Structured Review

Waters Corporation nanoacquity nanohplc system
Nanoacquity Nanohplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nanoacquity nanohplc system/product/Waters Corporation
Average 88 stars, based on 2 article reviews
Price from $9.99 to $1999.99
nanoacquity nanohplc system - by Bioz Stars, 2020-09
88/100 stars

Related Products / Commonly Used Together

ion-trap ltq - orbitrap elite hybrid
nanohplc-ms/ms analysis

Images

Related Articles

other:

Article Title: Ubiquitination independent of E1 and E2 enzymes by bacterial effectors
Article Snippet: Resulting peptides were analyzed in a NanoAcquity nanoHPLC system (Waters) by loading peptides into a trap column (5 cm × 150 μm i.d. column packed in-lab with 5-μm Jupiter C18 stationary phase) and separated in a 40 cm × 75 μm i.d. column packed in-lab with 3-μm Jupiter C18 stationary phase.

Mass Spectrometry:

Article Title: The Chikungunya Virus Capsid Protein Contains Linear B Cell Epitopes in the N- and C-Terminal Regions that are Dependent on an Intact C-Terminus for Antibody Recognition
Article Snippet: .. Acidified digests were subjected to NanoHPLC-MS/MS analysis using a nanoAcquity nanoHPLC system (Waters, Milford, MA, USA) interfaced with a linear ion-trap (LTQ)-Orbitrap Elite hybrid mass spectrometer (Thermo Fischer Scientific, Bremen, Germany). .. Digests were loaded onto a 5 μm Symmetry 180 μm × 20 mm C18 trap column (Waters) at 15 μL/min in 98% solvent A (0.1% (v/v ) aqueous formic acid) and 2% solvent B (0.1% (v/v ) formic acid in 100% acetonitrile) for 3 min at 22 °C then switched in-line with a pre-equilibrated analytical column (BEH130 C18 1.7 μm, 75 μm × 200 mm, Waters) at a flow rate of 0.3 μL/min and 98% solvent A, 2% solvent B. Peptides were separated at 35 °C using a sequence of linear gradients: starting from 5% B over 1 min, to 40% B over 29 min, and finally, to 95% B over 4 min, before holding the column at 95% B for a further 4 min. Eluates from the analytical column were then introduced into the LTQ-Orbitrap Elite throughout the entire run via a Nanospray Flex Ion Source (Thermo Fisher Scientific) containing a 10 μm P200P coated silica emitter (New Objective).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Waters Corporation nanoacquity nanouplc system
    Protein adsorption onto different Ti-surfaces. The Ti-discs were incubated in cell medium containing 10% bovine serum. The adsorbed proteins were extracted from titanium discs and the samples then prepared and digested with trypsin. Then, identified by LC/MS in a <t>nanoacquity</t> <t>nanoUPLC</t> system coupled toa Synapt G1 Q-TOF mass spectrometer. A ) The total protein adsorption at per square centimeter; B ) Relative amount of vitronectin: in this regard, it was possible to verify that MBAA's surface properties provided a better adsorption of vitronectin when it was compared with others; C ) Graphical presentation of focal adhesion pathway obtained from KEGG database. Considering the role of vitronectin on osteoblast adhesion, it is possible that the impact of these nanosurfaces on cell adhesion may be mediated by protein adsorption.
    Nanoacquity Nanouplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanoacquity nanouplc system/product/Waters Corporation
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    nanoacquity nanouplc system - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Protein adsorption onto different Ti-surfaces. The Ti-discs were incubated in cell medium containing 10% bovine serum. The adsorbed proteins were extracted from titanium discs and the samples then prepared and digested with trypsin. Then, identified by LC/MS in a nanoacquity nanoUPLC system coupled toa Synapt G1 Q-TOF mass spectrometer. A ) The total protein adsorption at per square centimeter; B ) Relative amount of vitronectin: in this regard, it was possible to verify that MBAA's surface properties provided a better adsorption of vitronectin when it was compared with others; C ) Graphical presentation of focal adhesion pathway obtained from KEGG database. Considering the role of vitronectin on osteoblast adhesion, it is possible that the impact of these nanosurfaces on cell adhesion may be mediated by protein adsorption.

    Journal: PLoS ONE

    Article Title: Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations

    doi: 10.1371/journal.pone.0095662

    Figure Lengend Snippet: Protein adsorption onto different Ti-surfaces. The Ti-discs were incubated in cell medium containing 10% bovine serum. The adsorbed proteins were extracted from titanium discs and the samples then prepared and digested with trypsin. Then, identified by LC/MS in a nanoacquity nanoUPLC system coupled toa Synapt G1 Q-TOF mass spectrometer. A ) The total protein adsorption at per square centimeter; B ) Relative amount of vitronectin: in this regard, it was possible to verify that MBAA's surface properties provided a better adsorption of vitronectin when it was compared with others; C ) Graphical presentation of focal adhesion pathway obtained from KEGG database. Considering the role of vitronectin on osteoblast adhesion, it is possible that the impact of these nanosurfaces on cell adhesion may be mediated by protein adsorption.

    Article Snippet: Samples were then prepared and digested with trypsin and identified by LC/MS in a nanoacquity nanoUPLC system coupled to a Synapt G1 Q-TOF mass spectrometer (Waters, USA).

    Techniques: Adsorption, Incubation, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry