nano drop spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nano drop spectrometer
    Nano Drop Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano drop spectrometer/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nano drop spectrometer - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Induction of RNAi Core Machinery’s Gene Expression by Exogenous dsRNA and the Effects of Pre-exposure to dsRNA on the Gene Silencing Efficiency in the Pea Aphid (Acyrthosiphon pisum)
    Article Snippet: Paragraph title: RNA Extraction, cDNA Synthesis and Cloning ... The RNA was then quantified using a NanoDrop One microvolume UV-Vis spectrophotometer (Thermo Scientific, Wilmington, MA, United States).

    Amplification:

    Article Title: Characterizing the soil microbiome and quantifying antibiotic resistance gene dynamics in agricultural soil following swine CAFO manure application
    Article Snippet: qPCR for gene targets Quantitative Real-Time PCR amplification was performed using an Applied Biosystems StepOnePlus Real-Time PCR System (ThermoFisher, Waltham, MA). .. Plasmid copy number was calculated using the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher, Waltham, MA).

    Article Title: Altered Gut Microbiota and Compositional Changes in Firmicutes and Proteobacteria in Mexican Undernourished and Obese Children
    Article Snippet: Paragraph title: DNA Extraction and Preparation of 16S rRNA Gene Amplicon Libraries ... DNA concentrations were measured by using Nano Drop V3.8.1 spectrophotometer.

    Article Title: Differential expression of heat shock proteins and antioxidant enzymes in response to temperature, starvation, and parasitism in the Carob moth larvae, Ectomyelois ceratoniae (Lepidoptera: Pyralidae)
    Article Snippet: Preparing dsRNA Template DNA was amplified with the gene-specific primers that possessed the T7 RNA polymerase promoter sequence at the 5’ end ( 5-TAATACGACTCACTATAG-3 ) ( ). .. The concentration and quality of dsRNAs were assessed with a Nanodrop spectrophotometer.

    Synthesized:

    Article Title: Induction of RNAi Core Machinery’s Gene Expression by Exogenous dsRNA and the Effects of Pre-exposure to dsRNA on the Gene Silencing Efficiency in the Pea Aphid (Acyrthosiphon pisum)
    Article Snippet: The RNA was then quantified using a NanoDrop One microvolume UV-Vis spectrophotometer (Thermo Scientific, Wilmington, MA, United States). .. The first-strand cDNA from mRNA was synthesized using a PrimerScript RT Reagent Kit (TaKaRa, Dalian, China) following the manufacturer’s protocol.

    Quantitative RT-PCR:

    Article Title: Synthetic presentation of noncanonical Wnt5a motif promotes mechanosensing-dependent differentiation of stem cells and regeneration
    Article Snippet: Paragraph title: Gene expression analysis with RT-qPCR ... The RNA concentration was measured using a NanoDrop One spectrophotometer (NanoDrop Technologies, Waltham, Massachusetts, USA).

    SYBR Green Assay:

    Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function
    Article Snippet: .. Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system. ..

    Article Title: Characterizing the soil microbiome and quantifying antibiotic resistance gene dynamics in agricultural soil following swine CAFO manure application
    Article Snippet: Plasmid copy number was calculated using the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher, Waltham, MA). .. Each reaction was prepared using 12.5 μl of Takyon ROX SYBR serial dilutions that spanned the range of the target gene amplification from environmental MasterMix blue dTTP (Eurogentec, Fremont, CA) for SYBR green PCR and PrimeTime Gene Expression Master Mix (Integrated DNA Technologies, Coralville, IA) for TaqMan PCR.

    Microarray:

    Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function
    Article Snippet: Total RNA was sent to Exiqon for microarray analysis. .. Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system.

    Incubation:

    Article Title: The effects of in utero bisphenol A exposure on ovarian follicle numbers and steroidogenesis in the F1 and F2 generations of mice
    Article Snippet: Total RNA was extracted from the ovaries using the RNeasy Mini Kit (Qiagen, Inc., Valencia, CA) and quantified with the Nanodrop® spectrophotometer following manufacturer's instructions. .. The qPCR program protocol was based on previously published methods [ ],[ ], with minor modifications: initial incubation at 95°C for five minutes (instead of 10 minutes) and final extension at 72°C for two minutes (instead of 10 minutes).

