smn antibody (2b1)  (Bio-Techne corporation)

Journal: International Journal of Molecular Sciences

Article Title: Physiological Features of the Neural Stem Cells Obtained from an Animal Model of Spinal Muscular Atrophy and Their Response to Antioxidant Curcumin

doi: 10.3390/ijms25158364

Figure Lengend Snippet: Evaluation of SMA protein levels. Western blot analysis of the SMN protein extracted from WT-ctr and SMA-ctr. Three WT-ctr protein samples and three or four SMA-ctr samples were separated through electrophoresis and transferred to a PVDF membrane. Total protein per lane was measured (as a normalization factor), and SMN protein levels were detected using NB100-1936 Novus Biologicals antibody. White: WT-ctr; green: SMA-ctr. **** p < 0.0001. Statistical analysis was performed using the one-tailed unpaired t -test.

Article Snippet: Using a Trans-blot ® Turbo TM Transfer System (Bio-Rad, CA, USA), proteins were transferred to nitrocellulose membranes (using a Trans-blot Turbo RTA transfer kit PVDF 1704272 (Bio-Rad, CA, USA) set to 1.3A-25V for 10 min) and blocked with 5% dry milk (Merck, Darmstadt, Germany) in Tween-Tris Buffer Saline Solution (0.05% Tween 20) at room temperature for 3 h. The membranes were then incubated with antibodies against SMN (NB100-1936 (Novus Biologicals, Littleton, CO, USA)) or other epitopes overnight at 4 °C.

Techniques: Western Blot, Electrophoresis, Membrane, One-tailed Test

Journal: International Journal of Molecular Sciences

Article Title: Physiological Features of the Neural Stem Cells Obtained from an Animal Model of Spinal Muscular Atrophy and Their Response to Antioxidant Curcumin

doi: 10.3390/ijms25158364

Figure Lengend Snippet: Evaluation of SMA protein levels after Cur treatment. Western blot analysis of the SMN protein extracted from WT-Cur and SMA-Cur. Three WT-Cur protein samples and three or four SMA-Cur samples were separated through electrophoresis and transferred to a PVDF membrane. Total protein per lane was measured (as a normalization factor), and SMN protein levels were detected using NB100-1936 Novus Biologicals antibody. Green: SMA-ctr; green pattern: SMA-Cur. $ p = 0.0475. Statistical analysis was performed using the one-tailed paired t -test.

Article Snippet: Using a Trans-blot ® Turbo TM Transfer System (Bio-Rad, CA, USA), proteins were transferred to nitrocellulose membranes (using a Trans-blot Turbo RTA transfer kit PVDF 1704272 (Bio-Rad, CA, USA) set to 1.3A-25V for 10 min) and blocked with 5% dry milk (Merck, Darmstadt, Germany) in Tween-Tris Buffer Saline Solution (0.05% Tween 20) at room temperature for 3 h. The membranes were then incubated with antibodies against SMN (NB100-1936 (Novus Biologicals, Littleton, CO, USA)) or other epitopes overnight at 4 °C.

Techniques: Western Blot, Electrophoresis, Membrane, One-tailed Test