nanodrop 8000 spectrophotometer  (Thermo Fisher)


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    Name:
    NanoDrop 8000 Spectrophotometer
    Description:
    Thermo Scientific NanoDrop 8000 is a muti sample micro volume UV Vis spectrophotometer With the NanoDrop 8000 you can measure more samples in less time with the same reliability and patented sample retention technology of the NanoDrop 2000
    Catalog Number:
    nd-8000-gl
    Price:
    None
    Applications:
    Industrial & Applied Science|Spectroscopy Elemental Isotope Analysis
    Category:
    Instruments and Equipment
    Buy from Supplier


    Structured Review

    Thermo Fisher nanodrop 8000 spectrophotometer
    Thermo Scientific NanoDrop 8000 is a muti sample micro volume UV Vis spectrophotometer With the NanoDrop 8000 you can measure more samples in less time with the same reliability and patented sample retention technology of the NanoDrop 2000
    https://www.bioz.com/result/nanodrop 8000 spectrophotometer/product/Thermo Fisher
    Average 90 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    nanodrop 8000 spectrophotometer - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: In Vivo Cellular Tropism of Gorilla Simian Foamy Virus in Blood of Infected Humans
    Article Snippet: We used the Eppendorf Mastercycler realplex gradient detection system and Quantitect SYBR green (Qiagen) in a 20-μl-volume reaction mixture containing 10 μl of SYBR green buffer, 150 nM each primer, and 500 ng of a DNA sample (an approximately 75,000-cell equivalent) quantified by a NanoDrop 8000 spectrophotometer (Nanodrop Technologies). .. To standardize the qPCR, we cloned a 465-bp region that included the PCR target sequence from one primary isolate (BAK74, a gorilla strain of SFV) into a PCR cloning plasmid using a TOPO TA cloning kit (Invitrogen) and diluted it in 500 ng of human genomic DNA (Promega).

    Amplification:

    Article Title: PPAR agonists regulate brain gene expression: Relationship to their effects on ethanol consumption
    Article Snippet: Total RNA was quantified on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific Inc., Rockford, IL) and assessed for quality using the Agilent TapeStation (Agilent Technologies, Inc., Santa Clara, CA). .. RNA was amplified and biotin-labeled using the Illumina® TotalPrepTM RNA Amplification kit (Ambion, Austin, TX), and aliquots of labeled cRNA were sent to the Yale Center for Genome Analysis (West Haven, CT), where they were hybridized to Illumina® MouseWG-6 v2 Expression BeadChips (Illumina, Inc., San Diego, CA).

    Polymerase Chain Reaction:

    Article Title: In Vivo Cellular Tropism of Gorilla Simian Foamy Virus in Blood of Infected Humans
    Article Snippet: We used the Eppendorf Mastercycler realplex gradient detection system and Quantitect SYBR green (Qiagen) in a 20-μl-volume reaction mixture containing 10 μl of SYBR green buffer, 150 nM each primer, and 500 ng of a DNA sample (an approximately 75,000-cell equivalent) quantified by a NanoDrop 8000 spectrophotometer (Nanodrop Technologies). .. To standardize the qPCR, we cloned a 465-bp region that included the PCR target sequence from one primary isolate (BAK74, a gorilla strain of SFV) into a PCR cloning plasmid using a TOPO TA cloning kit (Invitrogen) and diluted it in 500 ng of human genomic DNA (Promega).

    Article Title: Measuring Bovine γδ T Cell Function at the Site of Mycobacterium bovis Infection
    Article Snippet: The RNA concentration in each sample was measured by using a NanoDrop 8000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). .. Quantitative real-time PCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems).

    Article Title: Isolation and molecular identification of Mycoplasma equigenitalium from equine genital tracts in northern India
    Article Snippet: Sample preparation for PCR analysis Bacterial strains Genomic DNAs from bacterial cultures were prepared as described by Garcia et al. (1988) . .. Concentrations of genomic DNA isolated from different bacterial strains were measured using a NanoDrop-8000 spectrophotometer (Thermo Fisher scientific, USA) and estimated to be in the range of 1.8-4.6 μg/ml, with the average of 2.56 μg/ml.

    TA Cloning:

    Article Title: In Vivo Cellular Tropism of Gorilla Simian Foamy Virus in Blood of Infected Humans
    Article Snippet: We used the Eppendorf Mastercycler realplex gradient detection system and Quantitect SYBR green (Qiagen) in a 20-μl-volume reaction mixture containing 10 μl of SYBR green buffer, 150 nM each primer, and 500 ng of a DNA sample (an approximately 75,000-cell equivalent) quantified by a NanoDrop 8000 spectrophotometer (Nanodrop Technologies). .. To standardize the qPCR, we cloned a 465-bp region that included the PCR target sequence from one primary isolate (BAK74, a gorilla strain of SFV) into a PCR cloning plasmid using a TOPO TA cloning kit (Invitrogen) and diluted it in 500 ng of human genomic DNA (Promega).

    Construct:

    Article Title: RNA-binding profiles of Drosophila CPEB proteins Orb and Orb2
    Article Snippet: Paragraph title: Generation of DNA constructs . ... The supernatant was moved to another tube, and the RNA concentration was assessed at A260/A280 nm with a NanoDrop 8000 spectrophotometer (Thermo Scientific).

    SYBR Green Assay:

    Article Title: In Vivo Cellular Tropism of Gorilla Simian Foamy Virus in Blood of Infected Humans
    Article Snippet: .. We used the Eppendorf Mastercycler realplex gradient detection system and Quantitect SYBR green (Qiagen) in a 20-μl-volume reaction mixture containing 10 μl of SYBR green buffer, 150 nM each primer, and 500 ng of a DNA sample (an approximately 75,000-cell equivalent) quantified by a NanoDrop 8000 spectrophotometer (Nanodrop Technologies). ..

    Article Title: Measuring Bovine γδ T Cell Function at the Site of Mycobacterium bovis Infection
    Article Snippet: The RNA concentration in each sample was measured by using a NanoDrop 8000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). .. Quantitative real-time PCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems).

    Microarray:

    Article Title: PPAR agonists regulate brain gene expression: Relationship to their effects on ethanol consumption
    Article Snippet: Paragraph title: 2.5 RNA preparation and microarray hybridization ... Total RNA was quantified on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific Inc., Rockford, IL) and assessed for quality using the Agilent TapeStation (Agilent Technologies, Inc., Santa Clara, CA).

    Article Title: Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages
    Article Snippet: Paragraph title: Microarray hybridization and data analysis ... Integrity of the RNA was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies) and purity/concentration was determined by NanoDrop 8000 Spectrophotometer (Thermo Scientific).

    Incubation:

    Article Title: RNA-binding profiles of Drosophila CPEB proteins Orb and Orb2
    Article Snippet: Subsequently 1 μL of rDNase I was added, and incubation was continued for another 30 min. An 0.2 volume (10 μL) of DNase Inactivation Reagent was added to stop the reaction, mixed for 2 min at room temperature, and centrifuged for 2 min at 5,000 rpm (Eppendorf centrifuge 5418R) at room temperature. .. The supernatant was moved to another tube, and the RNA concentration was assessed at A260/A280 nm with a NanoDrop 8000 spectrophotometer (Thermo Scientific).

    Article Title: Isolation and molecular identification of Mycoplasma equigenitalium from equine genital tracts in northern India
    Article Snippet: After the addition of sodium dodecyl sulfate (SDS) to a final concentration of 0.5%, the DNA was incubated with 0.1 mg of proteinase-K per ml at 55°C overnight. .. Concentrations of genomic DNA isolated from different bacterial strains were measured using a NanoDrop-8000 spectrophotometer (Thermo Fisher scientific, USA) and estimated to be in the range of 1.8-4.6 μg/ml, with the average of 2.56 μg/ml.

    Expressing:

    Article Title: PPAR agonists regulate brain gene expression: Relationship to their effects on ethanol consumption
    Article Snippet: Total RNA was quantified on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific Inc., Rockford, IL) and assessed for quality using the Agilent TapeStation (Agilent Technologies, Inc., Santa Clara, CA). .. RNA was amplified and biotin-labeled using the Illumina® TotalPrepTM RNA Amplification kit (Ambion, Austin, TX), and aliquots of labeled cRNA were sent to the Yale Center for Genome Analysis (West Haven, CT), where they were hybridized to Illumina® MouseWG-6 v2 Expression BeadChips (Illumina, Inc., San Diego, CA).

    Article Title: Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages
    Article Snippet: Integrity of the RNA was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies) and purity/concentration was determined by NanoDrop 8000 Spectrophotometer (Thermo Scientific). .. Agilent mouse 8 × 60 k array hybridizations were performed by UCLA Clinical Microarray Core following standard Agilent Expression Analysis protocol ( ).

    BIA-KA:

    Article Title: Variants at chromosome 10q26 locus and the expression of HTRA1 in the retina
    Article Snippet: The quality and quantity of RNA were monitored by a Bioanalyzer 2100 (Agilent) and a NanoDrop 8000 Spectrophotometer (Thermo). .. The concentration of total protein was measured using a BCA protein assay kit (Thermo).

    Hybridization:

    Article Title: PPAR agonists regulate brain gene expression: Relationship to their effects on ethanol consumption
    Article Snippet: Paragraph title: 2.5 RNA preparation and microarray hybridization ... Total RNA was quantified on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific Inc., Rockford, IL) and assessed for quality using the Agilent TapeStation (Agilent Technologies, Inc., Santa Clara, CA).

    Article Title: Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages
    Article Snippet: Paragraph title: Microarray hybridization and data analysis ... Integrity of the RNA was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies) and purity/concentration was determined by NanoDrop 8000 Spectrophotometer (Thermo Scientific).

    Electron Microscopy:

    Article Title: Antioxidant and Anti-Inflammatory Properties of a Thuja occidentalis Mother Tincture for the Treatment of Ulcerative Colitis
    Article Snippet: Transmission electron microscopy (TEM) analysis: Colon biopsies were fixed in glutaraldehyde, washed in 0.1 M phosphate buffer, and post-fixed in 2% osmic acid in 0.15 M phosphate buffer. .. The quantity and quality of extracted RNA were evaluated using the NanoDrop 8000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Transmission Assay:

    Article Title: Antioxidant and Anti-Inflammatory Properties of a Thuja occidentalis Mother Tincture for the Treatment of Ulcerative Colitis
    Article Snippet: Ultra-sections of 60 nm thickness were made with a Leica EM UC7 ultramicrotome (Leica Microsystems GmbH, Wetzler, Germany) and then analyzed on an electron microscope with a Tecnai 12 Biotwin transmission (FEI company, Eindhoven, The Netherlands). .. The quantity and quality of extracted RNA were evaluated using the NanoDrop 8000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Antioxidant and Anti-Inflammatory Properties of a Thuja occidentalis Mother Tincture for the Treatment of Ulcerative Colitis
    Article Snippet: Real-time polymerase chain reaction (RT-PCR) analysis: Samples of intestinal tissue were stored in RNA solution (Thermo Scientific, Waltham, MA, USA) at −80 °C until analysis. .. The quantity and quality of extracted RNA were evaluated using the NanoDrop 8000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Quantitative RT-PCR:

    Article Title: Downregulation of PIM1 regulates glycolysis and suppresses tumor progression in gallbladder cancer
    Article Snippet: Paragraph title: Quantitative real-time PCR (qRT-PCR) ... Briefly, NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) was used to detect the concentration of RNA.

    Recombinant:

    Article Title: RNA-binding profiles of Drosophila CPEB proteins Orb and Orb2
    Article Snippet: One microliter of recombinant DNase I (rDNase I) and 5 μL of 10× rDNase buffer were added to DNA and incubated for 30 min at 37 °C. .. The supernatant was moved to another tube, and the RNA concentration was assessed at A260/A280 nm with a NanoDrop 8000 spectrophotometer (Thermo Scientific).

    Transmission Electron Microscopy:

    Article Title: Antioxidant and Anti-Inflammatory Properties of a Thuja occidentalis Mother Tincture for the Treatment of Ulcerative Colitis
    Article Snippet: Transmission electron microscopy (TEM) analysis: Colon biopsies were fixed in glutaraldehyde, washed in 0.1 M phosphate buffer, and post-fixed in 2% osmic acid in 0.15 M phosphate buffer. .. The quantity and quality of extracted RNA were evaluated using the NanoDrop 8000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    DNA Extraction:

    Article Title: The Predictive Potentiality of Salivary Microbiome for the Recurrence of Early Childhood Caries
    Article Snippet: Total genomic DNA extraction was performed using QIAamp DNA mini kit (Qiagen, Hilden, Germany) according to manufacturer's instructions. .. Concentration and purity testing of the DNA was performed using a NanoDrop 8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).

    Article Title: High Throughput Sequencing and Network Analysis Disentangle the Microbial Communities of Ticks and Hosts Within and Between Ecosystems
    Article Snippet: One extraction blank, corresponding to an extraction without sample tissue, was systematically added to each of the DNA extraction microplates. .. DNA was quantified with a NanoDrop 8000 spectrophotometer (Thermo Scientific, Waltham, MA, United States), to confirm the presence of a minimum of 10 ng/μl of DNA in each sample.