    Article Title: Biological Effects of Functionalizing Copolymer Scaffolds with Nanodiamond Particles
    Article Snippet: The cell/scaffold constructs were incubated overnight, and then transferred to spinner flasks, a procedure described in. .. The protein and DNA level were also measured directly on a Nanodrop® Spectrophotometer as described above.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Detection of KRAS mutation via ligation-initiated LAMP reaction
    Article Snippet: Genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples was extracted by TIANamp Genomic DNA Kit according to the manufacture’s instruction, and stored at −80 °C until use, and the final concentration of genomic DNA was 0.4 ng µL−1 . .. The DNA concentration was determined by NanoDrop One Microvolume UV-vis spectrophotometer (Thermo Scientific, USA).

    Expressing:

    Article Title: Synthetic presentation of noncanonical Wnt5a motif promotes mechanosensing-dependent differentiation of stem cells and regeneration
    Article Snippet: Paragraph title: Gene expression analysis with RT-qPCR ... The RNA concentration was measured using a NanoDrop One spectrophotometer (NanoDrop Technologies, Waltham, Massachusetts, USA).

    Article Title: Characterizing the soil microbiome and quantifying antibiotic resistance gene dynamics in agricultural soil following swine CAFO manure application
    Article Snippet: Plasmid copy number was calculated using the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher, Waltham, MA). .. Each reaction was prepared using 12.5 μl of Takyon ROX SYBR serial dilutions that spanned the range of the target gene amplification from environmental MasterMix blue dTTP (Eurogentec, Fremont, CA) for SYBR green PCR and PrimeTime Gene Expression Master Mix (Integrated DNA Technologies, Coralville, IA) for TaqMan PCR.

    Article Title: Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine
    Article Snippet: Paragraph title: Gene Expression Profile ... After RNA quantification using a NanoDropTM spectrophotometer, 500 ng was processed to obtain cDNA by High Capacity cDNA Reverse Transcription (#4368814; Applied Biosystems).

    Western Blot:

    Article Title: Transcriptome analysis of Inbred Long Sleep and Inbred Short Sleep mice
    Article Snippet: .. Total RNA, from whole brains (WB, n=6, 3/strain) and striatum (ST, n=6, 3/strain) was extracted using RNeasy midi kits (Qiagen, Valencia, CA), and quantity and quality were determined using a NanoDrop™ spectrophotometer (Thermo Fisher Scientific, Wilmington, DE) and Agilent 2100 BioAnalyzer™ (Agilent Technologies, Santa Clara, CA). ..

    Transformation Assay:

    Article Title: Characterizing the soil microbiome and quantifying antibiotic resistance gene dynamics in agricultural soil following swine CAFO manure application
    Article Snippet: Plasmids were transformed into Escherichia coli One Shot TOP10 Cells (Thermofisher, Waltham, MA) using the manufacturer’s instructions and were extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega, Madison, WI) following the manufacturer’s instructions. .. Plasmid copy number was calculated using the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher, Waltham, MA).

    Sequencing:

    Article Title: Full-Length Transcriptome Survey and Expression Analysis of Parasitoid Wasp Chouioia cunea upon Exposure to 1-Dodecene
    Article Snippet: For Illumina sequencing, one-day-old female C. cunea adults were exposed to 1-Dodecene or not for 1 hour, and then immersed in RNA Later (Ambion, AM7020) and collected in RNase-free Tubes. .. The concentration and quality of RNA were determined using a Nanodrop spectrophotometer.

    Article Title: Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine
    Article Snippet: After RNA quantification using a NanoDropTM spectrophotometer, 500 ng was processed to obtain cDNA by High Capacity cDNA Reverse Transcription (#4368814; Applied Biosystems). .. An Applied Biosystems Prism 7900HT Sequence Detection System was used, with the Sequence Detector Software (SDS version 2.0; Applied Biosystems) ( ).

    Article Title: Differential expression of heat shock proteins and antioxidant enzymes in response to temperature, starvation, and parasitism in the Carob moth larvae, Ectomyelois ceratoniae (Lepidoptera: Pyralidae)
    Article Snippet: Preparing dsRNA Template DNA was amplified with the gene-specific primers that possessed the T7 RNA polymerase promoter sequence at the 5’ end ( 5-TAATACGACTCACTATAG-3 ) ( ). .. The concentration and quality of dsRNAs were assessed with a Nanodrop spectrophotometer.

    Injection:

    Article Title: Induction of RNAi Core Machinery’s Gene Expression by Exogenous dsRNA and the Effects of Pre-exposure to dsRNA on the Gene Silencing Efficiency in the Pea Aphid (Acyrthosiphon pisum)
    Article Snippet: RNA Extraction, cDNA Synthesis and Cloning To monitor mRNA levels of different target genes post-dsRNA injection, all experiments contained four replicates (each replicate was injected at the same time) with four individuals per replicate. .. The RNA was then quantified using a NanoDrop One microvolume UV-Vis spectrophotometer (Thermo Scientific, Wilmington, MA, United States).

    DNA Extraction:

    Article Title: Detection of KRAS mutation via ligation-initiated LAMP reaction
    Article Snippet: Paragraph title: Genomic DNA extraction and purification from clinical samples ... The DNA concentration was determined by NanoDrop One Microvolume UV-vis spectrophotometer (Thermo Scientific, USA).

    Article Title: Altered Gut Microbiota and Compositional Changes in Firmicutes and Proteobacteria in Mexican Undernourished and Obese Children
    Article Snippet: Paragraph title: DNA Extraction and Preparation of 16S rRNA Gene Amplicon Libraries ... DNA concentrations were measured by using Nano Drop V3.8.1 spectrophotometer.

    Nucleic Acid Electrophoresis:

    Article Title: Acquisition of fluoroquinolone resistance leads to increased biofilm formation and pathogenicity in Campylobacter jejuni
    Article Snippet: Paragraph title: Chloroquine gel electrophoresis to visualize in vivo plasmid DNA supercoiling profiles ... Plasmid isolation was carried out on 20 ml of overnight cultures using the QIAgen MiniPrep kit and DNA concentrations were normalized using a NanoDrop spectrophotometer.

    In Vivo:

    Article Title: Acquisition of fluoroquinolone resistance leads to increased biofilm formation and pathogenicity in Campylobacter jejuni
    Article Snippet: Paragraph title: Chloroquine gel electrophoresis to visualize in vivo plasmid DNA supercoiling profiles ... Plasmid isolation was carried out on 20 ml of overnight cultures using the QIAgen MiniPrep kit and DNA concentrations were normalized using a NanoDrop spectrophotometer.

    Isolation:

    Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function
    Article Snippet: The upper aqueous layer was collected and RNA was isolated using RNeasy spin columns, as per manufacturer’s protocols. .. Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system.

    Article Title: Acquisition of fluoroquinolone resistance leads to increased biofilm formation and pathogenicity in Campylobacter jejuni
    Article Snippet: .. Plasmid isolation was carried out on 20 ml of overnight cultures using the QIAgen MiniPrep kit and DNA concentrations were normalized using a NanoDrop spectrophotometer. ..

    Article Title: Mapping Semaphorins and Netrins in the Pathogenesis of Human Thoracic Aortic Aneurysms
    Article Snippet: Paragraph title: 4.2. RNA Isolation ... Concentration and quality of RNA were verified by a NanoDrop One microvolume UV-vis spectrophotometer (ND-ONEW-W, Thermofisher Scientific, Wilmington, DE, USA).

    Purification:

    Article Title: Detection of KRAS mutation via ligation-initiated LAMP reaction
    Article Snippet: Paragraph title: Genomic DNA extraction and purification from clinical samples ... The DNA concentration was determined by NanoDrop One Microvolume UV-vis spectrophotometer (Thermo Scientific, USA).

    Polymerase Chain Reaction:

    Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function
    Article Snippet: .. Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system. ..

    Article Title: Characterizing the soil microbiome and quantifying antibiotic resistance gene dynamics in agricultural soil following swine CAFO manure application
    Article Snippet: Plasmid copy number was calculated using the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher, Waltham, MA). .. Each reaction was prepared using 12.5 μl of Takyon ROX SYBR serial dilutions that spanned the range of the target gene amplification from environmental MasterMix blue dTTP (Eurogentec, Fremont, CA) for SYBR green PCR and PrimeTime Gene Expression Master Mix (Integrated DNA Technologies, Coralville, IA) for TaqMan PCR.

    Article Title: Altered Gut Microbiota and Compositional Changes in Firmicutes and Proteobacteria in Mexican Undernourished and Obese Children
    Article Snippet: DNA concentrations were measured by using Nano Drop V3.8.1 spectrophotometer. .. The V3–V4 hypervariable region of bacterial 16S rRNA gene was amplified by GeneAmp PCR system 9700 (Applied Biosystems).

    Article Title: Differential expression of heat shock proteins and antioxidant enzymes in response to temperature, starvation, and parasitism in the Carob moth larvae, Ectomyelois ceratoniae (Lepidoptera: Pyralidae)
    Article Snippet: The PCR products were used for the preparation of dsRNA using the MEGA Script RNAi kit according to the manufacturer’s instructions (Ambion, TX). .. The concentration and quality of dsRNAs were assessed with a Nanodrop spectrophotometer.

    Mouse Assay:

    Article Title: The effects of in utero bisphenol A exposure on ovarian follicle numbers and steroidogenesis in the F1 and F2 generations of mice
    Article Snippet: Ovaries from the F1 mice at three and 10 months of age and from the F2 mice at three and 12 months of age were collected and snap frozen for quantitative real-time polymerase chain reaction (qPCR) analysis of steroidogenic enzymes. .. Total RNA was extracted from the ovaries using the RNeasy Mini Kit (Qiagen, Inc., Valencia, CA) and quantified with the Nanodrop® spectrophotometer following manufacturer's instructions.

    Article Title: Transcriptome analysis of Inbred Long Sleep and Inbred Short Sleep mice
    Article Snippet: On post-natal day 60, twelve ethanol-naïve adult male mice (n=6/strain) were sacrificed by cervical dislocation and whole brains were removed. .. Total RNA, from whole brains (WB, n=6, 3/strain) and striatum (ST, n=6, 3/strain) was extracted using RNeasy midi kits (Qiagen, Valencia, CA), and quantity and quality were determined using a NanoDrop™ spectrophotometer (Thermo Fisher Scientific, Wilmington, DE) and Agilent 2100 BioAnalyzer™ (Agilent Technologies, Santa Clara, CA).

    Plasmid Preparation:

    Article Title: Characterizing the soil microbiome and quantifying antibiotic resistance gene dynamics in agricultural soil following swine CAFO manure application
    Article Snippet: .. Plasmid copy number was calculated using the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher, Waltham, MA). .. Each reaction was prepared using 12.5 μl of Takyon ROX SYBR serial dilutions that spanned the range of the target gene amplification from environmental MasterMix blue dTTP (Eurogentec, Fremont, CA) for SYBR green PCR and PrimeTime Gene Expression Master Mix (Integrated DNA Technologies, Coralville, IA) for TaqMan PCR.

    Article Title: Acquisition of fluoroquinolone resistance leads to increased biofilm formation and pathogenicity in Campylobacter jejuni
    Article Snippet: .. Plasmid isolation was carried out on 20 ml of overnight cultures using the QIAgen MiniPrep kit and DNA concentrations were normalized using a NanoDrop spectrophotometer. ..

    Software:

    Article Title: The effects of in utero bisphenol A exposure on ovarian follicle numbers and steroidogenesis in the F1 and F2 generations of mice
    Article Snippet: Total RNA was extracted from the ovaries using the RNeasy Mini Kit (Qiagen, Inc., Valencia, CA) and quantified with the Nanodrop® spectrophotometer following manufacturer's instructions. .. Total RNA (100 ng per sample) was then reverse transcribed to complementary DNA (cDNA) using the iScript RT kit (Bio-Rad Laboratories, Inc., Hercules, CA) and subsequently subjected to qPCR using the CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Inc., Hercules, CA) and accompanying CFX Manager Software as per manufacturer's recommendations.

    Article Title: Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine
    Article Snippet: After RNA quantification using a NanoDropTM spectrophotometer, 500 ng was processed to obtain cDNA by High Capacity cDNA Reverse Transcription (#4368814; Applied Biosystems). .. An Applied Biosystems Prism 7900HT Sequence Detection System was used, with the Sequence Detector Software (SDS version 2.0; Applied Biosystems) ( ).

    Real-time Polymerase Chain Reaction:

    Article Title: Synthetic presentation of noncanonical Wnt5a motif promotes mechanosensing-dependent differentiation of stem cells and regeneration
    Article Snippet: The RNA concentration was measured using a NanoDrop One spectrophotometer (NanoDrop Technologies, Waltham, Massachusetts, USA). .. Total RNA (1 μg) was reverse transcribed into cDNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). qPCR was performed on an Applied Biosystems StepOnePlus Real-Time PCR System with TaqMan primers and probes (Applied Biosciences, Waltham, Massachusetts, USA) specific for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and other osteogenic genes, including those encoding RUNX2, ALP, and type I collagen.

    Article Title: The effects of in utero bisphenol A exposure on ovarian follicle numbers and steroidogenesis in the F1 and F2 generations of mice
    Article Snippet: Paragraph title: Quantitative real-time polymerase chain reaction ... Total RNA was extracted from the ovaries using the RNeasy Mini Kit (Qiagen, Inc., Valencia, CA) and quantified with the Nanodrop® spectrophotometer following manufacturer's instructions.

    Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function
    Article Snippet: .. Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system. ..

    Article Title: Characterizing the soil microbiome and quantifying antibiotic resistance gene dynamics in agricultural soil following swine CAFO manure application
    Article Snippet: Paragraph title: qPCR for gene targets ... Plasmid copy number was calculated using the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher, Waltham, MA).

    Article Title: Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine
    Article Snippet: After RNA quantification using a NanoDropTM spectrophotometer, 500 ng was processed to obtain cDNA by High Capacity cDNA Reverse Transcription (#4368814; Applied Biosystems). .. We evaluated by quantitative real-time PCR the following genes: paired box (Pax) 7 (#4331182, Hs00242962_m1); myogenic differentiation (MyoD) 1 (#4331182, Hs00159528_m1), myogenin (#4331182, Hs 01072232GEXper-design_m1).

    RNA Extraction:

    Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function
    Article Snippet: Paragraph title: RNA, extraction quantification and qPCR ... Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system.

    Article Title: Mapping Semaphorins and Netrins in the Pathogenesis of Human Thoracic Aortic Aneurysms
    Article Snippet: RNA Isolation RNA extraction from TAA or healthy tissue was done by using RNEasy fibrous tissue kit according to the manufacturer’s instructions (74704, Qiagen, Hilden, Germany). .. Concentration and quality of RNA were verified by a NanoDrop One microvolume UV-vis spectrophotometer (ND-ONEW-W, Thermofisher Scientific, Wilmington, DE, USA).

    Article Title: Induction of RNAi Core Machinery’s Gene Expression by Exogenous dsRNA and the Effects of Pre-exposure to dsRNA on the Gene Silencing Efficiency in the Pea Aphid (Acyrthosiphon pisum)
    Article Snippet: Paragraph title: RNA Extraction, cDNA Synthesis and Cloning ... The RNA was then quantified using a NanoDrop One microvolume UV-Vis spectrophotometer (Thermo Scientific, Wilmington, MA, United States).

    Article Title: Transcriptome analysis of Inbred Long Sleep and Inbred Short Sleep mice
    Article Snippet: Paragraph title: RNA extraction ... Total RNA, from whole brains (WB, n=6, 3/strain) and striatum (ST, n=6, 3/strain) was extracted using RNeasy midi kits (Qiagen, Valencia, CA), and quantity and quality were determined using a NanoDrop™ spectrophotometer (Thermo Fisher Scientific, Wilmington, DE) and Agilent 2100 BioAnalyzer™ (Agilent Technologies, Santa Clara, CA).

    Electrophoresis:

    Article Title: Induction of RNAi Core Machinery’s Gene Expression by Exogenous dsRNA and the Effects of Pre-exposure to dsRNA on the Gene Silencing Efficiency in the Pea Aphid (Acyrthosiphon pisum)
    Article Snippet: The RNA was then quantified using a NanoDrop One microvolume UV-Vis spectrophotometer (Thermo Scientific, Wilmington, MA, United States). .. The purities of the RNA samples were assessed at absorbance ratios of OD260/280 and OD260/230 , and then the integrity levels of the samples were evaluated by electrophoresis using 1% (w /v ) agarose gels.

    ALP Assay:

    Article Title: Synthetic presentation of noncanonical Wnt5a motif promotes mechanosensing-dependent differentiation of stem cells and regeneration
    Article Snippet: The RNA concentration was measured using a NanoDrop One spectrophotometer (NanoDrop Technologies, Waltham, Massachusetts, USA). .. Total RNA (1 μg) was reverse transcribed into cDNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). qPCR was performed on an Applied Biosystems StepOnePlus Real-Time PCR System with TaqMan primers and probes (Applied Biosciences, Waltham, Massachusetts, USA) specific for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and other osteogenic genes, including those encoding RUNX2, ALP, and type I collagen.

    Spectrophotometry:

    Article Title: Detection of KRAS mutation via ligation-initiated LAMP reaction
    Article Snippet: .. The DNA concentration was determined by NanoDrop One Microvolume UV-vis spectrophotometer (Thermo Scientific, USA). ..

    Article Title: Synthetic presentation of noncanonical Wnt5a motif promotes mechanosensing-dependent differentiation of stem cells and regeneration
    Article Snippet: .. The RNA concentration was measured using a NanoDrop One spectrophotometer (NanoDrop Technologies, Waltham, Massachusetts, USA). .. Total RNA (1 μg) was reverse transcribed into cDNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). qPCR was performed on an Applied Biosystems StepOnePlus Real-Time PCR System with TaqMan primers and probes (Applied Biosciences, Waltham, Massachusetts, USA) specific for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and other osteogenic genes, including those encoding RUNX2, ALP, and type I collagen.

    Article Title: The effects of in utero bisphenol A exposure on ovarian follicle numbers and steroidogenesis in the F1 and F2 generations of mice
    Article Snippet: .. Total RNA was extracted from the ovaries using the RNeasy Mini Kit (Qiagen, Inc., Valencia, CA) and quantified with the Nanodrop® spectrophotometer following manufacturer's instructions. .. Total RNA (100 ng per sample) was then reverse transcribed to complementary DNA (cDNA) using the iScript RT kit (Bio-Rad Laboratories, Inc., Hercules, CA) and subsequently subjected to qPCR using the CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Inc., Hercules, CA) and accompanying CFX Manager Software as per manufacturer's recommendations.

    Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function
    Article Snippet: .. Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system. ..

    Article Title: Full-Length Transcriptome Survey and Expression Analysis of Parasitoid Wasp Chouioia cunea upon Exposure to 1-Dodecene
    Article Snippet: .. The concentration and quality of RNA were determined using a Nanodrop spectrophotometer. .. The integrity of the RNA was detected accurately using an Agilent 2100 device (Tables and ).

