nanodrop 1000 spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nanodrop 1000 spectrometer
    Nanodrop 1000 Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodrop 1000 spectrometer/product/Thermo Fisher
    Average 99 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    nanodrop 1000 spectrometer - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Comparative genomic hybridization and transcriptome analysis with a pan-genome microarray reveal distinctions between JP2 and non-JP2 genotypes of Aggregatibacter actinomycetemcomitans
    Article Snippet: A change to the protocol was made to use a nitrogen gun for drying the arrays after washing (instead of centrifugation or drying solution). .. RNA Yield and Quality was assessed by Nanodrop-1000 spectrometer and Agilent bioanalyzer.

    Article Title: Metabolic reprogramming during neuronal differentiation
    Article Snippet: After centrifugation, the DNA pellet was washed with 70% EtOH, air dried and dissolved in sterile water. .. DNA concentrations were measured using a Nanodrop 1000 spectrometer (Thermo Scientific).

    Amplification:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: The quality of extracted RNA was assessed by checking the 260/280 and 260/230 ratios using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific) and by running samples (500 ng) on a 1.5% Tris-Borate-EDTA (TBE) agarose gel prepared with 20 mM of guanidium isothiocyanate (GITC). .. For cDNA library preparation and RNA Sequencing, RNA (50 ng each) from each sample was reverse transcribed according to the instructions provided in the Ion AmpliSeq Transcriptome Human Gene Expression kit (Revision A.0, Thermo Fisher Scientific) and the generated cDNA was amplified using the Ion AmpliSeq Transcriptome Human Gene Expression core panel (Thermo Fisher Scientific).

    Article Title: Altered Transcription of Murine Genes Induced in the Small Bowel by Administration of Probiotic Strain Lactobacillus rhamnosus HN001
    Article Snippet: A portion comprising regions V1 to V3 of the bacterial 16S rRNA gene was amplified using a two-step protocol similar to that described by Dowd et al. ( ). .. Products were cleaned using Qiagen PCR cleanup columns (Qiagen, Hilden, Germany) and quantified using a Nanodrop 1000 spectrometer.

    Filtration:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: ESPs (45μg) were incubated with 11.3 nmol/μl of Alexa Fluor-488 and excess dye was removed by gel filtration following the instructions in the Alexa Fluor 488 Microscale Protein Labelling Kit (Thermo Fisher Scientific). .. The amounts of labelled proteins were quantified using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific).

    Synthesized:

    Article Title: Pulmonary response after exposure to inhaled nickel hydroxide nanoparticles: short and long-term studies in mice
    Article Snippet: The quality and concentration was determined using a Nanodrop 1000 spectrometer (Thermo Fisher Scientific, Wilmington, DE). .. With primer/probe sets from TaqMan® Gene Expression Assays, the forward and reverse primers were specific to each gene and synthesized by Applied Biosystems from the following mRNA reference sequences: Il-1α ( ); Tnf-α ( ); Mip-2 ( ); and Ccl2 ( ).

    Quantitative RT-PCR:

    Article Title: Pulmonary response after exposure to inhaled nickel hydroxide nanoparticles: short and long-term studies in mice
    Article Snippet: Paragraph title: Quantitative RT-PCR ... The quality and concentration was determined using a Nanodrop 1000 spectrometer (Thermo Fisher Scientific, Wilmington, DE).

    Article Title: Condition-dependent virulence of slow bee paralysis virus in Bombus terrestris: are the impacts of honeybee viruses in wild pollinators underestimated?
    Article Snippet: .. Real-time quantitative RT-PCR RNA extractions from all bee guts in the infection time course were measured on a Nanodrop 1000 spectrometer. ..

    Article Title: Metabolic reprogramming during neuronal differentiation
    Article Snippet: DNA concentrations were measured using a Nanodrop 1000 spectrometer (Thermo Scientific). .. The relative amount of mtDNA was valued by qRT-PCR performed in an ABI PRISM 7000 Sequence Detection System (Applied Biosystem) with SYBR green ready mix (Applied Biosystem) with the following primers: SDHA Fwd 5′-AGACCTTGAATGAGGCTGACTGTG-3′, SDHA Rev 5′-GAGACAAAGTCTGGCGCAACTC-3′, ND5 Fwd 5′-TGATGGTACGGACGAACAGACG-3′, and ND5 Rev 5′-CTGATGGTAGTCATGGGTGGAG-3′

    Real-time Polymerase Chain Reaction:

    Article Title: Pulmonary response after exposure to inhaled nickel hydroxide nanoparticles: short and long-term studies in mice
    Article Snippet: The quality and concentration was determined using a Nanodrop 1000 spectrometer (Thermo Fisher Scientific, Wilmington, DE). .. To quantify relative mRNA levels of selected genes: macrophage inflammatory protein-2 (Mip-2), Ccl2, interleukin 1-alpha (Il-1α), and tumor necrosis factor-alpha (Tnf-α), real-time polymerase chain reaction (PCR) was performed on an Applied Biosystems 7300 Real-Time PCR instrument (Applied Biosystems, Foster City, CA), under the following conditions: 10 min at 95°C followed by 40 cycles at 95°C and 60°C (15 sec and 1 min, respectively).

    Article Title: Condition-dependent virulence of slow bee paralysis virus in Bombus terrestris: are the impacts of honeybee viruses in wild pollinators underestimated?
    Article Snippet: Real-time quantitative RT-PCR RNA extractions from all bee guts in the infection time course were measured on a Nanodrop 1000 spectrometer. .. Two samples fell below 1.8 and were excluded from qPCR analysis.

    Incubation:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: To visualise ESPs interaction with host cells, chamber slides were washed and replenished with fresh DMEM as above, followed by adding labelled ESPs to the wells at 1 or 5 μg/ml and incubation for 2 h or 6 h (37°C, 10% CO2 ). .. The amounts of labelled proteins were quantified using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific).

    Article Title: Roles of MAPK Pathway Activation During Cytokine Induction in BEAS-2B Cells Exposed to Fine World Trade Center (WTC) Dust
    Article Snippet: The lysed cell suspension was mixed with chloroform (200 μl per 1 ml Trizol), vortexed, incubated at room temperature for 2–3 min, and then centrifuged (12,000 × g, 15 min, 4°C). .. The pellet was then solubilized in RNase-free H2 O, heated at 50°C for 10 min, and the concentration of RNA present determined by measuring absorbance at 260 nm in a NanoDrop 1000 spectrometer (Thermo Scientific, Rockford, IL).

    Article Title: Dawn- and dusk-phased circadian transcription rhythms coordinate anabolic and catabolic functions in Neurospora
    Article Snippet: Extraction buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 1.5 mM MgCl2 , 0.1% NP-40, 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 μg/ml leupeptin, 1 μg/ml pepstatin A) was mixed with 500 μl ground mycelia and incubated on ice for approximately 30 minutes with frequent vortexing. .. Protein concentration was determined by NanoDrop 1000 spectrometer.

    Article Title: Associations of LEP, CRH, ICAM-1, and LINE-1 methylation, measured in saliva, with waist circumference, body mass index, and percent body fat in mid-childhood
    Article Snippet: Mixtures were incubated in a thermocycler with 16 thermal cycles at 95 °C for 30 s and 50 °C for 1 h. Bisulfite-converted DNA samples were loaded onto 96-column plates provided in the kit for desulfonation and purification. .. The concentration of eluted DNA was measured using a NanoDrop 1000 spectrometer.

    Article Title: Metabolic reprogramming during neuronal differentiation
    Article Snippet: Then samples were incubated for 10 min at 95 °C to inhibit the Proteinase K and centrifuged at 8000 r.p.m. for 15 min at 4 °C. .. DNA concentrations were measured using a Nanodrop 1000 spectrometer (Thermo Scientific).

    Activity Assay:

    Article Title: Dissecting and Reconstructing Synergism
    Article Snippet: Using the filter paper unit (FPU) assay as recommended by the IUPAC , the activity of the complete cellulase system was determined as 2.1 FPU/mg. .. The concentration of pure enzyme preparations was determined via their intrinsic UV absorbance at a wavelength of 280 nm using a Nanodrop 1000 Spectrometer (Thermo Fisher Scientific Inc., Walham, MA).

    Article Title: Dawn- and dusk-phased circadian transcription rhythms coordinate anabolic and catabolic functions in Neurospora
    Article Snippet: Protein concentration was determined by NanoDrop 1000 spectrometer. .. Cellulase activity was calculated by using Azo-CM-Cellulose (Megazyme, Bray, Ireland) according to the manufacturer's protocol with minor modifications.

    Expressing:

    Article Title: MicroRNA-150 Is a Potential Biomarker of HIV/AIDS Disease Progression and Therapy
    Article Snippet: The immobilized RNA was eluted in 50 µl of RNAse-free water and was quantified using a NanoDrop 1000 spectrometer (Thermo Scientific, Wimington, DE, USA). .. The efficiency of small RNA isolation was monitored by the amount of spiked-in C. elegans miR-39 recovered and was used as internal control for normalizing the expression of miR-146b-5p and miR-150.