    Article Title: Associations between stress and depression symptom profiles vary according to serotonin transporter polymorphism in rural Australians
    Article Snippet: Paragraph title: DNA extraction ... Presence of intact, high-molecular-weight DNA was confirmed through gel electrophoresis and quantified using a NanoDrop 8000 spectrophotometer (NanoDrop, ThermoFisher, Melbourne, Australia).

    Nucleic Acid Electrophoresis:

    Article Title: Associations between stress and depression symptom profiles vary according to serotonin transporter polymorphism in rural Australians
    Article Snippet: .. Presence of intact, high-molecular-weight DNA was confirmed through gel electrophoresis and quantified using a NanoDrop 8000 spectrophotometer (NanoDrop, ThermoFisher, Melbourne, Australia). .. 5HTTLPR genotyping for short and long alleles into s-present (ie, ss, sl) versus s-absent (ie, ll) was carried out using polymerase chain reaction (PCR), as described by Wendland et al.

    In Vivo:

    Article Title: Antioxidant and Anti-Inflammatory Properties of a Thuja occidentalis Mother Tincture for the Treatment of Ulcerative Colitis
    Article Snippet: Paragraph title: 2.4. In Vivo Antioxidant and Anti-Inflammatory Capacity Assessment ... The quantity and quality of extracted RNA were evaluated using the NanoDrop 8000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Isolation:

    Article Title: Expression of the Placental Transcriptome in Maternal Nutrient Reduction in Baboons Is Dependent on Fetal Sex 1Expression of the Placental Transcriptome in Maternal Nutrient Reduction in Baboons Is Dependent on Fetal Sex 1 2Expression of the Placental Transcriptome in Maternal Nutrient Reduction in Baboons Is Dependent on Fetal Sex 1 2 3
    Article Snippet: Paragraph title: RNA isolation from tissue. ... RNA quality was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) and RNA concentrations confirmed by quantitation using a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific).

    Article Title: Antioxidant and Anti-Inflammatory Properties of a Thuja occidentalis Mother Tincture for the Treatment of Ulcerative Colitis
    Article Snippet: The tissue was homogenized, and the RNA was extracted using the SV Total RNA Isolation System kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions. .. The quantity and quality of extracted RNA were evaluated using the NanoDrop 8000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Article Title: PPAR agonists regulate brain gene expression: Relationship to their effects on ethanol consumption
    Article Snippet: We extracted RNA using the MagMAX-96 Total RNA Isolation Kit (Life Technologies, Grand Island, NY). .. Total RNA was quantified on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific Inc., Rockford, IL) and assessed for quality using the Agilent TapeStation (Agilent Technologies, Inc., Santa Clara, CA).

    Article Title: Measuring Bovine γδ T Cell Function at the Site of Mycobacterium bovis Infection
    Article Snippet: Total RNA was extracted using the RNeasy Mini RNA Isolation Kit (Qiagen, Germantown, MD, USA), according to manufacturer’s instructions. .. The RNA concentration in each sample was measured by using a NanoDrop 8000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA).

    Article Title: Isolation and molecular identification of Mycoplasma equigenitalium from equine genital tracts in northern India
    Article Snippet: .. Concentrations of genomic DNA isolated from different bacterial strains were measured using a NanoDrop-8000 spectrophotometer (Thermo Fisher scientific, USA) and estimated to be in the range of 1.8-4.6 μg/ml, with the average of 2.56 μg/ml. .. Genital swabs For sample preparation, swabs were suspended in 200 μL sterile phosphate buffered saline (PBS).

    Article Title: MicroRNA Expression Aids the Preoperative Diagnosis of Pancreatic Ductal Adenocarcinoma
    Article Snippet: RNA was extracted using the Ambion RecoverAll Total Nucleic Acid Isolation kit (Applied Biosystems, Foster City, California). .. RNA was captured on a glass-fiber filter and eluted using 60 μL of nuclease-free water after ethanol wash. Total RNA concentrations were measured with the NanoDrop 8000 spectrophotometer (NanoDrop, Wilmington, DE).

    Negative Control:

    Article Title: The Predictive Potentiality of Salivary Microbiome for the Recurrence of Early Childhood Caries
    Article Snippet: Concentration and purity testing of the DNA was performed using a NanoDrop 8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. The integrity of bacterial genomic DNA was checked by 1% agarose gel electrophoresis and a negative control only with buffer was used.