    Article Title: Comparison of methods for miRNA isolation and quantification from ovine plasma
    Article Snippet: .. NanoDrop spectrophotometer A NanoDrop One UV-Vis spectrophotometer (ThermoScientific) was used for RNA assessment this utilises the absorbance of light by nucleic acids to determine their concentration. .. Prior to measurement, the NanoDrop was blanked with 2 µl of the same nuclease-free water that the RNA was eluted in.

    Article Title: Characterizing the soil microbiome and quantifying antibiotic resistance gene dynamics in agricultural soil following swine CAFO manure application
    Article Snippet: .. Plasmid copy number was calculated using the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher, Waltham, MA). .. Each reaction was prepared using 12.5 μl of Takyon ROX SYBR serial dilutions that spanned the range of the target gene amplification from environmental MasterMix blue dTTP (Eurogentec, Fremont, CA) for SYBR green PCR and PrimeTime Gene Expression Master Mix (Integrated DNA Technologies, Coralville, IA) for TaqMan PCR.

    Article Title: Altered Gut Microbiota and Compositional Changes in Firmicutes and Proteobacteria in Mexican Undernourished and Obese Children
    Article Snippet: .. DNA concentrations were measured by using Nano Drop V3.8.1 spectrophotometer. .. The V3–V4 hypervariable region of bacterial 16S rRNA gene was amplified by GeneAmp PCR system 9700 (Applied Biosystems).

    Article Title: Acquisition of fluoroquinolone resistance leads to increased biofilm formation and pathogenicity in Campylobacter jejuni
    Article Snippet: .. Plasmid isolation was carried out on 20 ml of overnight cultures using the QIAgen MiniPrep kit and DNA concentrations were normalized using a NanoDrop spectrophotometer. ..

    Article Title: Mapping Semaphorins and Netrins in the Pathogenesis of Human Thoracic Aortic Aneurysms
    Article Snippet: .. Concentration and quality of RNA were verified by a NanoDrop One microvolume UV-vis spectrophotometer (ND-ONEW-W, Thermofisher Scientific, Wilmington, DE, USA). .. RNA Sequencing RNA was isolated from 10 human aortas and 3 internal thoracic artery samples then processed using Clontech Low Input Kit according to manufacturer’s instructions to prepare RNA-Seq libraries.

    Article Title: Induction of RNAi Core Machinery’s Gene Expression by Exogenous dsRNA and the Effects of Pre-exposure to dsRNA on the Gene Silencing Efficiency in the Pea Aphid (Acyrthosiphon pisum)
    Article Snippet: .. The RNA was then quantified using a NanoDrop One microvolume UV-Vis spectrophotometer (Thermo Scientific, Wilmington, MA, United States). .. The purities of the RNA samples were assessed at absorbance ratios of OD260/280 and OD260/230 , and then the integrity levels of the samples were evaluated by electrophoresis using 1% (w /v ) agarose gels.

    Article Title: Extracellular Guanosine 5′-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine
    Article Snippet: .. After RNA quantification using a NanoDropTM spectrophotometer, 500 ng was processed to obtain cDNA by High Capacity cDNA Reverse Transcription (#4368814; Applied Biosystems). .. We evaluated by quantitative real-time PCR the following genes: paired box (Pax) 7 (#4331182, Hs00242962_m1); myogenic differentiation (MyoD) 1 (#4331182, Hs00159528_m1), myogenin (#4331182, Hs 01072232GEXper-design_m1).

    Article Title: Biological Effects of Functionalizing Copolymer Scaffolds with Nanodiamond Particles
    Article Snippet: .. The protein and DNA level were also measured directly on a Nanodrop® Spectrophotometer as described above. ..

    Article Title: Transcriptome analysis of Inbred Long Sleep and Inbred Short Sleep mice
    Article Snippet: .. Total RNA, from whole brains (WB, n=6, 3/strain) and striatum (ST, n=6, 3/strain) was extracted using RNeasy midi kits (Qiagen, Valencia, CA), and quantity and quality were determined using a NanoDrop™ spectrophotometer (Thermo Fisher Scientific, Wilmington, DE) and Agilent 2100 BioAnalyzer™ (Agilent Technologies, Santa Clara, CA). ..