    Article Title: Pulmonary response after exposure to inhaled nickel hydroxide nanoparticles: short and long-term studies in mice
    Article Snippet: The quality and concentration was determined using a Nanodrop 1000 spectrometer (Thermo Fisher Scientific, Wilmington, DE). .. With primer/probe sets from TaqMan® Gene Expression Assays, the forward and reverse primers were specific to each gene and synthesized by Applied Biosystems from the following mRNA reference sequences: Il-1α ( ); Tnf-α ( ); Mip-2 ( ); and Ccl2 ( ).

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: The quality of extracted RNA was assessed by checking the 260/280 and 260/230 ratios using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific) and by running samples (500 ng) on a 1.5% Tris-Borate-EDTA (TBE) agarose gel prepared with 20 mM of guanidium isothiocyanate (GITC). .. For cDNA library preparation and RNA Sequencing, RNA (50 ng each) from each sample was reverse transcribed according to the instructions provided in the Ion AmpliSeq Transcriptome Human Gene Expression kit (Revision A.0, Thermo Fisher Scientific) and the generated cDNA was amplified using the Ion AmpliSeq Transcriptome Human Gene Expression core panel (Thermo Fisher Scientific).

    Article Title: Comparative genomic hybridization and transcriptome analysis with a pan-genome microarray reveal distinctions between JP2 and non-JP2 genotypes of Aggregatibacter actinomycetemcomitans
    Article Snippet: RNA Yield and Quality was assessed by Nanodrop-1000 spectrometer and Agilent bioanalyzer. .. When dry, the slides were immediately scanned using the Agilent Scanner 6000C according to the manufacturer’s recommended settings for 1-color gene expression.

    Modification:

    Article Title: Comparative genomic hybridization and transcriptome analysis with a pan-genome microarray reveal distinctions between JP2 and non-JP2 genotypes of Aggregatibacter actinomycetemcomitans
    Article Snippet: MessageAmp™ II-Bacteria kit (Life technology) was used to label the RNA(500 ng for each sample) following the protocol recommended by the manufacturer, with a slight modification in the IVT reaction in which a combination of 2.6 μL T7 CTP and 1.4 μL of cy-3 labeled CTP were used for the master mix. .. RNA Yield and Quality was assessed by Nanodrop-1000 spectrometer and Agilent bioanalyzer.

    Ligation:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: The quality of extracted RNA was assessed by checking the 260/280 and 260/230 ratios using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific) and by running samples (500 ng) on a 1.5% Tris-Borate-EDTA (TBE) agarose gel prepared with 20 mM of guanidium isothiocyanate (GITC). .. Primer sequences were partially digested followed by adaptors ligation (Ion P1 Adapter and Ion Xpress Barcode Adapter, Thermo Fisher Scientific) and purification using the Agencourt AMPure XP reagent (Beckman Coulter Inc., CA, USA).

    Infection:

    Article Title: Condition-dependent virulence of slow bee paralysis virus in Bombus terrestris: are the impacts of honeybee viruses in wild pollinators underestimated?
    Article Snippet: .. Real-time quantitative RT-PCR RNA extractions from all bee guts in the infection time course were measured on a Nanodrop 1000 spectrometer. ..

    Generated:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: The quality of extracted RNA was assessed by checking the 260/280 and 260/230 ratios using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific) and by running samples (500 ng) on a 1.5% Tris-Borate-EDTA (TBE) agarose gel prepared with 20 mM of guanidium isothiocyanate (GITC). .. For cDNA library preparation and RNA Sequencing, RNA (50 ng each) from each sample was reverse transcribed according to the instructions provided in the Ion AmpliSeq Transcriptome Human Gene Expression kit (Revision A.0, Thermo Fisher Scientific) and the generated cDNA was amplified using the Ion AmpliSeq Transcriptome Human Gene Expression core panel (Thermo Fisher Scientific).

    Protein Concentration:

    Article Title: Dissecting and Reconstructing Synergism
    Article Snippet: We measured the protein concentration of the complete cellulase system according to Bradford , employing Roti®-Quant and Roti®-Nanoquant assays (both from Carl Roth, Karlsruhe, Germany) standardized with BSA. .. The concentration of pure enzyme preparations was determined via their intrinsic UV absorbance at a wavelength of 280 nm using a Nanodrop 1000 Spectrometer (Thermo Fisher Scientific Inc., Walham, MA).

    Article Title: Dawn- and dusk-phased circadian transcription rhythms coordinate anabolic and catabolic functions in Neurospora
    Article Snippet: .. Protein concentration was determined by NanoDrop 1000 spectrometer. .. Cellulase activity was calculated by using Azo-CM-Cellulose (Megazyme, Bray, Ireland) according to the manufacturer's protocol with minor modifications.

