers

Article Title: The Expression of CD74-Regulated Inflammatory Markers in Stage IV Melanoma: Risk of CNS Metastasis and Patient Survival

doi: 10.3390/cancers12123754

Figure Lengend Snippet: Overall survival for combined markers. Clinical outcomes by combined markers of CD74 and MIF in tumor cells in stage IV melanoma patients in datasets. Kaplan-Meier analysis for OS for their expression by intensity of staining. n = negative (no staining), p = positive (light to intense level of staining).

Article Snippet: We used anti-iNOS monoclonal antibody (Creative Biolabs, Shirley, NY, USA), a rabbit anti-NT polyclonal antibody (Millipore, Temecula, CA, USA), a rabbit anti-Cox-2 polyclonal antibody (Cell Signaling, Danvers, MA, USA), a mouse anti-CD74 monoclonal antibody (PIN.1, Novus Biologicals, Littleton, CO, USA), a goat anti-MIF polyclonal antibody and mPGES-1 (Novus Biologicals).

Techniques: Expressing, Staining

Journal: Cancers

Article Title: The Expression of CD74-Regulated Inflammatory Markers in Stage IV Melanoma: Risk of CNS Metastasis and Patient Survival

doi: 10.3390/cancers12123754

Figure Lengend Snippet: A Volcano Plot of the Genes in Association with CD74 in Stage IV Melanoma. The genes on the left side are lower in the “CD74 low” group compared to the “CD74 high” samples, while the genes on the right side have higher expression in the “CD74 low” samples.

Article Snippet: We used anti-iNOS monoclonal antibody (Creative Biolabs, Shirley, NY, USA), a rabbit anti-NT polyclonal antibody (Millipore, Temecula, CA, USA), a rabbit anti-Cox-2 polyclonal antibody (Cell Signaling, Danvers, MA, USA), a mouse anti-CD74 monoclonal antibody (PIN.1, Novus Biologicals, Littleton, CO, USA), a goat anti-MIF polyclonal antibody and mPGES-1 (Novus Biologicals).

Techniques: Expressing

Journal: Cancers

Article Title: The Expression of CD74-Regulated Inflammatory Markers in Stage IV Melanoma: Risk of CNS Metastasis and Patient Survival

doi: 10.3390/cancers12123754

Figure Lengend Snippet: Tumor CD74 and NT expression characteristics by immunohistochemical analyses. Stage IV melanoma tissues from patients were immunostained for CD74 and NT. As published previously (10), both intensity and percentage of positive cells were quantified and protein expression associating with overall survival (OS) in this retrospective tissue microarray. Representative tumor CD74 positive ( a ) and CD74 negative ( b ), NT positive ( c ) and NT negative ( d ) specimens are shown (400× magnification). Both positive samples are scored as 3 for both intensity (intense staining) and number (>75% of cells stained) and negative samples are 0 for both intensity (no staining) and number (<5% of cells stained).

Article Snippet: We used anti-iNOS monoclonal antibody (Creative Biolabs, Shirley, NY, USA), a rabbit anti-NT polyclonal antibody (Millipore, Temecula, CA, USA), a rabbit anti-Cox-2 polyclonal antibody (Cell Signaling, Danvers, MA, USA), a mouse anti-CD74 monoclonal antibody (PIN.1, Novus Biologicals, Littleton, CO, USA), a goat anti-MIF polyclonal antibody and mPGES-1 (Novus Biologicals).

Techniques: Expressing, Immunohistochemical staining, Microarray, Staining