dec1 antibody - bsa free  (Bio-Techne corporation)

Journal: bioRxiv

Article Title: Comprehensive Multimodal Profiling of Atherosclerosis Reveals Bhlhe40 as a Potential Regulator of Vascular Smooth Muscle Cell Phenotypic Modulation

doi: 10.1101/2025.05.20.655228

Figure Lengend Snippet: (A-B) Heatmaps from re-analysis of ZsGreen1⁺ cells reveal distinct gene expression profiles in VSMC-derived foam cells compared to non-foamy counterparts at 16 (A) and 26 weeks (B) of Western diet feeding. (C-D) Volcano plots showing the dysregulation of genes involved in VSMC phenotypic modulation, lipid metabolism, and proliferation at 16 (C) and 26 weeks (D) of WD feeding. (E) Ingenuity Pathway Analysis of biological functions associated with VSMC-derived (ZsGreen1⁺) foam cells. (F-G) Top transcription factors inferred to be activated in VSMC-derived foam cells at 16 (F) and 26 weeks (G) of Western diet feeding. (H) Comparison of activated transcription factors at 16- and 26-weeks WD feeding. (I-J) Box plots showing expression of Bhlhe40 in VSMC Foamy (ZsGreen1 + LipidTOX + ) cells relative to VSMC non-Foamy (ZsGreen1 + LipidTOX - ) cells at 16 (I) and 26 weeks (J) WD feeding.

Article Snippet: Efficient knockdown of Bhlhe40 was validated by qPCR and Western blotting of protein extracts from transfected cells with rabbit anti-Dec1 (Bhlhe40) antibody (Novus Biologicals, NB100–1800, Lot C1).

Techniques: Gene Expression, Derivative Assay, Western Blot, Comparison, Expressing

Journal: bioRxiv

Article Title: Comprehensive Multimodal Profiling of Atherosclerosis Reveals Bhlhe40 as a Potential Regulator of Vascular Smooth Muscle Cell Phenotypic Modulation

doi: 10.1101/2025.05.20.655228

Figure Lengend Snippet: (A) Dot plot showing expression of canonical lipid metabolism genes across VSMC-lineage cells from the CITE-seq analysis , highlighting upregulation in modulated, proliferative, and macrophage-like subpopulations. (B) Pseudotime analysis illustrating the developmental trajectory of VSMCs transitioning from a contractile to modulated states during atherosclerosis progression. (C) Upstream regulatory analysis identifying transcriptional factors upregulated in modulated and proliferative VSMC subpopulations. (D) UMAP showing expression of transcription factors Klf6, Cebpb, and Bhlhe40—identified in both single-cell and bulk RNA-seq datasets— highlighting the selective enrichment of Bhlhe40 in modulated and proliferative VSMC subpopulations. (E) Dual RNAscope and immunofluorescence stain of lesions at 16 and 26 weeks WD feeding. Nuclei are stained with DAPI. Scale bar = 100µm.

Article Snippet: Efficient knockdown of Bhlhe40 was validated by qPCR and Western blotting of protein extracts from transfected cells with rabbit anti-Dec1 (Bhlhe40) antibody (Novus Biologicals, NB100–1800, Lot C1).

Techniques: Expressing, RNA Sequencing, RNAscope, Immunofluorescence, Staining

Journal: bioRxiv

Article Title: Comprehensive Multimodal Profiling of Atherosclerosis Reveals Bhlhe40 as a Potential Regulator of Vascular Smooth Muscle Cell Phenotypic Modulation

doi: 10.1101/2025.05.20.655228

Figure Lengend Snippet: Cultured primary VSMCs from C57BL/6J mice were treated with TNFα and MBD-Cholesterol (MBD-Chol) following siRNA-mediated Bhlhe40 knockdown. (A and B) Knockdown of Bhlhe40 led to altered expression of genes (A) and proteins (B) associated with VSMC modulation and foam cell phenotype. Statistics were analyzed by ANOVA with multiple comparison. Data represented as mean +/- standard error mean. P-value <0.05.

Article Snippet: Efficient knockdown of Bhlhe40 was validated by qPCR and Western blotting of protein extracts from transfected cells with rabbit anti-Dec1 (Bhlhe40) antibody (Novus Biologicals, NB100–1800, Lot C1).

Techniques: Cell Culture, Knockdown, Expressing, Comparison

Journal: bioRxiv

Article Title: Comprehensive Multimodal Profiling of Atherosclerosis Reveals Bhlhe40 as a Potential Regulator of Vascular Smooth Muscle Cell Phenotypic Modulation

doi: 10.1101/2025.05.20.655228

Figure Lengend Snippet: (A and B) UMAP visualization of re- analyzed published CITE-seq dataset of human carotid atherosclerosis of VSMCs/fibroblasts (A) and macrophages (B). (C) Proportion of macrophage subtypes in human lesions. (D-E) Dot plots showing the expression of canonical marker genes (D) and proteins (E) across VSMCs and Fibroblasts, and Macrophages. (F) UMAP showing the expression of BHLHE40 in modulated VSMC subpopulations. (G) Dual RNAscope and immunofluorescence staining of human coronary arteries showing colocalization of ACTA2 and BHLHE40, indicative of BHLHE40 expression in VSMCs within human lesions. Nuclei are stained with DAPI. Scale bar = 100µm. (H) Immunohistochemical staining identifies BHLHE40 expression in stable and unstable human carotid atherosclerotic plaques.

Article Snippet: Efficient knockdown of Bhlhe40 was validated by qPCR and Western blotting of protein extracts from transfected cells with rabbit anti-Dec1 (Bhlhe40) antibody (Novus Biologicals, NB100–1800, Lot C1).

Techniques: Expressing, Marker, RNAscope, Immunofluorescence, Staining, Immunohistochemical staining