    Polymerase Chain Reaction:

    Article Title: Pulmonary response after exposure to inhaled nickel hydroxide nanoparticles: short and long-term studies in mice
    Article Snippet: The quality and concentration was determined using a Nanodrop 1000 spectrometer (Thermo Fisher Scientific, Wilmington, DE). .. To quantify relative mRNA levels of selected genes: macrophage inflammatory protein-2 (Mip-2), Ccl2, interleukin 1-alpha (Il-1α), and tumor necrosis factor-alpha (Tnf-α), real-time polymerase chain reaction (PCR) was performed on an Applied Biosystems 7300 Real-Time PCR instrument (Applied Biosystems, Foster City, CA), under the following conditions: 10 min at 95°C followed by 40 cycles at 95°C and 60°C (15 sec and 1 min, respectively).

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: The quality of extracted RNA was assessed by checking the 260/280 and 260/230 ratios using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific) and by running samples (500 ng) on a 1.5% Tris-Borate-EDTA (TBE) agarose gel prepared with 20 mM of guanidium isothiocyanate (GITC). .. Adaptor-ligated amplicons were eluted into the amplification mix (Platinum PCR SuperMix High Fidelity and Library Amplification Primer Mix, Thermo Fisher Scientific) and amplified.

    Article Title: Dalangtan Playa (Qaidam Basin, NW China): Its microbial life and physicochemical characteristics and their astrobiological implications
    Article Snippet: Concentrations of extracted DNA were measured with a nanodrop 1000 spectrometer (Thermo Fisher Scientific Inc., USA) and Qubit 2.0 (Thermo Fisher Scientific Inc., USA). .. Inhibitors testing was designed as follows: three separate parts of prepared DNA materials (A, boiled fresh E . coli cells, B, boiled fresh E . coli cells + targeted DNA products and C, target DNA products) were set for PCR, checked by agarose gel electrophoresis, stained with ethidium bromide, and visualized by UV light.

    Article Title: Comparative genomic hybridization and transcriptome analysis with a pan-genome microarray reveal distinctions between JP2 and non-JP2 genotypes of Aggregatibacter actinomycetemcomitans
    Article Snippet: The resultant RNAs were checked for DNA contamination by PCR using 16S rRNA primers. .. RNA Yield and Quality was assessed by Nanodrop-1000 spectrometer and Agilent bioanalyzer.

    Article Title: Altered Transcription of Murine Genes Induced in the Small Bowel by Administration of Probiotic Strain Lactobacillus rhamnosus HN001
    Article Snippet: .. Products were cleaned using Qiagen PCR cleanup columns (Qiagen, Hilden, Germany) and quantified using a Nanodrop 1000 spectrometer. .. Equivalent quantities of PCR product from each sample were pooled, and the pooled DNA was recleaned through the use of a Qiagen PCR cleanup column, quantified, and sent to Macrogen (Korea) for unidirectional sequencing from the reverse primer using a Roche 454 genome sequencer with Titanium chemistry.

    Sonication:

    Article Title: Dalangtan Playa (Qaidam Basin, NW China): Its microbial life and physicochemical characteristics and their astrobiological implications
    Article Snippet: Different methods were used, (i) mechanical lysis by grinding, (ii) mechanical lysis in the presence of liquid nitrogen, (iii) mechanical lysis followed by sonication in the presence of PBS and ethanol (1:1), (iv) filtering was introduced after sonication, both the membranes mounted with sediment samples and supernatant were subjected for subsequent DNA extraction steps, (v) mechanical lysis followed by phenol extraction as described in [ ], (vi) PowerMax and PowerSoil (QIAGEN, Inc. USA) commercial extraction kits. .. Concentrations of extracted DNA were measured with a nanodrop 1000 spectrometer (Thermo Fisher Scientific Inc., USA) and Qubit 2.0 (Thermo Fisher Scientific Inc., USA).

    Immunofluorescence:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: Paragraph title: Immunofluorescence of labelled ESPs and confocal microscopy ... The amounts of labelled proteins were quantified using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific).

    DNA Extraction:

    Article Title: Dalangtan Playa (Qaidam Basin, NW China): Its microbial life and physicochemical characteristics and their astrobiological implications
    Article Snippet: Paragraph title: DNA extraction ... Concentrations of extracted DNA were measured with a nanodrop 1000 spectrometer (Thermo Fisher Scientific Inc., USA) and Qubit 2.0 (Thermo Fisher Scientific Inc., USA).