    Labeling:

    Article Title: PPAR agonists regulate brain gene expression: Relationship to their effects on ethanol consumption
    Article Snippet: Total RNA was quantified on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific Inc., Rockford, IL) and assessed for quality using the Agilent TapeStation (Agilent Technologies, Inc., Santa Clara, CA). .. RNA was amplified and biotin-labeled using the Illumina® TotalPrepTM RNA Amplification kit (Ambion, Austin, TX), and aliquots of labeled cRNA were sent to the Yale Center for Genome Analysis (West Haven, CT), where they were hybridized to Illumina® MouseWG-6 v2 Expression BeadChips (Illumina, Inc., San Diego, CA).

    Sequencing:

    Article Title: In Vivo Cellular Tropism of Gorilla Simian Foamy Virus in Blood of Infected Humans
    Article Snippet: We used the Eppendorf Mastercycler realplex gradient detection system and Quantitect SYBR green (Qiagen) in a 20-μl-volume reaction mixture containing 10 μl of SYBR green buffer, 150 nM each primer, and 500 ng of a DNA sample (an approximately 75,000-cell equivalent) quantified by a NanoDrop 8000 spectrophotometer (Nanodrop Technologies). .. To standardize the qPCR, we cloned a 465-bp region that included the PCR target sequence from one primary isolate (BAK74, a gorilla strain of SFV) into a PCR cloning plasmid using a TOPO TA cloning kit (Invitrogen) and diluted it in 500 ng of human genomic DNA (Promega).

    Article Title: Downregulation of PIM1 regulates glycolysis and suppresses tumor progression in gallbladder cancer
    Article Snippet: Briefly, NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) was used to detect the concentration of RNA. .. Additionally, total RNA was reverse transcribed with TransScript First-Strand cDNA Synthesis SuperMix (TransGen, Beijing, China). qRT-PCR was performed on 7500 sequence detection system.

    Article Title: Transcriptomic Analyses Reveal Novel Genes with Sexually Dimorphic Expression in Yellow Catfish (Pelteobagrus fulvidraco) Brain
    Article Snippet: The concentration and integrity of RNA were examined using Thermo Scientific™ NanoDrop™ 8000 Spectrophotometer and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. Equal amounts of the high-quality RNA samples from brain tissue were then pooled together for cDNA synthesis and sequencing.

    Microscopy:

    Article Title: Antioxidant and Anti-Inflammatory Properties of a Thuja occidentalis Mother Tincture for the Treatment of Ulcerative Colitis
    Article Snippet: Ultra-sections of 60 nm thickness were made with a Leica EM UC7 ultramicrotome (Leica Microsystems GmbH, Wetzler, Germany) and then analyzed on an electron microscope with a Tecnai 12 Biotwin transmission (FEI company, Eindhoven, The Netherlands). .. The quantity and quality of extracted RNA were evaluated using the NanoDrop 8000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    cDNA Library Assay:

    Article Title: RNA-binding profiles of Drosophila CPEB proteins Orb and Orb2
    Article Snippet: The supernatant was moved to another tube, and the RNA concentration was assessed at A260/A280 nm with a NanoDrop 8000 spectrophotometer (Thermo Scientific). .. A cDNA library was prepared by reverse transcription using an oligo(dT)20 primer (Invitrogen, 18418-020).

    Sample Prep:

    Article Title: The Predictive Potentiality of Salivary Microbiome for the Recurrence of Early Childhood Caries
    Article Snippet: Paragraph title: Saliva Sampling and Total Genomic DNA Sample Preparation ... Concentration and purity testing of the DNA was performed using a NanoDrop 8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).

    Article Title: Isolation and molecular identification of Mycoplasma equigenitalium from equine genital tracts in northern India
    Article Snippet: Sample preparation for PCR analysis Bacterial strains Genomic DNAs from bacterial cultures were prepared as described by Garcia et al. (1988) . .. Concentrations of genomic DNA isolated from different bacterial strains were measured using a NanoDrop-8000 spectrophotometer (Thermo Fisher scientific, USA) and estimated to be in the range of 1.8-4.6 μg/ml, with the average of 2.56 μg/ml.

    Plasmid Preparation:

    Article Title: In Vivo Cellular Tropism of Gorilla Simian Foamy Virus in Blood of Infected Humans
    Article Snippet: We used the Eppendorf Mastercycler realplex gradient detection system and Quantitect SYBR green (Qiagen) in a 20-μl-volume reaction mixture containing 10 μl of SYBR green buffer, 150 nM each primer, and 500 ng of a DNA sample (an approximately 75,000-cell equivalent) quantified by a NanoDrop 8000 spectrophotometer (Nanodrop Technologies). .. To standardize the qPCR, we cloned a 465-bp region that included the PCR target sequence from one primary isolate (BAK74, a gorilla strain of SFV) into a PCR cloning plasmid using a TOPO TA cloning kit (Invitrogen) and diluted it in 500 ng of human genomic DNA (Promega).

    Software:

    Article Title: Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages
    Article Snippet: Integrity of the RNA was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies) and purity/concentration was determined by NanoDrop 8000 Spectrophotometer (Thermo Scientific). .. The acquisition of array image was undertaken by Agilent Scan Control and Feature Extraction 10.7 software.

    Real-time Polymerase Chain Reaction:

    Article Title: Antioxidant and Anti-Inflammatory Properties of a Thuja occidentalis Mother Tincture for the Treatment of Ulcerative Colitis
    Article Snippet: Real-time polymerase chain reaction (RT-PCR) analysis: Samples of intestinal tissue were stored in RNA solution (Thermo Scientific, Waltham, MA, USA) at −80 °C until analysis. .. The quantity and quality of extracted RNA were evaluated using the NanoDrop 8000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Article Title: Downregulation of PIM1 regulates glycolysis and suppresses tumor progression in gallbladder cancer
    Article Snippet: Paragraph title: Quantitative real-time PCR (qRT-PCR) ... Briefly, NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) was used to detect the concentration of RNA.

    Article Title: Measuring Bovine γδ T Cell Function at the Site of Mycobacterium bovis Infection
    Article Snippet: Paragraph title: 2.9 Real-time PCR ... The RNA concentration in each sample was measured by using a NanoDrop 8000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA).

    RNA Extraction:

    Article Title: Transcriptomic Analyses Reveal Novel Genes with Sexually Dimorphic Expression in Yellow Catfish (Pelteobagrus fulvidraco) Brain
    Article Snippet: Paragraph title: RNA Extraction ... The concentration and integrity of RNA were examined using Thermo Scientific™ NanoDrop™ 8000 Spectrophotometer and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: MicroRNA Expression Aids the Preoperative Diagnosis of Pancreatic Ductal Adenocarcinoma
    Article Snippet: Paragraph title: RNA Extraction ... RNA was captured on a glass-fiber filter and eluted using 60 μL of nuclease-free water after ethanol wash. Total RNA concentrations were measured with the NanoDrop 8000 spectrophotometer (NanoDrop, Wilmington, DE).

    Agarose Gel Electrophoresis:

    Article Title: The Predictive Potentiality of Salivary Microbiome for the Recurrence of Early Childhood Caries
    Article Snippet: Concentration and purity testing of the DNA was performed using a NanoDrop 8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. The integrity of bacterial genomic DNA was checked by 1% agarose gel electrophoresis and a negative control only with buffer was used.

    Quantitation Assay:

    Article Title: Expression of the Placental Transcriptome in Maternal Nutrient Reduction in Baboons Is Dependent on Fetal Sex 1Expression of the Placental Transcriptome in Maternal Nutrient Reduction in Baboons Is Dependent on Fetal Sex 1 2Expression of the Placental Transcriptome in Maternal Nutrient Reduction in Baboons Is Dependent on Fetal Sex 1 2 3
    Article Snippet: .. RNA quality was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) and RNA concentrations confirmed by quantitation using a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific). ..

    Spectrophotometry:

    Article Title: Expression of the Placental Transcriptome in Maternal Nutrient Reduction in Baboons Is Dependent on Fetal Sex 1Expression of the Placental Transcriptome in Maternal Nutrient Reduction in Baboons Is Dependent on Fetal Sex 1 2Expression of the Placental Transcriptome in Maternal Nutrient Reduction in Baboons Is Dependent on Fetal Sex 1 2 3
    Article Snippet: .. RNA quality was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) and RNA concentrations confirmed by quantitation using a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific). ..

    Article Title: The Predictive Potentiality of Salivary Microbiome for the Recurrence of Early Childhood Caries
    Article Snippet: .. Concentration and purity testing of the DNA was performed using a NanoDrop 8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. The integrity of bacterial genomic DNA was checked by 1% agarose gel electrophoresis and a negative control only with buffer was used.

    Article Title: Antioxidant and Anti-Inflammatory Properties of a Thuja occidentalis Mother Tincture for the Treatment of Ulcerative Colitis
    Article Snippet: .. The quantity and quality of extracted RNA were evaluated using the NanoDrop 8000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA). .. Then, 2 μg total RNA was reverse transcribed into cDNA using the First Strand cDNA Synthesis Kit (Thermo Scientific, Waltham, MA, USA).

