nanoflow hplc instrument  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nanoflow hplc instrument
    Nanoflow Hplc Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanoflow hplc instrument/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    nanoflow hplc instrument - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Site-Specific N-Glycan Characterization of Grass Carp Serum IgM
    Article Snippet: .. Trypsin or chymotrypsin digested grass carp IgM de-glycopeptides and glycopeptides were analyzed using nanoflow HPLC instrument (EASY-nLC 1200 system, Thermo Fisher Scientific, USA) coupled on-line to a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, USA) in positive polarity mode. .. Chromatography column was Acclaim PepMap, C18, 50 um × 150 mm, particle size 2 um, 100Å (Thermo Fisher Scientific, USA) with buffer A (95% water and 4.9% acetonitrile with 0.1% FA) and buffer B (90% acetonitrile and 9.9% water with 0.1% FA) at a flow rate of 300 nL/min.

    Liquid Chromatography:

    Article Title: Dynamics of PARKIN-dependent mitochondrial ubiquitylation in induced-neurons and model systems revealed by digital snapshot proteomics
    Article Snippet: .. All spectra were acquired on an Oribtrap Fusion or Lumos mass spectrometer (Thermo Fisher Scientific) coupled to an Easy-nLC 1200 (Thermo Fisher Scientific) ultra-high-pressure liquid chromatography (UHPLC) pump. .. Peptides were separated on a 100 µm inner diameter microcapillary column packed in house with ~35 cm of Accucore150 resin (2.6 µm, 150 Å, ThermoFisher Scientific, San Jose, CA) with a gradient consisting of 5%–28% (ACN, 0.1% FA) over 150 min at ~500 nL/min.

    Mass Spectrometry:

    Article Title: Dynamics of PARKIN-dependent mitochondrial ubiquitylation in induced-neurons and model systems revealed by digital snapshot proteomics
    Article Snippet: .. All spectra were acquired on an Oribtrap Fusion or Lumos mass spectrometer (Thermo Fisher Scientific) coupled to an Easy-nLC 1200 (Thermo Fisher Scientific) ultra-high-pressure liquid chromatography (UHPLC) pump. .. Peptides were separated on a 100 µm inner diameter microcapillary column packed in house with ~35 cm of Accucore150 resin (2.6 µm, 150 Å, ThermoFisher Scientific, San Jose, CA) with a gradient consisting of 5%–28% (ACN, 0.1% FA) over 150 min at ~500 nL/min.

    Article Title: Mechanisms of opening and closing of the bacterial replicative helicase
    Article Snippet: .. Peptides were desalted and concentrated on C18 solid phase extraction material (Empore), loaded onto an EASY-Spray column (Thermo Fisher Scientific ES800: 15 cm ×75 μm ID, PepMap C18, 3 μm) via an EASY-nLC 1200 and gradient-eluted for online ESI–MS and MS/MS analyses with a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific). .. MS/MS analyses of the top eight precursors in each full scan used the following parameters: resolution: 17,500 (at 200 Th); AGC target: 2 × 105 ; maximum injection time: 800 ms; isolation width: 1.4 m/z; normalized collision energy: 24%; charge: 3–7; intensity threshold: 2.5 × 103 ; peptide match: off; dynamic exclusion tolerance: 1500 mmu.

    Article Title: Protein kinase D regulates positive selection of CD4+ thymocytes through phosphorylation of SHP-1
    Article Snippet: .. LC–MS/MS analysis of enriched phosphopeptides was performed on an EASY-nLC 1200 UHPLC (Thermo Fisher Scientific) connected to a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific). ..

    Article Title: Site-Specific N-Glycan Characterization of Grass Carp Serum IgM
    Article Snippet: .. Trypsin or chymotrypsin digested grass carp IgM de-glycopeptides and glycopeptides were analyzed using nanoflow HPLC instrument (EASY-nLC 1200 system, Thermo Fisher Scientific, USA) coupled on-line to a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, USA) in positive polarity mode. .. Chromatography column was Acclaim PepMap, C18, 50 um × 150 mm, particle size 2 um, 100Å (Thermo Fisher Scientific, USA) with buffer A (95% water and 4.9% acetonitrile with 0.1% FA) and buffer B (90% acetonitrile and 9.9% water with 0.1% FA) at a flow rate of 300 nL/min.

    Article Title: Global serum proteomic changes in water buffaloes infected with Fasciola gigantica
    Article Snippet: .. LC-MS/MS analysis Shotgun proteomics analyses were performed using an EASY-nLCTM 1200 UHPLC system (Thermo Fisher Scientific) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) operating in the data-dependent acquisition (DDA) mode. .. A sample volume corresponding to 2 μg of total peptides reconstituted in 0.1% FA was injected onto an Acclaim PepMap100 C18 Nano-Trap column (2 cm × 100 μm, 5 μm; Dionex, Sunnyvale, USA).

    Article Title: Structure of the Core of the Type Three Secretion System Export Apparatus
    Article Snippet: .. The resultant peptides were analysed on an EASY-nLC 1200 (Thermo Fisher Scientific) coupled to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). .. Peptides were loaded onto a 75 μm (ID), 15 cm column packed in-house with reversed-phase ReproSil-Pur 120 C18-AQ 1.9 μm resin (Dr. Maisch GmbH).

    Tandem Mass Spectroscopy:

    Article Title: Mechanisms of opening and closing of the bacterial replicative helicase
    Article Snippet: .. Peptides were desalted and concentrated on C18 solid phase extraction material (Empore), loaded onto an EASY-Spray column (Thermo Fisher Scientific ES800: 15 cm ×75 μm ID, PepMap C18, 3 μm) via an EASY-nLC 1200 and gradient-eluted for online ESI–MS and MS/MS analyses with a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific). .. MS/MS analyses of the top eight precursors in each full scan used the following parameters: resolution: 17,500 (at 200 Th); AGC target: 2 × 105 ; maximum injection time: 800 ms; isolation width: 1.4 m/z; normalized collision energy: 24%; charge: 3–7; intensity threshold: 2.5 × 103 ; peptide match: off; dynamic exclusion tolerance: 1500 mmu.

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  • 88
    Thermo Fisher nanoflow hplc instrument
    Nanoflow Hplc Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanoflow hplc instrument/product/Thermo Fisher
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    nanoflow hplc instrument - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    85
    Thermo Fisher ltq orbitrap velos a nanoflow hplc instrument
    Ltq Orbitrap Velos A Nanoflow Hplc Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap velos a nanoflow hplc instrument/product/Thermo Fisher
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap velos a nanoflow hplc instrument - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

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