Structured Review

PEQLAB gold nanorod characterization
( A ) A gold <t>nanorod</t> (AuNR) coated with an adsorbed CTAB (Molecule 1) bilayer is functionalized with MUTAB (Molecule 2) to create a monolayer of cationic ligands covalently bound to the nanorod surface via gold-thiol bonds (top row). Alternatively, MUTAB was complemented with a rhodamine-PEG-thiol derivative (RH-PEG-SH, n = 77, Molecule 3) as a fluorescent label. Negative-stained TEM images of ( B ) CTAB-coated and ( C ) MUTAB-functionalized AuNRs.
Gold Nanorod Characterization, supplied by PEQLAB, used in various techniques. Bioz Stars score: 84/100, based on 402 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gold nanorod characterization/product/PEQLAB
Average 84 stars, based on 402 article reviews
Price from $9.99 to $1999.99
gold nanorod characterization - by Bioz Stars, 2020-08
84/100 stars

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1) Product Images from "Polycationic gold nanorods as multipurpose in vitro microtubule markers"

Article Title: Polycationic gold nanorods as multipurpose in vitro microtubule markers

Journal: bioRxiv

doi: 10.1101/2020.04.25.061127

( A ) A gold nanorod (AuNR) coated with an adsorbed CTAB (Molecule 1) bilayer is functionalized with MUTAB (Molecule 2) to create a monolayer of cationic ligands covalently bound to the nanorod surface via gold-thiol bonds (top row). Alternatively, MUTAB was complemented with a rhodamine-PEG-thiol derivative (RH-PEG-SH, n = 77, Molecule 3) as a fluorescent label. Negative-stained TEM images of ( B ) CTAB-coated and ( C ) MUTAB-functionalized AuNRs.
Figure Legend Snippet: ( A ) A gold nanorod (AuNR) coated with an adsorbed CTAB (Molecule 1) bilayer is functionalized with MUTAB (Molecule 2) to create a monolayer of cationic ligands covalently bound to the nanorod surface via gold-thiol bonds (top row). Alternatively, MUTAB was complemented with a rhodamine-PEG-thiol derivative (RH-PEG-SH, n = 77, Molecule 3) as a fluorescent label. Negative-stained TEM images of ( B ) CTAB-coated and ( C ) MUTAB-functionalized AuNRs.

Techniques Used: Staining, Transmission Electron Microscopy

2) Product Images from "Polycationic gold nanorods as multipurpose in vitro microtubule markers"

Article Title: Polycationic gold nanorods as multipurpose in vitro microtubule markers

Journal: bioRxiv

doi: 10.1101/2020.04.25.061127

( A ) AuNRs for plasmonic excitation and photoluminescence emission (magenta). AuNRs were only coated with MUTAB (blue, positively charged) interacting with a microtubule (MT, red, negatively charged) attached to an APTES (dark cyan, positively charged) coated surface (gray). ( B ) IRM, ( C ) TIRF, and ( D ) IRM-pseudocolor–TIRF overlay image of AuNRs bound to a single microtubule (green/cyan indicating different microtubule-surface distances, see Materials and Methods for details on the pseudocolor overlay). Note that no fluorophores were present and that AuNR markers did not blink or bleach.
Figure Legend Snippet: ( A ) AuNRs for plasmonic excitation and photoluminescence emission (magenta). AuNRs were only coated with MUTAB (blue, positively charged) interacting with a microtubule (MT, red, negatively charged) attached to an APTES (dark cyan, positively charged) coated surface (gray). ( B ) IRM, ( C ) TIRF, and ( D ) IRM-pseudocolor–TIRF overlay image of AuNRs bound to a single microtubule (green/cyan indicating different microtubule-surface distances, see Materials and Methods for details on the pseudocolor overlay). Note that no fluorophores were present and that AuNR markers did not blink or bleach.

Techniques Used:

3) Product Images from "MicroRNA Profiling during Cardiomyocyte-Specific Differentiation of Murine Embryonic Stem Cells Based on Two Different miRNA Array Platforms"

Article Title: MicroRNA Profiling during Cardiomyocyte-Specific Differentiation of Murine Embryonic Stem Cells Based on Two Different miRNA Array Platforms

Journal: PLoS ONE

doi: 10.1371/journal.pone.0025809

Embryonic marker genes are down regulated at day 12 of cardiomyocyte-specific differentiation. mRNA levels were measured at indicated timepoints using RT-qPCR. Relative gene expression of ES cell markers Klf4, Pou5F and Sox2 normalized to the expression of the reference gene GAPDH is shown. The results are expressed as fold change over day 0 values (undifferentiated ES cells). The y-axis represents log 2 fold change relative to day 0.
Figure Legend Snippet: Embryonic marker genes are down regulated at day 12 of cardiomyocyte-specific differentiation. mRNA levels were measured at indicated timepoints using RT-qPCR. Relative gene expression of ES cell markers Klf4, Pou5F and Sox2 normalized to the expression of the reference gene GAPDH is shown. The results are expressed as fold change over day 0 values (undifferentiated ES cells). The y-axis represents log 2 fold change relative to day 0.

