nano drop 2000 spectrometer  (Thermo Fisher)


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    Name:
    NanoDrop 2000 Spectrophotometer
    Description:
    Thermo Scientific NanoDrop 2000 and 2000c are full spectrum UV Vis spectrophotometers used to quantify and assess purity of DNA RNA Protein and more The NanoDrop 2000 and 2000c are the only microvolume spectrophotometers with patented sample retention technology that measure sample volumes as small as 0 5μL
    Catalog Number:
    nd-2000
    Price:
    None
    Applications:
    Industrial & Applied Science|Spectroscopy Elemental Isotope Analysis
    Category:
    Instruments and Equipment
    Buy from Supplier


    Structured Review

    Thermo Fisher nano drop 2000 spectrometer
    Thermo Scientific NanoDrop 2000 and 2000c are full spectrum UV Vis spectrophotometers used to quantify and assess purity of DNA RNA Protein and more The NanoDrop 2000 and 2000c are the only microvolume spectrophotometers with patented sample retention technology that measure sample volumes as small as 0 5μL
    https://www.bioz.com/result/nano drop 2000 spectrometer/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nano drop 2000 spectrometer - by Bioz Stars, 2020-03
    99/100 stars

    Related Products / Commonly Used Together

    gel electrophoresis
    sequencing dna

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    Related Articles

    Centrifugation:

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: RNA Isolation For RNA isolation, A. glaucus (strain CCHA) was grown in PDB liquid medium with 3 M NaCl and harvested by centrifugation after 4 days. .. The quality and quantity of total RNA were analyzed using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis.

    Article Title: MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair ▿MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair ▿ †
    Article Snippet: Immunoprecipitated DNA was purified by the standard phenol chloroform procedure , and DNA was quantified with a NanoDrop 2000 spectrometer (Thermo Scientific, Inc.). .. The supernatants were collected at each step after extraction buffer treatment, and supernatants were cleared by centrifugation.

    Amplification:

    Article Title: GRK3 is a direct target of CREB activation and regulates neuroendocrine differentiation of prostate cancer cells
    Article Snippet: The RNA concentration and purity were measured by NanoDrop 2000 UV-vis Spectrophotometer (Thermo Scientific, USA). .. PCR-based amplification was performed using the following primers.

    Article Title: Characterization of Diversity and Probiotic Efficiency of the Autochthonous Lactic Acid Bacteria in the Fermentation of Selected Raw Fruit and Vegetable Juices
    Article Snippet: The final concentration and purification of DNA were determined using a NanoDrop 2000 UV-vis spectrophotometer (Thermo Scientific, Wilmington, DE, United States), and DNA quality was checked via 1% agarose gel electrophoresis. .. The V3 and V4 hypervariable regions of the bacteria 16S rRNA genes were amplified with primers 338F (5′- ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTA AT-3′) using a thermocycler polymerase chain reaction (PCR) system (GeneAmp 9700; ABI, Carlsbad, CA, United States).

    Synthesized:

    Article Title: CDX2 increases SLC7A7 expression and proliferation of pig intestinal epithelial cells
    Article Snippet: The concentration and purity of the RNA sample was determined by measuring the optical density using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, MA, USA). .. The cDNA was synthesized from 2 μg of DNA-free RNA using N10 primers (Sangon, Shanghai, China) and Moloney murine leukemia virus reverse transcriptase (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions.

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: Briefly, the first strand cDNA was synthesized from 5 μ g total RNA using SuperScript III (Clontech, Mountain View, USA) with SMART IV oligonucleotide primer and CDS III/3′ PCR primer ( ). .. The concentration and yield of the ds cDNA fractions were estimated using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis.

    Article Title: An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoids
    Article Snippet: .. The concentration of total RNA was measured on a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) and the integrity was examined on agarose gels. cDNA was synthesized from 500 ng of total RNA with RevertAid H Minus Reverse Transcriptase (ThermoFisher Scientific). .. Quantitative real-time PCR (qRT-PCR) was performed on a CFX Connect real-time system (Bio-Rad) using iTaqTM Universal SYBR Green Supermix kits (Bio-Rad).

