nanog  (WuXi AppTec)

 
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  • 91
    Name:
    NANOG Antibody
    Description:
    Mouse Monoclonal Antibody Mab IgG1 k
    Catalog Number:
    am1486b
    Price:
    [109.0, 325.0]
    Applications:
    Western Blot,IHC (Paraffin)
    Host:
    Mouse
    Immunogen:
    This NANOG monoclonal antibody is generated from mouse immunized with NANOG recombinant protein.
    Size:
    80 ul 400 ul
    Category:
    Antibody
    Antibody Type:
    Primary antibody
    Isotype:
    IgG1
    Preparation:
    This NANOG monoclonal antibody is generated from mouse immunized with NANOG recombinant protein
    Reactivity:
    Human
    Buy from Supplier


    Structured Review

    WuXi AppTec nanog
    The expression of reprogramming transcription factors in 2D- and 3D-grown A549 cells. ( A ) qRT-PCR for the expression of reprogramming transcription factors <t>OCT4,</t> SOX2, <t>NANOG,</t> c-MYC and LIN28 in 2D- and 3D-grown A549 cells. ( B ) Western blotting for expression of OCT4, SOX2 and NANOG in 2D- and 3D-grown A549 cells. ( C ) Gray analysis the result of Figure 3 B. ( D ) qRT-PCR for the expression of reprogramming factor miR-302a expression in 2D- and 3D-grown A549 cells. ( E ) Western blotting for the expression of β-catenin in 2D- and 3D-grown A549 cells. Data are presented as mean ± SE. Experiments were independently repeated at least three times.
    Mouse Monoclonal Antibody Mab IgG1 k
    https://www.bioz.com/result/nanog/product/WuXi AppTec
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nanog - by Bioz Stars, 2020-07
    91/100 stars

    Images

    1) Product Images from "Reprogramming mediated radio-resistance of 3D-grown cancer cells"

    Article Title: Reprogramming mediated radio-resistance of 3D-grown cancer cells

    Journal: Journal of Radiation Research

    doi: 10.1093/jrr/rrv018

    The expression of reprogramming transcription factors in 2D- and 3D-grown A549 cells. ( A ) qRT-PCR for the expression of reprogramming transcription factors OCT4, SOX2, NANOG, c-MYC and LIN28 in 2D- and 3D-grown A549 cells. ( B ) Western blotting for expression of OCT4, SOX2 and NANOG in 2D- and 3D-grown A549 cells. ( C ) Gray analysis the result of Figure 3 B. ( D ) qRT-PCR for the expression of reprogramming factor miR-302a expression in 2D- and 3D-grown A549 cells. ( E ) Western blotting for the expression of β-catenin in 2D- and 3D-grown A549 cells. Data are presented as mean ± SE. Experiments were independently repeated at least three times.
    Figure Legend Snippet: The expression of reprogramming transcription factors in 2D- and 3D-grown A549 cells. ( A ) qRT-PCR for the expression of reprogramming transcription factors OCT4, SOX2, NANOG, c-MYC and LIN28 in 2D- and 3D-grown A549 cells. ( B ) Western blotting for expression of OCT4, SOX2 and NANOG in 2D- and 3D-grown A549 cells. ( C ) Gray analysis the result of Figure 3 B. ( D ) qRT-PCR for the expression of reprogramming factor miR-302a expression in 2D- and 3D-grown A549 cells. ( E ) Western blotting for the expression of β-catenin in 2D- and 3D-grown A549 cells. Data are presented as mean ± SE. Experiments were independently repeated at least three times.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    2) Product Images from "Multi-cycle chemotherapy with the glycolipid-like polymeric micelles evade cancer stem cell enrichment in breast cancer therapy"

    Article Title: Multi-cycle chemotherapy with the glycolipid-like polymeric micelles evade cancer stem cell enrichment in breast cancer therapy

    Journal: Oncotarget

    doi: 10.18632/oncotarget.12159

    Enrichment of CSCs in mammospheres ( A ) Typical photograph of the mammospheres. Scale bar, 200 μm. i for MCF-7 cells and ii for mammospheres. ( B ) CD44+/CD24− cells measured by FCM. ( C ) mRNA fold change of OCT4, SOX2 and Nanog genes after SFM culture ( n = 3). ( D ) Fold change of the three proteins. Above: western-blot analysis; below: semi-quantitative analysis by Image J software. ( E ) CLSM for immunostain of ABCG2 (red). Scale bar, 20 μm. ( F ) Schematic diagram of the mammospheres.
    Figure Legend Snippet: Enrichment of CSCs in mammospheres ( A ) Typical photograph of the mammospheres. Scale bar, 200 μm. i for MCF-7 cells and ii for mammospheres. ( B ) CD44+/CD24− cells measured by FCM. ( C ) mRNA fold change of OCT4, SOX2 and Nanog genes after SFM culture ( n = 3). ( D ) Fold change of the three proteins. Above: western-blot analysis; below: semi-quantitative analysis by Image J software. ( E ) CLSM for immunostain of ABCG2 (red). Scale bar, 20 μm. ( F ) Schematic diagram of the mammospheres.

    Techniques Used: Western Blot, Software, Confocal Laser Scanning Microscopy

    3) Product Images from "Biological characteristics of CD133+ cells in nasopharyngeal carcinoma"

    Article Title: Biological characteristics of CD133+ cells in nasopharyngeal carcinoma

    Journal: Oncology Reports

    doi: 10.3892/or.2013.2408

    Expression of the stem/progenitor markers, Oct3/4, Nanog and Sox2, in CD133 + and CD133 − cells. (A-C) CD133 + cells. (D-F) CD133 − cells. Red, Oct3/4, Nanog and Sox2 staining; blue, 4′,6-diamidino-2-phenylindole (DAPI).
    Figure Legend Snippet: Expression of the stem/progenitor markers, Oct3/4, Nanog and Sox2, in CD133 + and CD133 − cells. (A-C) CD133 + cells. (D-F) CD133 − cells. Red, Oct3/4, Nanog and Sox2 staining; blue, 4′,6-diamidino-2-phenylindole (DAPI).

    Techniques Used: Expressing, Staining

    Related Articles

    Incubation:

    Article Title: Biological characteristics of CD133+ cells in nasopharyngeal carcinoma
    Article Snippet: .. The cells were then incubated with primary antibodies, including Oct3/4 (Santa Cruz Biotechnology, Inc. Santa Cruz, CA, USA), Nanog (Abgent, USA) and Sox2 (Abcam, UK), in 5% BSA at 4°C for 16 h. The cells were then incubated with fluorescent secondary antibodies diluted with 1% BSA at 37°C for 60 min. .. Finally, the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Qiyun Biotechnology, Co., Ltd., China) (1:200), and the slides were visualised using fluorescence microscopy (IX71; Olympus, Japan).