    Article Title: Differential expression of heat shock proteins and antioxidant enzymes in response to temperature, starvation, and parasitism in the Carob moth larvae, Ectomyelois ceratoniae (Lepidoptera: Pyralidae)
    Article Snippet: .. The concentration and quality of dsRNAs were assessed with a Nanodrop spectrophotometer. .. Quantitative PCR (RT-qPCR) RNA and cDNA was prepared from pools of 10 larvae for each stress and control treatment.

    Concentration Assay:

    Article Title: Detection of KRAS mutation via ligation-initiated LAMP reaction
    Article Snippet: .. The DNA concentration was determined by NanoDrop One Microvolume UV-vis spectrophotometer (Thermo Scientific, USA). ..

    Article Title: Synthetic presentation of noncanonical Wnt5a motif promotes mechanosensing-dependent differentiation of stem cells and regeneration
    Article Snippet: .. The RNA concentration was measured using a NanoDrop One spectrophotometer (NanoDrop Technologies, Waltham, Massachusetts, USA). .. Total RNA (1 μg) was reverse transcribed into cDNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). qPCR was performed on an Applied Biosystems StepOnePlus Real-Time PCR System with TaqMan primers and probes (Applied Biosciences, Waltham, Massachusetts, USA) specific for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and other osteogenic genes, including those encoding RUNX2, ALP, and type I collagen.

    Article Title: Full-Length Transcriptome Survey and Expression Analysis of Parasitoid Wasp Chouioia cunea upon Exposure to 1-Dodecene
    Article Snippet: .. The concentration and quality of RNA were determined using a Nanodrop spectrophotometer. .. The integrity of the RNA was detected accurately using an Agilent 2100 device (Tables and ).

    Article Title: Comparison of methods for miRNA isolation and quantification from ovine plasma
    Article Snippet: .. NanoDrop spectrophotometer A NanoDrop One UV-Vis spectrophotometer (ThermoScientific) was used for RNA assessment this utilises the absorbance of light by nucleic acids to determine their concentration. .. Prior to measurement, the NanoDrop was blanked with 2 µl of the same nuclease-free water that the RNA was eluted in.

    Article Title: Mapping Semaphorins and Netrins in the Pathogenesis of Human Thoracic Aortic Aneurysms
    Article Snippet: .. Concentration and quality of RNA were verified by a NanoDrop One microvolume UV-vis spectrophotometer (ND-ONEW-W, Thermofisher Scientific, Wilmington, DE, USA). .. RNA Sequencing RNA was isolated from 10 human aortas and 3 internal thoracic artery samples then processed using Clontech Low Input Kit according to manufacturer’s instructions to prepare RNA-Seq libraries.

    Article Title: Differential expression of heat shock proteins and antioxidant enzymes in response to temperature, starvation, and parasitism in the Carob moth larvae, Ectomyelois ceratoniae (Lepidoptera: Pyralidae)
    Article Snippet: .. The concentration and quality of dsRNAs were assessed with a Nanodrop spectrophotometer. .. Quantitative PCR (RT-qPCR) RNA and cDNA was prepared from pools of 10 larvae for each stress and control treatment.

    DNA Purification:

    Article Title: Characterizing the soil microbiome and quantifying antibiotic resistance gene dynamics in agricultural soil following swine CAFO manure application
    Article Snippet: Plasmids were transformed into Escherichia coli One Shot TOP10 Cells (Thermofisher, Waltham, MA) using the manufacturer’s instructions and were extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega, Madison, WI) following the manufacturer’s instructions. .. Plasmid copy number was calculated using the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher, Waltham, MA).

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    Thermo Fisher nano drop 2000 spectrometer
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    Thermo Fisher nano drop spectrometer
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