    RNA Sequencing Assay:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: Paragraph title: RNA extraction, library preparation and RNA sequencing ... The quality of extracted RNA was assessed by checking the 260/280 and 260/230 ratios using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific) and by running samples (500 ng) on a 1.5% Tris-Borate-EDTA (TBE) agarose gel prepared with 20 mM of guanidium isothiocyanate (GITC).

    Methylation:

    Article Title: Associations of LEP, CRH, ICAM-1, and LINE-1 methylation, measured in saliva, with waist circumference, body mass index, and percent body fat in mid-childhood
    Article Snippet: The concentration of eluted DNA was measured using a NanoDrop 1000 spectrometer. .. Sequencing was conducted using Pyromark Q24, and percent methylation was computed using the Pyromark Q24 Software v.2 in CpG mode.

    Isolation:

    Article Title: MicroRNA-150 Is a Potential Biomarker of HIV/AIDS Disease Progression and Therapy
    Article Snippet: RNA preparation from plasma Total RNA was isolated from plasma using the miRNeasy kit (Qiagen, Germany) with few minor modifications. .. The immobilized RNA was eluted in 50 µl of RNAse-free water and was quantified using a NanoDrop 1000 spectrometer (Thermo Scientific, Wimington, DE, USA).

    Size-exclusion Chromatography:

    Article Title: Pulmonary response after exposure to inhaled nickel hydroxide nanoparticles: short and long-term studies in mice
    Article Snippet: The quality and concentration was determined using a Nanodrop 1000 spectrometer (Thermo Fisher Scientific, Wilmington, DE). .. To quantify relative mRNA levels of selected genes: macrophage inflammatory protein-2 (Mip-2), Ccl2, interleukin 1-alpha (Il-1α), and tumor necrosis factor-alpha (Tnf-α), real-time polymerase chain reaction (PCR) was performed on an Applied Biosystems 7300 Real-Time PCR instrument (Applied Biosystems, Foster City, CA), under the following conditions: 10 min at 95°C followed by 40 cycles at 95°C and 60°C (15 sec and 1 min, respectively).

    Microscopy:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: The amounts of labelled proteins were quantified using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific). .. The fluorescent label on IECs was visualised using a Zeiss LSM700 confocal microscope (Zeiss, Oberkochen, Germany) at the BioVis Core Facility, SciLifeLab, Uppsala, Sweden.

    Purification:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: The quality of extracted RNA was assessed by checking the 260/280 and 260/230 ratios using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific) and by running samples (500 ng) on a 1.5% Tris-Borate-EDTA (TBE) agarose gel prepared with 20 mM of guanidium isothiocyanate (GITC). .. Primer sequences were partially digested followed by adaptors ligation (Ion P1 Adapter and Ion Xpress Barcode Adapter, Thermo Fisher Scientific) and purification using the Agencourt AMPure XP reagent (Beckman Coulter Inc., CA, USA).

    Article Title: Associations of LEP, CRH, ICAM-1, and LINE-1 methylation, measured in saliva, with waist circumference, body mass index, and percent body fat in mid-childhood
    Article Snippet: Mixtures were incubated in a thermocycler with 16 thermal cycles at 95 °C for 30 s and 50 °C for 1 h. Bisulfite-converted DNA samples were loaded onto 96-column plates provided in the kit for desulfonation and purification. .. The concentration of eluted DNA was measured using a NanoDrop 1000 spectrometer.

    Sequencing:

    Article Title: Transcriptome-facilitated development of SNPs for the Sonoran Desert rock fig, Ficus petiolaris (Moraceae) 1
    Article Snippet: Extracted RNA was quantified using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific Inc., Waltham, Massachusetts, USA) and then submitted to the Iowa State University (ISU) DNA Facility where it was quantified a second time using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, California, USA). .. A cDNA library was prepared from the mRNA templates using the Illumina TruSeq RNA Sample Preparation Kit V2 (Illumina, San Diego, California, USA), with library construction verified using the Agilent DNA 7500 Kit (Agilent Technologies), before transcriptome sequencing at the ISU DNA Facility on an Illumina MiSeq (Illumina) with 250-cycle paired-end reads.

    Article Title: Associations of LEP, CRH, ICAM-1, and LINE-1 methylation, measured in saliva, with waist circumference, body mass index, and percent body fat in mid-childhood
    Article Snippet: The concentration of eluted DNA was measured using a NanoDrop 1000 spectrometer. .. Sequencing was conducted using Pyromark Q24, and percent methylation was computed using the Pyromark Q24 Software v.2 in CpG mode.

    Article Title: Altered Transcription of Murine Genes Induced in the Small Bowel by Administration of Probiotic Strain Lactobacillus rhamnosus HN001
    Article Snippet: The secondary PCR was carried out for 30 cycles using the 8fAll primer with 454 sequencing Lib-A adapter sequence A (5′-CGTATCGCCTCCCTCGCGCCATCAGGRGTTYGATYMTGGCTCAG-3′) and the HDA2 primer with 454 sequencing Lib-A adapter sequence B plus a 10-base barcode (shown as N′s) (5′-CTATGCGCCTTGCCAGCCCGCTCAGNNNNNNNNNNGTATTACCGCGGCTGCTGGCAC-3′) using conditions identical to those of the primary PCR. .. Products were cleaned using Qiagen PCR cleanup columns (Qiagen, Hilden, Germany) and quantified using a Nanodrop 1000 spectrometer.

    Article Title: Metabolic reprogramming during neuronal differentiation
    Article Snippet: DNA concentrations were measured using a Nanodrop 1000 spectrometer (Thermo Scientific). .. The relative amount of mtDNA was valued by qRT-PCR performed in an ABI PRISM 7000 Sequence Detection System (Applied Biosystem) with SYBR green ready mix (Applied Biosystem) with the following primers: SDHA Fwd 5′-AGACCTTGAATGAGGCTGACTGTG-3′, SDHA Rev 5′-GAGACAAAGTCTGGCGCAACTC-3′, ND5 Fwd 5′-TGATGGTACGGACGAACAGACG-3′, and ND5 Rev 5′-CTGATGGTAGTCATGGGTGGAG-3′

    Blocking Assay:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: The amounts of labelled proteins were quantified using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific). .. Slides were washed, fixed (4% paraformaldehyde in PBS) and mounted (Vectashield anti-fade reagent with DAPI, Vector Laboratories, CA, USA), excluding the blocking step.

    Confocal Microscopy:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: Paragraph title: Immunofluorescence of labelled ESPs and confocal microscopy ... The amounts of labelled proteins were quantified using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific).

    Lysis:

    Article Title: Dalangtan Playa (Qaidam Basin, NW China): Its microbial life and physicochemical characteristics and their astrobiological implications
    Article Snippet: Different methods were used, (i) mechanical lysis by grinding, (ii) mechanical lysis in the presence of liquid nitrogen, (iii) mechanical lysis followed by sonication in the presence of PBS and ethanol (1:1), (iv) filtering was introduced after sonication, both the membranes mounted with sediment samples and supernatant were subjected for subsequent DNA extraction steps, (v) mechanical lysis followed by phenol extraction as described in [ ], (vi) PowerMax and PowerSoil (QIAGEN, Inc. USA) commercial extraction kits. .. Concentrations of extracted DNA were measured with a nanodrop 1000 spectrometer (Thermo Fisher Scientific Inc., USA) and Qubit 2.0 (Thermo Fisher Scientific Inc., USA).

    cDNA Library Assay:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: The quality of extracted RNA was assessed by checking the 260/280 and 260/230 ratios using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific) and by running samples (500 ng) on a 1.5% Tris-Borate-EDTA (TBE) agarose gel prepared with 20 mM of guanidium isothiocyanate (GITC). .. For cDNA library preparation and RNA Sequencing, RNA (50 ng each) from each sample was reverse transcribed according to the instructions provided in the Ion AmpliSeq Transcriptome Human Gene Expression kit (Revision A.0, Thermo Fisher Scientific) and the generated cDNA was amplified using the Ion AmpliSeq Transcriptome Human Gene Expression core panel (Thermo Fisher Scientific).

    Article Title: Transcriptome-facilitated development of SNPs for the Sonoran Desert rock fig, Ficus petiolaris (Moraceae) 1
    Article Snippet: Extracted RNA was quantified using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific Inc., Waltham, Massachusetts, USA) and then submitted to the Iowa State University (ISU) DNA Facility where it was quantified a second time using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, California, USA). .. A cDNA library was prepared from the mRNA templates using the Illumina TruSeq RNA Sample Preparation Kit V2 (Illumina, San Diego, California, USA), with library construction verified using the Agilent DNA 7500 Kit (Agilent Technologies), before transcriptome sequencing at the ISU DNA Facility on an Illumina MiSeq (Illumina) with 250-cycle paired-end reads.

    Agarose Gel Electrophoresis:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: .. The quality of extracted RNA was assessed by checking the 260/280 and 260/230 ratios using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific) and by running samples (500 ng) on a 1.5% Tris-Borate-EDTA (TBE) agarose gel prepared with 20 mM of guanidium isothiocyanate (GITC). .. For cDNA library preparation and RNA Sequencing, RNA (50 ng each) from each sample was reverse transcribed according to the instructions provided in the Ion AmpliSeq Transcriptome Human Gene Expression kit (Revision A.0, Thermo Fisher Scientific) and the generated cDNA was amplified using the Ion AmpliSeq Transcriptome Human Gene Expression core panel (Thermo Fisher Scientific).

    Article Title: Dalangtan Playa (Qaidam Basin, NW China): Its microbial life and physicochemical characteristics and their astrobiological implications
    Article Snippet: Concentrations of extracted DNA were measured with a nanodrop 1000 spectrometer (Thermo Fisher Scientific Inc., USA) and Qubit 2.0 (Thermo Fisher Scientific Inc., USA). .. Inhibitors testing was designed as follows: three separate parts of prepared DNA materials (A, boiled fresh E . coli cells, B, boiled fresh E . coli cells + targeted DNA products and C, target DNA products) were set for PCR, checked by agarose gel electrophoresis, stained with ethidium bromide, and visualized by UV light.

    Mouse Assay:

    Article Title: Altered Transcription of Murine Genes Induced in the Small Bowel by Administration of Probiotic Strain Lactobacillus rhamnosus HN001
    Article Snippet: Paragraph title: Pyrosequencing analysis of small-bowel microbiota of infant mice. ... Products were cleaned using Qiagen PCR cleanup columns (Qiagen, Hilden, Germany) and quantified using a Nanodrop 1000 spectrometer.

    Plasmid Preparation:

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: The amounts of labelled proteins were quantified using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific). .. Slides were washed, fixed (4% paraformaldehyde in PBS) and mounted (Vectashield anti-fade reagent with DAPI, Vector Laboratories, CA, USA), excluding the blocking step.

    Software:

    Article Title: Associations of LEP, CRH, ICAM-1, and LINE-1 methylation, measured in saliva, with waist circumference, body mass index, and percent body fat in mid-childhood
    Article Snippet: The concentration of eluted DNA was measured using a NanoDrop 1000 spectrometer. .. Sequencing was conducted using Pyromark Q24, and percent methylation was computed using the Pyromark Q24 Software v.2 in CpG mode.

    Article Title: Comparative genomic hybridization and transcriptome analysis with a pan-genome microarray reveal distinctions between JP2 and non-JP2 genotypes of Aggregatibacter actinomycetemcomitans
    Article Snippet: RNA Yield and Quality was assessed by Nanodrop-1000 spectrometer and Agilent bioanalyzer. .. The data was then Feature-extracted using Agilent’s Feature Extractor software v10.5 running the extraction protocol GE1_105_Dec08.

    SYBR Green Assay:

    Article Title: Metabolic reprogramming during neuronal differentiation
    Article Snippet: DNA concentrations were measured using a Nanodrop 1000 spectrometer (Thermo Scientific). .. The relative amount of mtDNA was valued by qRT-PCR performed in an ABI PRISM 7000 Sequence Detection System (Applied Biosystem) with SYBR green ready mix (Applied Biosystem) with the following primers: SDHA Fwd 5′-AGACCTTGAATGAGGCTGACTGTG-3′, SDHA Rev 5′-GAGACAAAGTCTGGCGCAACTC-3′, ND5 Fwd 5′-TGATGGTACGGACGAACAGACG-3′, and ND5 Rev 5′-CTGATGGTAGTCATGGGTGGAG-3′

    RNA Extraction:

    Article Title: Roles of MAPK Pathway Activation During Cytokine Induction in BEAS-2B Cells Exposed to Fine World Trade Center (WTC) Dust
    Article Snippet: Paragraph title: RNA extraction ... The pellet was then solubilized in RNase-free H2 O, heated at 50°C for 10 min, and the concentration of RNA present determined by measuring absorbance at 260 nm in a NanoDrop 1000 spectrometer (Thermo Scientific, Rockford, IL).

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: Paragraph title: RNA extraction, library preparation and RNA sequencing ... The quality of extracted RNA was assessed by checking the 260/280 and 260/230 ratios using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific) and by running samples (500 ng) on a 1.5% Tris-Borate-EDTA (TBE) agarose gel prepared with 20 mM of guanidium isothiocyanate (GITC).

