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sec16a antibody  (Bio-Techne corporation)


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    Bio-Techne corporation sec16a antibody
    Sec16a Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sec16a antibody/product/Bio-Techne corporation
    Average 90 stars, based on 11 article reviews
    sec16a antibody - by Bioz Stars, 2026-04
    90/100 stars

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    (A) NRK cells transfected with the indicated siRNA were labeled with anti-ALG-2, anti-sec31A, or <t>anti-sec16A</t> antibodies, a cy3-conjugated secondary antibody, and dapi. Maximum intensity projections from deconvolved widefield z-stacks of the ERES proteins are displayed in grayscale merged with the corresponding dapi panel. (B) Quantitation of 4–5 fields of ~10 random cells per field, for each condition. A consistent intensity threshold was used to automatically capture and count bright cytoplasmic objects. A T-test between the siControl and siPeflin group was performed for each labeled protein: ALG-2, N = 85, p = 0.0003; Sec31A, N = 79, p = 0.0001; Sec16A, N = 100, p = 0.866.
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    (A) NRK cells transfected with the indicated siRNA were labeled with anti-ALG-2, anti-sec31A, or <t>anti-sec16A</t> antibodies, a cy3-conjugated secondary antibody, and dapi. Maximum intensity projections from deconvolved widefield z-stacks of the ERES proteins are displayed in grayscale merged with the corresponding dapi panel. (B) Quantitation of 4–5 fields of ~10 random cells per field, for each condition. A consistent intensity threshold was used to automatically capture and count bright cytoplasmic objects. A T-test between the siControl and siPeflin group was performed for each labeled protein: ALG-2, N = 85, p = 0.0003; Sec31A, N = 79, p = 0.0001; Sec16A, N = 100, p = 0.866.
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    (A) NRK cells transfected with the indicated siRNA were labeled with anti-ALG-2, anti-sec31A, or anti-sec16A antibodies, a cy3-conjugated secondary antibody, and dapi. Maximum intensity projections from deconvolved widefield z-stacks of the ERES proteins are displayed in grayscale merged with the corresponding dapi panel. (B) Quantitation of 4–5 fields of ~10 random cells per field, for each condition. A consistent intensity threshold was used to automatically capture and count bright cytoplasmic objects. A T-test between the siControl and siPeflin group was performed for each labeled protein: ALG-2, N = 85, p = 0.0003; Sec31A, N = 79, p = 0.0001; Sec16A, N = 100, p = 0.866.

    Journal: PLoS ONE

    Article Title: Penta-EF-Hand Protein Peflin Is a Negative Regulator of ER-To-Golgi Transport

    doi: 10.1371/journal.pone.0157227

    Figure Lengend Snippet: (A) NRK cells transfected with the indicated siRNA were labeled with anti-ALG-2, anti-sec31A, or anti-sec16A antibodies, a cy3-conjugated secondary antibody, and dapi. Maximum intensity projections from deconvolved widefield z-stacks of the ERES proteins are displayed in grayscale merged with the corresponding dapi panel. (B) Quantitation of 4–5 fields of ~10 random cells per field, for each condition. A consistent intensity threshold was used to automatically capture and count bright cytoplasmic objects. A T-test between the siControl and siPeflin group was performed for each labeled protein: ALG-2, N = 85, p = 0.0003; Sec31A, N = 79, p = 0.0001; Sec16A, N = 100, p = 0.866.

    Article Snippet: Rabbit anti-sec16A (product NB100-1799) was from Novus Biologicals.

    Techniques: Transfection, Labeling, Quantitation Assay