    Picogreen Assay:

    Article Title: Comparative Study of Seven Commercial Kits for Human DNA Extraction from Urine Samples Suitable for DNA Biomarker-Based Public Health Studies
    Article Snippet: .. The yield was first quantified by two different methods: the NanoDrop 2000 spectrophotometer and Quant-iT PicoGreen assay ( ) Samples have been grouped by sex, as it is known that female urine contains more cells and higher amounts of DNA than male urine., The DNA yield/ml urine, obtained with NanoDrop, ranged from 19 to 7182 ng/ml for the female urine and from 6 to 7128 ng/ml for the male urine. .. Here, it must be taken into account that Qiagen kits are using carrier RNA to improve the recovery of DNA from urine samples.

    Construct:

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: Library Construction in E. coli DH5α A cDNA library was constructed using the SMART cDNA Library Construction Kit (Clontech, Mountain View, USA) according to the manufacturer's instructions with minor modifications. .. The concentration and yield of the ds cDNA fractions were estimated using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis.

    Concentration Assay:

    Article Title: CDX2 increases SLC7A7 expression and proliferation of pig intestinal epithelial cells
    Article Snippet: .. The concentration and purity of the RNA sample was determined by measuring the optical density using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, MA, USA). .. The integrity of the RNA was assessed with 1.5% agarose gel electrophoresis.

    Article Title: Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin
    Article Snippet: .. The concentration of extracted RNA was confirmed using a NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE). .. All mouse primers (Supplementary Table ) were purchased from Eurofins Scientific (Eurofins, Luxembourg). cDNA synthesis was performed using Superscript III RT, RNaseOUT, Ribonuclease inhibitor, Oligo(dT)12–18, 10 mM dNTP mix, 5X first-strand buffer, and 0.1 M DTT, following manufacturer’s instructions (Thermo Fischer Scientific, Waltham, MA).

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: .. The concentration and yield of the ds cDNA fractions were estimated using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis. .. The ds cDNA longer than 700 bp was pooled, precipitated, and used for ligation reactions with modified vector pYES2G digested by Sfi I [ ].

    Article Title: GRK3 is a direct target of CREB activation and regulates neuroendocrine differentiation of prostate cancer cells
    Article Snippet: .. The RNA concentration and purity were measured by NanoDrop 2000 UV-vis Spectrophotometer (Thermo Scientific, USA). .. 3μg of total RNA was used to generate cDNA using the iScript Reverse Transcription Supermix (Bio-Rad, USA).

    Article Title: An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoids
    Article Snippet: .. The concentration of total RNA was measured on a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) and the integrity was examined on agarose gels. cDNA was synthesized from 500 ng of total RNA with RevertAid H Minus Reverse Transcriptase (ThermoFisher Scientific). .. Quantitative real-time PCR (qRT-PCR) was performed on a CFX Connect real-time system (Bio-Rad) using iTaqTM Universal SYBR Green Supermix kits (Bio-Rad).

    Article Title: Characterization of Diversity and Probiotic Efficiency of the Autochthonous Lactic Acid Bacteria in the Fermentation of Selected Raw Fruit and Vegetable Juices
    Article Snippet: .. The final concentration and purification of DNA were determined using a NanoDrop 2000 UV-vis spectrophotometer (Thermo Scientific, Wilmington, DE, United States), and DNA quality was checked via 1% agarose gel electrophoresis. .. The V3 and V4 hypervariable regions of the bacteria 16S rRNA genes were amplified with primers 338F (5′- ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTA AT-3′) using a thermocycler polymerase chain reaction (PCR) system (GeneAmp 9700; ABI, Carlsbad, CA, United States).

    Article Title: Exploiting 1,2,3-Triazolium Ionic Liquids for Synthesis of Tryptanthrin and Chemoselective Extraction of Copper(II) Ions and Histidine-Containing Peptides
    Article Snippet: .. The upper layer was then measured to determine the concentration of copper chloride in the aqueous layer using the NanoDrop 2000 UV-Vis spectrophotometer. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin
    Article Snippet: The concentration of extracted RNA was confirmed using a NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE). .. Real-time PCR was performed using SYBR™ Green PCR Master Mix and detected using 7300 Real-Time PCR System (Thermo Fischer Scientific, Waltham, MA).