    Sample Prep:

    Article Title: Transcriptome-facilitated development of SNPs for the Sonoran Desert rock fig, Ficus petiolaris (Moraceae) 1
    Article Snippet: Extracted RNA was quantified using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific Inc., Waltham, Massachusetts, USA) and then submitted to the Iowa State University (ISU) DNA Facility where it was quantified a second time using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, California, USA). .. A cDNA library was prepared from the mRNA templates using the Illumina TruSeq RNA Sample Preparation Kit V2 (Illumina, San Diego, California, USA), with library construction verified using the Agilent DNA 7500 Kit (Agilent Technologies), before transcriptome sequencing at the ISU DNA Facility on an Illumina MiSeq (Illumina) with 250-cycle paired-end reads.

    DNA Methylation Assay:

    Article Title: Associations of LEP, CRH, ICAM-1, and LINE-1 methylation, measured in saliva, with waist circumference, body mass index, and percent body fat in mid-childhood
    Article Snippet: DNA bisulfite conversion was accomplished using EZ DNA Methylation Kits (Zymo Research) by following the manufacturer’s manual. .. The concentration of eluted DNA was measured using a NanoDrop 1000 spectrometer.

    Concentration Assay:

    Article Title: Pulmonary response after exposure to inhaled nickel hydroxide nanoparticles: short and long-term studies in mice
    Article Snippet: .. The quality and concentration was determined using a Nanodrop 1000 spectrometer (Thermo Fisher Scientific, Wilmington, DE). .. Using High Capacity cDNA Reverse Transcription Kits (Applied Biosystems, Foster City, CA) and following the provided manufacturer’s protocol, total RNA was reverse transcribed into cDNA.

    Article Title: Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Article Snippet: Immunofluorescence of labelled ESPs and confocal microscopy For ESPs labelling with Alexa Fluor 488, proteins collected from axenic culture were washed (3x) with cold PBS during sample concentration to remove free amino acids and antioxidants that might interfere with protein labelling (recommended in the kit). .. The amounts of labelled proteins were quantified using a NanoDrop 1000 Spectrometer (Thermo Fisher Scientific).

    Article Title: Roles of MAPK Pathway Activation During Cytokine Induction in BEAS-2B Cells Exposed to Fine World Trade Center (WTC) Dust
    Article Snippet: .. The pellet was then solubilized in RNase-free H2 O, heated at 50°C for 10 min, and the concentration of RNA present determined by measuring absorbance at 260 nm in a NanoDrop 1000 spectrometer (Thermo Scientific, Rockford, IL). .. cDNA was synthesized using the SuperScript III first-strand synthesis supermix for qRT-PCR (Invitrogen).

    Article Title: Dissecting and Reconstructing Synergism
    Article Snippet: .. The concentration of pure enzyme preparations was determined via their intrinsic UV absorbance at a wavelength of 280 nm using a Nanodrop 1000 Spectrometer (Thermo Fisher Scientific Inc., Walham, MA). ..

    Article Title: Associations of LEP, CRH, ICAM-1, and LINE-1 methylation, measured in saliva, with waist circumference, body mass index, and percent body fat in mid-childhood
    Article Snippet: .. The concentration of eluted DNA was measured using a NanoDrop 1000 spectrometer. .. DNA methylation was quantitated using bisulfite-PCR and pyrosequencing using the following kits from Qiagen: ICAM1_02 for ICAM-1 , CRH_01 for CRH , LEP_01 for LEP , and NOS3_02 for LINE-1.

    Article Title: Characterization of RNA isolated from eighteen different human tissues: results from a rapid human autopsy program
    Article Snippet: .. The concentration and purity of each RNA sample was assessed using a Nanodrop 1000 spectrometer (Nanodrop Inc—Thermo Fisher Scientific, Wilmington, DE). .. The RNA yield from each sample was calculated as ng/mg of tissue (wet weight).

    Staining:

    Article Title: Dalangtan Playa (Qaidam Basin, NW China): Its microbial life and physicochemical characteristics and their astrobiological implications
    Article Snippet: Concentrations of extracted DNA were measured with a nanodrop 1000 spectrometer (Thermo Fisher Scientific Inc., USA) and Qubit 2.0 (Thermo Fisher Scientific Inc., USA). .. Inhibitors testing was designed as follows: three separate parts of prepared DNA materials (A, boiled fresh E . coli cells, B, boiled fresh E . coli cells + targeted DNA products and C, target DNA products) were set for PCR, checked by agarose gel electrophoresis, stained with ethidium bromide, and visualized by UV light.

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  • 99
    Thermo Fisher nanodrop nd 1000 spectrometer
    Nanodrop Nd 1000 Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodrop nd 1000 spectrometer/product/Thermo Fisher
    Average 99 stars, based on 59 article reviews
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    nanodrop nd 1000 spectrometer - by Bioz Stars, 2020-03
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