Techniques Used: Marker, Quantitative RT-PCR, Expressing

4) Product Images from "Polycationic gold nanorods as multipurpose in vitro microtubule markers"

Article Title: Polycationic gold nanorods as multipurpose in vitro microtubule markers

Journal: bioRxiv

doi: 10.1101/2020.04.25.061127

( A ) AuNRs for plasmonic excitation and photoluminescence emission (magenta). AuNRs were only coated with MUTAB (blue, positively charged) interacting with a microtubule (MT, red, negatively charged) attached to an APTES (dark cyan, positively charged) coated surface (gray). ( B ) IRM, ( C ) TIRF, and ( D ) IRM-pseudocolor–TIRF overlay image of AuNRs bound to a single microtubule (green/cyan indicating different microtubule-surface distances, see Materials and Methods for details on the pseudocolor overlay). Note that no fluorophores were present and that AuNR markers did not blink or bleach.
Figure Legend Snippet: ( A ) AuNRs for plasmonic excitation and photoluminescence emission (magenta). AuNRs were only coated with MUTAB (blue, positively charged) interacting with a microtubule (MT, red, negatively charged) attached to an APTES (dark cyan, positively charged) coated surface (gray). ( B ) IRM, ( C ) TIRF, and ( D ) IRM-pseudocolor–TIRF overlay image of AuNRs bound to a single microtubule (green/cyan indicating different microtubule-surface distances, see Materials and Methods for details on the pseudocolor overlay). Note that no fluorophores were present and that AuNR markers did not blink or bleach.

Techniques Used:

Related Articles

Real-time Polymerase Chain Reaction:

Article Title: LincRNA H19 protects from dietary obesity by constraining expression of monoallelic genes in brown fat
Article Snippet: .. mRNA isolation and quantitative RT-PCR (qPCR) analysis Total RNA was isolated from primary adipocytes and tissues using peqGOLD TriFast (PEQLAB Biotechnologie). mRNA was reverse transcribed into complementary DNA using EuroScript reverse transcriptase (Eurogentec). .. Abundances of mRNAs/lncRNAs were quantified by TaqMan Assay on Demand Kits (Applied Biosystems) according to the manufacturer’s protocol if not indicated otherwise.

Article Title: Mitochondrial Ca2+ Uptake 1 (MICU1) and Mitochondrial Ca2+ Uniporter (MCU) Contribute to Metabolism-Secretion Coupling in Clonal Pancreatic β-Cells *
Article Snippet: .. mRNA Isolation and Real Time PCR Total RNA was isolated using the PEQLAB total RNA isolation kit (PEQLAB Biotechnologie GmbH, Erlangen, Germany), and reverse transcription was performed in a thermal cycler (PEQLAB Biotechnologie GmbH) using a cDNA synthesis kit (Applied Biosystems). .. Expression of GAPDH, MICU1, MCU, LETM1, and UCP2 in INS-1 832/13 cells was examined by PCR and agarose gel electrophoresis.

Isolation:

Article Title: LincRNA H19 protects from dietary obesity by constraining expression of monoallelic genes in brown fat
Article Snippet: .. mRNA isolation and quantitative RT-PCR (qPCR) analysis Total RNA was isolated from primary adipocytes and tissues using peqGOLD TriFast (PEQLAB Biotechnologie). mRNA was reverse transcribed into complementary DNA using EuroScript reverse transcriptase (Eurogentec). .. Abundances of mRNAs/lncRNAs were quantified by TaqMan Assay on Demand Kits (Applied Biosystems) according to the manufacturer’s protocol if not indicated otherwise.

Article Title: Inflammation-induced loss of Pdcd4 is mediated by phosphorylation-dependent degradation
Article Snippet: .. Twenty-four hours after reseeding, U937 cells were harvested for mRNA isolation. mRNA was isolated using PeqGold RNAPure (peqLab, Erlangen, Germany) according to the manufacturer’s manual. .. RNA was transcribed using the Maxima First Strand cDNA synthesis kit from Fermentas (St. Leon-Rot, Germany) and expression of individual mRNAs was analyzed using real-time PCR with Absolute qPCR SYBR Green Fluorescent mix (Abgene, Surrey, UK).

Article Title: Mitochondrial Ca2+ Uptake 1 (MICU1) and Mitochondrial Ca2+ Uniporter (MCU) Contribute to Metabolism-Secretion Coupling in Clonal Pancreatic β-Cells *
Article Snippet: .. mRNA Isolation and Real Time PCR Total RNA was isolated using the PEQLAB total RNA isolation kit (PEQLAB Biotechnologie GmbH, Erlangen, Germany), and reverse transcription was performed in a thermal cycler (PEQLAB Biotechnologie GmbH) using a cDNA synthesis kit (Applied Biosystems). .. Expression of GAPDH, MICU1, MCU, LETM1, and UCP2 in INS-1 832/13 cells was examined by PCR and agarose gel electrophoresis.

other:

Article Title: Association of Human FOS Promoter Variants with the Occurrence of Knee-Osteoarthritis in a Case Control Association Study
Article Snippet: DNA concentrations were determined using the Nanodrop ND-1000 system (PeqLab, Erlangen, Germany).

Article Title: Polycationic gold nanorods as multipurpose in vitro microtubule markers
Article Snippet: Gold nanorod characterization To determine the longitudinal plasmonic resonance, size, and surface potential of AuNRs, we used a Peqlab (Erlangen, Germany) Nanodrop ND-1000 spectrofluorometer (UV/Vis function) and a Malvern (Worcestershire, United Kingdom) Zetasizer Nano ZS for dynamic light scattering and zeta potential measurements.

Quantitative RT-PCR:

Article Title: LincRNA H19 protects from dietary obesity by constraining expression of monoallelic genes in brown fat
Article Snippet: .. mRNA isolation and quantitative RT-PCR (qPCR) analysis Total RNA was isolated from primary adipocytes and tissues using peqGOLD TriFast (PEQLAB Biotechnologie). mRNA was reverse transcribed into complementary DNA using EuroScript reverse transcriptase (Eurogentec). .. Abundances of mRNAs/lncRNAs were quantified by TaqMan Assay on Demand Kits (Applied Biosystems) according to the manufacturer’s protocol if not indicated otherwise.

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