nano electrospray ionization nsi easy spray source  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nano electrospray ionization nsi easy spray source
    Nano Electrospray Ionization Nsi Easy Spray Source, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano electrospray ionization nsi easy spray source/product/Thermo Fisher
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    nano electrospray ionization nsi easy spray source - by Bioz Stars, 2020-02
    93/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
    Article Snippet: After 5 min of flow at 5 µl/min with the loading pump, the 10-port valve switched to analysis mode in which the NC pump provided a flow of 250 nL/min through the guard column. .. Total LC-MS run time was 74 min. We used a nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C.

    Article Title: Discovery of coding regions in the human genome by integrated proteogenomics analysis workflow
    Article Snippet: After 5 min of flow at 5 µl min−1 with the loading pump, the 10-port valve switched to analysis mode in which the NC pump provided a flow of 250 nL min−1 through the guard column. .. We used a nano-EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60 °C.

    Article Title: Isoelectric point-based fractionation by HiRIEF coupled to LC-MS allows for in-depth quantitative analysis of the phosphoproteome
    Article Snippet: Following 5 min of flow at 5 µl/min driven by the loading pump, the 10-port valve switched to analysis mode in which the binary high-pressure gradient pump (referred to as nano gradient, NG, pump) provided a flow of 250 nL/min through the guard desalting column. .. Total LC-MS run time was 74 min. A nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo Scientific) was used on the nano electrospray ionization (NSI) EASY-Spray source (Thermo Scientific) at 60 °C.

    Article Title: Synaptic markers of cognitive decline in neurodegenerative diseases: a proteomic approach
    Article Snippet: After 5 min of flow at 5 µl/min with the loading pump, the 10-port valve switched to analysis mode in which the NC pump provided a flow of 250 nl/min through the guard column. .. Total LC-MS run time was 74 min. We used a nano EASY-Spray™ column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano-electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C.

    Mass Spectrometry:

    Article Title: In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
    Article Snippet: LC-ESI-MS/MS Q-Exactive Online LC-MS was performed using a hybrid Q-Exactive mass spectrometer (Thermo Scientific). .. Total LC-MS run time was 74 min. We used a nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C.

    Article Title: Discovery of coding regions in the human genome by integrated proteogenomics analysis workflow
    Article Snippet: We used a nano-EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60 °C. .. Online LC-MS was performed using a hybrid Q-Exactive mass spectrometer (Thermo Scientific).

    Article Title: Isoelectric point-based fractionation by HiRIEF coupled to LC-MS allows for in-depth quantitative analysis of the phosphoproteome
    Article Snippet: Paragraph title: Liquid chromatography tandem mass spectrometry ... Total LC-MS run time was 74 min. A nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo Scientific) was used on the nano electrospray ionization (NSI) EASY-Spray source (Thermo Scientific) at 60 °C.

    Article Title: Synaptic markers of cognitive decline in neurodegenerative diseases: a proteomic approach
    Article Snippet: Total LC-MS run time was 74 min. We used a nano EASY-Spray™ column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano-electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C. .. Online LC-MS was performed using a hybrid Q-Exactive mass spectrometer (Thermo Scientific).

    Chromatography:

    Article Title: Synaptic markers of cognitive decline in neurodegenerative diseases: a proteomic approach
    Article Snippet: For each liquid chromatography–mass spectrometry (LC-MS) run of a HiRIEF fraction, the autosampler (Ultimate 3000 RSLC nanoUPLC system, Thermo Scientific Dionex) dispensed 15 µl of mobile phase A [95% water, 5% dimethylsulphoxide (DMSO), 0.1% formic acid] into the corresponding well of the microtitre plate, mixed by aspirating/dispensing 10 µl 10 times, and finally injected 7 µl into a C18 guard desalting column (Acclaim pepmap 100, 75 µm × 2 cm, nanoViper, Thermo). .. Total LC-MS run time was 74 min. We used a nano EASY-Spray™ column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano-electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C.

    Isolation:

    Article Title: In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
    Article Snippet: Total LC-MS run time was 74 min. We used a nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C. .. Precursors were isolated with a 2 m/z window.

    Article Title: Discovery of coding regions in the human genome by integrated proteogenomics analysis workflow
    Article Snippet: We used a nano-EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60 °C. .. Precursors were isolated with a 2 m/z window.

    Article Title: Isoelectric point-based fractionation by HiRIEF coupled to LC-MS allows for in-depth quantitative analysis of the phosphoproteome
    Article Snippet: Total LC-MS run time was 74 min. A nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo Scientific) was used on the nano electrospray ionization (NSI) EASY-Spray source (Thermo Scientific) at 60 °C. .. Precursor ions were isolated with a 2 m/z window.

    Article Title: Synaptic markers of cognitive decline in neurodegenerative diseases: a proteomic approach
    Article Snippet: Total LC-MS run time was 74 min. We used a nano EASY-Spray™ column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano-electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C. .. Precursors were isolated with a 2 m/z window.

    Stripping Membranes:

    Article Title: Isoelectric point-based fractionation by HiRIEF coupled to LC-MS allows for in-depth quantitative analysis of the phosphoproteome
    Article Snippet: For Standard HiRIEF, all 72 fractions from the wide-range IPG strip plate were analyzed with LC-MS (Standard HiRIEF LC-MS). .. Total LC-MS run time was 74 min. A nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo Scientific) was used on the nano electrospray ionization (NSI) EASY-Spray source (Thermo Scientific) at 60 °C.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
    Article Snippet: .. Total LC-MS run time was 74 min. We used a nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C. .. FTMS master scans with 70,000 resolution (and mass range 300–1700 m/z) were followed by data-dependent MS/MS (35 000 resolution) on the top five ions using higher energy collision dissociation (HCD) at 30–40% normalized collision energy.

    Article Title: Discovery of coding regions in the human genome by integrated proteogenomics analysis workflow
    Article Snippet: Total LC-MS run time was 74 min (89 min for normal tissues). .. We used a nano-EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60 °C.

    Article Title: Isoelectric point-based fractionation by HiRIEF coupled to LC-MS allows for in-depth quantitative analysis of the phosphoproteome
    Article Snippet: .. Total LC-MS run time was 74 min. A nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo Scientific) was used on the nano electrospray ionization (NSI) EASY-Spray source (Thermo Scientific) at 60 °C. .. Online LC-MS was performed using a hybrid Q-Exactive mass spectrometer (Thermo Scientific).

    Article Title: Synaptic markers of cognitive decline in neurodegenerative diseases: a proteomic approach
    Article Snippet: .. Total LC-MS run time was 74 min. We used a nano EASY-Spray™ column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano-electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C. .. Online LC-MS was performed using a hybrid Q-Exactive mass spectrometer (Thermo Scientific).

    Tandem Mass Spectroscopy:

    Article Title: In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
    Article Snippet: Total LC-MS run time was 74 min. We used a nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C. .. FTMS master scans with 70,000 resolution (and mass range 300–1700 m/z) were followed by data-dependent MS/MS (35 000 resolution) on the top five ions using higher energy collision dissociation (HCD) at 30–40% normalized collision energy.

    Article Title: Discovery of coding regions in the human genome by integrated proteogenomics analysis workflow
    Article Snippet: We used a nano-EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60 °C. .. FTMS master scans with 70,000 resolution (and mass range 300–1700 m/z) were followed by data-dependent MS/MS (35,000 resolution) on the top 5 ions using higher energy collision dissociation (HCD) at 30% normalized collision energy.

    Article Title: Isoelectric point-based fractionation by HiRIEF coupled to LC-MS allows for in-depth quantitative analysis of the phosphoproteome
    Article Snippet: Total LC-MS run time was 74 min. A nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo Scientific) was used on the nano electrospray ionization (NSI) EASY-Spray source (Thermo Scientific) at 60 °C. .. Fourier transform-based mass spectrometer (FTMS) master scans with a resolution of 70,000 (and mass range 300–1,700 m/z) were followed by data-dependent MS/MS (35,000 resolution) on the 5 most abundant ions using higher energy collision dissociation (HCD) at 30% normalized collision energy.

    Article Title: Synaptic markers of cognitive decline in neurodegenerative diseases: a proteomic approach
    Article Snippet: Total LC-MS run time was 74 min. We used a nano EASY-Spray™ column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano-electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C. .. FTMS master scans with 70 000 resolution (and mass range 300–1600 m/z) were followed by data-dependent MS/MS (35 000 resolution) on the top five ions using higher energy collision dissociation (HCD) at 30% normalized collision energy.

    Injection:

    Article Title: Discovery of coding regions in the human genome by integrated proteogenomics analysis workflow
    Article Snippet: LC-MS/MS analysis For each LC-MS run of a HiRIEF fraction, the auto sampler (Ultimate 3000 RSLC system, Thermo Scientific Dionex) dispensed 15 µl of mobile phase A (95% water, 5% dimethylsulfoxide (DMSO), 0.1% formic acid) into the corresponding well of the microtiter plate (96 well V-bottom, polypropylene, Greiner), mixed by aspirating/dispensing 10 µl ten times, and finally injected 7 µl into a C18 guard desalting column (Acclaim pepmap 100, 75 µm × 2 cm, nanoViper, Thermo). .. We used a nano-EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60 °C.

    Article Title: Synaptic markers of cognitive decline in neurodegenerative diseases: a proteomic approach
    Article Snippet: For each liquid chromatography–mass spectrometry (LC-MS) run of a HiRIEF fraction, the autosampler (Ultimate 3000 RSLC nanoUPLC system, Thermo Scientific Dionex) dispensed 15 µl of mobile phase A [95% water, 5% dimethylsulphoxide (DMSO), 0.1% formic acid] into the corresponding well of the microtitre plate, mixed by aspirating/dispensing 10 µl 10 times, and finally injected 7 µl into a C18 guard desalting column (Acclaim pepmap 100, 75 µm × 2 cm, nanoViper, Thermo). .. Total LC-MS run time was 74 min. We used a nano EASY-Spray™ column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano-electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C.

    Liquid Chromatography:

    Article Title: Isoelectric point-based fractionation by HiRIEF coupled to LC-MS allows for in-depth quantitative analysis of the phosphoproteome
    Article Snippet: Paragraph title: Liquid chromatography tandem mass spectrometry ... Total LC-MS run time was 74 min. A nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo Scientific) was used on the nano electrospray ionization (NSI) EASY-Spray source (Thermo Scientific) at 60 °C.

    Software:

    Article Title: In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
    Article Snippet: The curved gradient (curve six in the Chromeleon software) then proceeded from 3% mobile phase B (95% acetonitrile, 5% water, 0.1% formic acid) to 45% B in 50 min followed by wash at 99% B and re-equilibration. .. Total LC-MS run time was 74 min. We used a nano EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C.

    Article Title: Discovery of coding regions in the human genome by integrated proteogenomics analysis workflow
    Article Snippet: The slightly concave curved gradient (curve 6 in the Chromeleon software) then proceeded from 3% mobile phase B (90% acetonitrile, 5% DMSO, 5% water, 0.1% formic acid) to 45% B in 50 min (65 min for normal tissues) followed by wash at 99% B and re-equilibration. .. We used a nano-EASY-Spray column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano electrospray ionization (NSI) EASY-Spray source (Thermo) at 60 °C.

    Article Title: Synaptic markers of cognitive decline in neurodegenerative diseases: a proteomic approach
    Article Snippet: The curved gradient (curve 6 in the Chromeleon software) then proceeded from 3% mobile phase B (90% acetonitrile, 5% DMSO, 5% water, 0.1% formic acid) to 45% B in 50 min followed by wash at 99% B and re-equilibration. .. Total LC-MS run time was 74 min. We used a nano EASY-Spray™ column (pepmap RSLC, C18, 2 µm bead size, 100 Å, 75 µm internal diameter, 50 cm long, Thermo) on the nano-electrospray ionization (NSI) EASY-Spray source (Thermo) at 60°C.

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  • About
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  • Team
  • Advisors
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  • Contact
  • Bioz Stars
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  • 93
    Thermo Fisher nano electrospray ionization nsi easy spray source
    Nano Electrospray Ionization Nsi Easy Spray Source, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano electrospray ionization nsi easy spray source/product/Thermo Fisher
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    nano electrospray ionization nsi easy spray source - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

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