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1) Product Images from "Class I histone deacetylases are major histone decrotonylases: evidence for critical and broad function of histone crotonylation in transcription"
Article Title: Class I histone deacetylases are major histone decrotonylases: evidence for critical and broad function of histone crotonylation in transcription
Journal: Cell Research
Figure Legend Snippet: Histone crotonylation is enriched in and required for self-renewal of mESCs. (A) WB analysis showing a significantly higher level of histone crotonylation in mESC than in differentiated embryoid bodies (EB). CGR8 mESC were induced to differentiate by suspension culture in dish for 9 days. (B) WB analysis showing induced expression of WT HDAC1 and HDAC1-VRPP in CGR8 cells. HA antibody detected only Dox-induced HA-tagged HDAC1 or HDAC1-VRPP, whereas HDAC1 antibody detected both induced and endogenous HDAC1 proteins. (C) Contrast-phase images of control, WT HDAC1 and HDAC1-VRPP CGR8 colonies cultured for 9 days with or without Dox. (D) WB analysis showing the effect of induced expression of WT HDAC1 and HDAC1-VRPP on the levels of mESC core transcription factors Sox2, Oct4 and Nanog and histone crotonylation and histone acetylation. Note that reduced levels of Sox2, Oct4, Nanog, histone crotonylation and histone acetylation were observed upon 3 days of Dox treatment. (E) Confirmation of induced differentiation upon Dox-induced expression of WT HDAC1 or HDAC1-VRPP by qRT-PCR analysis of indicated differentiation marker genes. (F) Working model illustrating a non-redundant function of histone crotonylation to histone acetylation in transcription. CBP/p300 and MOF catalyze both histone acetylation and crotonylation, which in turn recruit corresponding reader proteins such as BRD4 or DPF2 and AF9, and facilitate transcriptional activation (left panel). Selective decrotonylation by HDCR1-VRPP is sufficient to repress transcription, indicating that histone crotonylation is required for transcriptional activation (right panel).
Techniques Used: Western Blot, Expressing, Cell Culture, Quantitative RT-PCR, Marker, Activation Assay
Article Title: Anti-tumor Activity of Bufalin by Inhibiting c-MET Mediated MEK/ERK and PI3K/AKT Signaling Pathways in Gallbladder Cancer
Article Snippet: Primary antibodies include anti-Bcl-2, anti-Mcl-1, anti-Bax, anti-P21, anti-P27, anti-MMP9 and