hif-1 alpha antibody (h1alpha67)  (Bio-Techne corporation)

Journal: International Journal of Oral Science

Article Title: Single-cell multi-omics sequencing reveals cell-specific transcriptomic and chromatin accessibility profiles in gut microbiome metabolite butyrate-produced pain modulation

doi: 10.1038/s41368-026-00432-9

Figure Lengend Snippet: Mapping transcriptional regulation of Matk in neuropeptide neurons. a Chromatin accessibility signals indicating transcriptional regulation landscapes across three groups, highlighting different gene regions of Matk with precise locus positions. b A schematic diagram illustrating binding sites of five TFs within the regulatory loci for the gene Matk . c Interaction network for Matk and its TFs. Two repressors (blue; Nfib, Arnt) were consistent with reduced Matk accessibility and expression after CFA injection, while three activators (red; Hif1a, Bhlhe40, Smad5) opposed the repression and promoted partial recovery with TB treatment. d A cross-group analysis correlates gene expression patterns (snRNA-seq) with chromatin enrichment profiles (snATAC-seq). TSS, transcription start site. CON, saline + vehicle; CFA, CFA +vehicle; TB, CFA + tributyrin

Article Snippet: For validating TF-produced chromatin accessibility regulation of target genes, we performed ChIP with antibodies against predicted TFs: Thap11 (Santa Cruz, sc-517366), Hinfp (Santa Cruz, sc-373855), Hif1a (Novus, NB100-105), or Yy1 (Santa Cruz, sc-7341), and binding regions of Nop14 , Matk , Idh3b , Ndst2 , and Tomm6 were analyzed by qPCR with the respective primers (Supplementary Table ).

Techniques: Binding Assay, Expressing, Injection, Gene Expression, Saline

Journal: International Journal of Oral Science

Article Title: Single-cell multi-omics sequencing reveals cell-specific transcriptomic and chromatin accessibility profiles in gut microbiome metabolite butyrate-produced pain modulation

doi: 10.1038/s41368-026-00432-9

Figure Lengend Snippet: Mapping transcriptional regulation of Tomm6 in OPCs. a Chromatin accessibility signals indicating transcriptional regulation landscapes across three groups, highlighting different gene regions of Tomm6 in OPCs with precise locus positions. b A schematic diagram illustrating binding sites of four TFs within the regulatory loci for the gene Tomm6 . c A dynamic interaction network of Tomm6 and its TFs. Two activators (red; Srebf2, Hif1a) promoted Tomm6 accessibility and expression following CFA injection, while two repressors (blue; Bhlhe41, Cebpd) were involved in normalization of Tomm6 accessibility and expression after TB treatment. TSS, transcription start site. CON, saline + vehicle; CFA, CFA +vehicle; TB, CFA + tributyrin

Article Snippet: For validating TF-produced chromatin accessibility regulation of target genes, we performed ChIP with antibodies against predicted TFs: Thap11 (Santa Cruz, sc-517366), Hinfp (Santa Cruz, sc-373855), Hif1a (Novus, NB100-105), or Yy1 (Santa Cruz, sc-7341), and binding regions of Nop14 , Matk , Idh3b , Ndst2 , and Tomm6 were analyzed by qPCR with the respective primers (Supplementary Table ).

Techniques: Binding Assay, Expressing, Injection, Saline

Journal: Cancer Cell International

Article Title: PAARH suppresses Kupffer cell phagocytosis to promote tumor liver metastasis by upregulating CD47

doi: 10.1186/s12935-026-04251-0

Figure Lengend Snippet: PAARH recruits HIF-1α to CD47 promoters and activates CD47 transcription. A CUT&RUN analysis of HIF-1α binding to CD47 promoter in SNU-398 cells with PAARH overexpression or control cultured under hypoxia. B CUT&RUN analysis of HIF-1α binding to CD47 promoter in HCCLM3 cells with PAARH knockdown or control cultured under hypoxia. C CUT&RUN analysis of HIF-1α binding to CD47 promoter in CHL-1 cells with PAARH overexpression or control cultured under hypoxia. D CUT&RUN analysis of HIF-1α binding to CD47 promoter in SW620 cells with PAARH knockdown or control cultured under hypoxia. E Luciferase activity in SNU-398 cells with PAARH overexpression or control cultured under hypoxia after transfection of luciferase reporters containing CD47 promoter. F Luciferase activity in HCCLM3 cells with PAARH knockdown or control cultured under hypoxia after transfection of luciferase reporters containing CD47 promoter. G Luciferase activity in CHL-1 cells with PAARH overexpression or control cultured under hypoxia after transfection of luciferase reporters containing CD47 promoter. H Luciferase activity in SW620 cells with PAARH knockdown or control cultured under hypoxia after transfection of luciferase reporters containing CD47 promoter. I Luciferase activity in SNU-398 cells with PAARH overexpression or control cultured under hypoxia after transfection of HIF-1α specific siRNAs and luciferase reporters containing CD47 promoter. J Luciferase activity in CHL-1 cells with PAARH overexpression or control cultured under hypoxia after transfection of HIF-1α specific siRNAs and luciferase reporters containing CD47 promoter. For E-J, results are shown as the relative ratio of firefly luciferase activity to renilla luciferase activity. K qPCR analysis of CD47 mRNA expression in SNU-398 cells with PAARH overexpression or control cultured under hypoxia after transfection of HIF-1α specific siRNAs. L Flow cytometry quantification of CD47 protein expression in SNU-398 cells with PAARH overexpression or control cultured under hypoxia after transfection of HIF-1α specific siRNAs. M qPCR analysis of CD47 mRNA expression in CHL-1 cells with PAARH overexpression or control cultured under hypoxia after transfection of HIF-1α specific siRNAs. N Flow cytometry quantification of CD47 protein expression in CHL-1 cells with PAARH overexpression or control cultured under hypoxia after transfection of HIF-1α specific siRNAs. Data are shown as mean ± SD of n = 3 biological replicates. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant, by Student’s t -test (A, C, E, G) or one-way ANOVA with Dunnett’s multiple comparisons test (B, D, F, H-N)

Article Snippet: The binding of HIF-1α to CD47 promoter were evaluated by CUT&RUN assays using the CUT&RUN Assay Kit (Cell Signaling Technology, Danvers, MA, USA) and HIF-1α antibody (Cat. No. NB100-105; Novus, Saint Louis, MO, USA).

Techniques: Binding Assay, Over Expression, Control, Cell Culture, Knockdown, Luciferase, Activity Assay, Transfection, Expressing, Flow Cytometry