    Article Title: RNA-binding profiles of Drosophila CPEB proteins Orb and Orb2
    Article Snippet: .. The supernatant was moved to another tube, and the RNA concentration was assessed at A260/A280 nm with a NanoDrop 8000 spectrophotometer (Thermo Scientific). .. A cDNA library was prepared by reverse transcription using an oligo(dT)20 primer (Invitrogen, 18418-020).

    Article Title: Variants at chromosome 10q26 locus and the expression of HTRA1 in the retina
    Article Snippet: .. The quality and quantity of RNA were monitored by a Bioanalyzer 2100 (Agilent) and a NanoDrop 8000 Spectrophotometer (Thermo). .. The concentration of total protein was measured using a BCA protein assay kit (Thermo).

    Article Title: In Vivo Cellular Tropism of Gorilla Simian Foamy Virus in Blood of Infected Humans
    Article Snippet: .. We used the Eppendorf Mastercycler realplex gradient detection system and Quantitect SYBR green (Qiagen) in a 20-μl-volume reaction mixture containing 10 μl of SYBR green buffer, 150 nM each primer, and 500 ng of a DNA sample (an approximately 75,000-cell equivalent) quantified by a NanoDrop 8000 spectrophotometer (Nanodrop Technologies). ..

    Article Title: Downregulation of PIM1 regulates glycolysis and suppresses tumor progression in gallbladder cancer
    Article Snippet: .. Briefly, NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) was used to detect the concentration of RNA. .. Additionally, total RNA was reverse transcribed with TransScript First-Strand cDNA Synthesis SuperMix (TransGen, Beijing, China). qRT-PCR was performed on 7500 sequence detection system.

    Article Title: PPAR agonists regulate brain gene expression: Relationship to their effects on ethanol consumption
    Article Snippet: .. Total RNA was quantified on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific Inc., Rockford, IL) and assessed for quality using the Agilent TapeStation (Agilent Technologies, Inc., Santa Clara, CA). ..

    Article Title: Transcriptomic Analyses Reveal Novel Genes with Sexually Dimorphic Expression in Yellow Catfish (Pelteobagrus fulvidraco) Brain
    Article Snippet: .. The concentration and integrity of RNA were examined using Thermo Scientific™ NanoDrop™ 8000 Spectrophotometer and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). ..

    Article Title: Measuring Bovine γδ T Cell Function at the Site of Mycobacterium bovis Infection
    Article Snippet: .. The RNA concentration in each sample was measured by using a NanoDrop 8000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). .. Total eluted RNA was reverse transcribed into cDNA using Random Primers and Superscript III Reverse Transcriptase per the manufacturer’s instructions (Invitrogen, Life Technologies).

    Article Title: Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages
    Article Snippet: .. Integrity of the RNA was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies) and purity/concentration was determined by NanoDrop 8000 Spectrophotometer (Thermo Scientific). .. Agilent mouse 8 × 60 k array hybridizations were performed by UCLA Clinical Microarray Core following standard Agilent Expression Analysis protocol ( ).

    Article Title: Associations between stress and depression symptom profiles vary according to serotonin transporter polymorphism in rural Australians
    Article Snippet: .. Presence of intact, high-molecular-weight DNA was confirmed through gel electrophoresis and quantified using a NanoDrop 8000 spectrophotometer (NanoDrop, ThermoFisher, Melbourne, Australia). .. 5HTTLPR genotyping for short and long alleles into s-present (ie, ss, sl) versus s-absent (ie, ll) was carried out using polymerase chain reaction (PCR), as described by Wendland et al.

    Article Title: Isolation and molecular identification of Mycoplasma equigenitalium from equine genital tracts in northern India
    Article Snippet: .. Concentrations of genomic DNA isolated from different bacterial strains were measured using a NanoDrop-8000 spectrophotometer (Thermo Fisher scientific, USA) and estimated to be in the range of 1.8-4.6 μg/ml, with the average of 2.56 μg/ml. .. Genital swabs For sample preparation, swabs were suspended in 200 μL sterile phosphate buffered saline (PBS).

    Sampling:

    Article Title: The Predictive Potentiality of Salivary Microbiome for the Recurrence of Early Childhood Caries
    Article Snippet: Paragraph title: Saliva Sampling and Total Genomic DNA Sample Preparation ... Concentration and purity testing of the DNA was performed using a NanoDrop 8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).

    Concentration Assay:

    Article Title: The Predictive Potentiality of Salivary Microbiome for the Recurrence of Early Childhood Caries
    Article Snippet: .. Concentration and purity testing of the DNA was performed using a NanoDrop 8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. The integrity of bacterial genomic DNA was checked by 1% agarose gel electrophoresis and a negative control only with buffer was used.

    Article Title: RNA-binding profiles of Drosophila CPEB proteins Orb and Orb2
    Article Snippet: .. The supernatant was moved to another tube, and the RNA concentration was assessed at A260/A280 nm with a NanoDrop 8000 spectrophotometer (Thermo Scientific). .. A cDNA library was prepared by reverse transcription using an oligo(dT)20 primer (Invitrogen, 18418-020).

    Article Title: Variants at chromosome 10q26 locus and the expression of HTRA1 in the retina
    Article Snippet: The quality and quantity of RNA were monitored by a Bioanalyzer 2100 (Agilent) and a NanoDrop 8000 Spectrophotometer (Thermo). .. The concentration of total protein was measured using a BCA protein assay kit (Thermo).

    Article Title: Downregulation of PIM1 regulates glycolysis and suppresses tumor progression in gallbladder cancer
    Article Snippet: .. Briefly, NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) was used to detect the concentration of RNA. .. Additionally, total RNA was reverse transcribed with TransScript First-Strand cDNA Synthesis SuperMix (TransGen, Beijing, China). qRT-PCR was performed on 7500 sequence detection system.

    Article Title: Transcriptomic Analyses Reveal Novel Genes with Sexually Dimorphic Expression in Yellow Catfish (Pelteobagrus fulvidraco) Brain
    Article Snippet: .. The concentration and integrity of RNA were examined using Thermo Scientific™ NanoDrop™ 8000 Spectrophotometer and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). ..

    Article Title: Measuring Bovine γδ T Cell Function at the Site of Mycobacterium bovis Infection
    Article Snippet: .. The RNA concentration in each sample was measured by using a NanoDrop 8000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). .. Total eluted RNA was reverse transcribed into cDNA using Random Primers and Superscript III Reverse Transcriptase per the manufacturer’s instructions (Invitrogen, Life Technologies).

    Article Title: Isolation and molecular identification of Mycoplasma equigenitalium from equine genital tracts in northern India
    Article Snippet: After the addition of sodium dodecyl sulfate (SDS) to a final concentration of 0.5%, the DNA was incubated with 0.1 mg of proteinase-K per ml at 55°C overnight. .. Concentrations of genomic DNA isolated from different bacterial strains were measured using a NanoDrop-8000 spectrophotometer (Thermo Fisher scientific, USA) and estimated to be in the range of 1.8-4.6 μg/ml, with the average of 2.56 μg/ml.

    Lysis:

    Article Title: Isolation and molecular identification of Mycoplasma equigenitalium from equine genital tracts in northern India
    Article Snippet: Concentrations of genomic DNA isolated from different bacterial strains were measured using a NanoDrop-8000 spectrophotometer (Thermo Fisher scientific, USA) and estimated to be in the range of 1.8-4.6 μg/ml, with the average of 2.56 μg/ml. .. The 200 μL suspensions and 100 μL semen or pre-ejaculatory fluid samples were centrifuged at 14,000 g for 20 min. Pellets were re-suspended in 385 μL of a lysis buffer containing 20 mM Tris-HCl (pH = 8.0), 1 mM ethylene diamine tetra acetate (EDTA), 30 mM dithiothreitol, 0.5% sodium dodecyl sulphate (SDS) and 0.4 mg proteinase K/ml.

    Hood:

    Article Title: High Throughput Sequencing and Network Analysis Disentangle the Microbial Communities of Ticks and Hosts Within and Between Ecosystems
    Article Snippet: In order to avoid cross-contamination of samples with environmental bacterial DNA, pre-PCR laboratory manipulations were conducted with filter tips under a sterile hood in a DNA-free room. .. DNA was quantified with a NanoDrop 8000 spectrophotometer (Thermo Scientific, Waltham, MA, United States), to confirm the presence of a minimum of 10 ng/μl of DNA in each sample.

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  • 90
    Thermo Fisher nanodrop 8000 spectrophotometer
    Nanodrop 8000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodrop 8000 spectrophotometer/product/Thermo Fisher
    Average 90 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    nanodrop 8000 spectrophotometer - by Bioz Stars, 2020-01
    90/100 stars
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    Thermo Scientific NanoDrop 8000 is a muti sample micro volume UV Vis spectrophotometer With the NanoDrop 8000 you can measure more samples in less time with the same reliability and
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