    Article Title: GRK3 is a direct target of CREB activation and regulates neuroendocrine differentiation of prostate cancer cells
    Article Snippet: Paragraph title: Reverse transcription and quantitative real-time PCR ... The RNA concentration and purity were measured by NanoDrop 2000 UV-vis Spectrophotometer (Thermo Scientific, USA).

    Article Title: An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoids
    Article Snippet: Paragraph title: RNA extraction and quantitative real-time PCR analysis ... The concentration of total RNA was measured on a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) and the integrity was examined on agarose gels. cDNA was synthesized from 500 ng of total RNA with RevertAid H Minus Reverse Transcriptase (ThermoFisher Scientific).

    Cell Fractionation:

    Article Title: MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair ▿MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair ▿ †
    Article Snippet: Immunoprecipitated DNA was purified by the standard phenol chloroform procedure , and DNA was quantified with a NanoDrop 2000 spectrometer (Thermo Scientific, Inc.). .. To determine how tightly hMOF is bound with DNA, cells with and without exposure to IR were washed with ice-cold PBS, and cell fractionation was carried out by four consecutive extractions with increasing detergent concentrations as described previously ( ).

    Modification:

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: The concentration and yield of the ds cDNA fractions were estimated using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis. .. The ds cDNA longer than 700 bp was pooled, precipitated, and used for ligation reactions with modified vector pYES2G digested by Sfi I [ ].

    Ligation:

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: The concentration and yield of the ds cDNA fractions were estimated using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis. .. The ds cDNA longer than 700 bp was pooled, precipitated, and used for ligation reactions with modified vector pYES2G digested by Sfi I [ ].

    Polymerase Chain Reaction:

    Article Title: Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin
    Article Snippet: The concentration of extracted RNA was confirmed using a NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE). .. Real-time PCR was performed using SYBR™ Green PCR Master Mix and detected using 7300 Real-Time PCR System (Thermo Fischer Scientific, Waltham, MA).

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: Briefly, the first strand cDNA was synthesized from 5 μ g total RNA using SuperScript III (Clontech, Mountain View, USA) with SMART IV oligonucleotide primer and CDS III/3′ PCR primer ( ). .. The concentration and yield of the ds cDNA fractions were estimated using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis.

    Article Title: GRK3 is a direct target of CREB activation and regulates neuroendocrine differentiation of prostate cancer cells
    Article Snippet: The RNA concentration and purity were measured by NanoDrop 2000 UV-vis Spectrophotometer (Thermo Scientific, USA). .. PCR-based amplification was performed using the following primers.

    Article Title: Characterization of Diversity and Probiotic Efficiency of the Autochthonous Lactic Acid Bacteria in the Fermentation of Selected Raw Fruit and Vegetable Juices
    Article Snippet: The final concentration and purification of DNA were determined using a NanoDrop 2000 UV-vis spectrophotometer (Thermo Scientific, Wilmington, DE, United States), and DNA quality was checked via 1% agarose gel electrophoresis. .. The V3 and V4 hypervariable regions of the bacteria 16S rRNA genes were amplified with primers 338F (5′- ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTA AT-3′) using a thermocycler polymerase chain reaction (PCR) system (GeneAmp 9700; ABI, Carlsbad, CA, United States).

    Nucleic Acid Electrophoresis:

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: .. The quality and quantity of total RNA were analyzed using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis. .. Library Construction in E. coli DH5α A cDNA library was constructed using the SMART cDNA Library Construction Kit (Clontech, Mountain View, USA) according to the manufacturer's instructions with minor modifications.

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: .. The concentration and yield of the ds cDNA fractions were estimated using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis. .. The ds cDNA longer than 700 bp was pooled, precipitated, and used for ligation reactions with modified vector pYES2G digested by Sfi I [ ].

    In Vivo:

    Article Title: MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair ▿MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair ▿ †
    Article Snippet: Total DNA from 107 cells, coimmunoprecipitated with MOF or retinoblastoma (Rb) antibody after in vivo cross-linking, was performed by the standard procedure described previously ( , ). .. Immunoprecipitated DNA was purified by the standard phenol chloroform procedure , and DNA was quantified with a NanoDrop 2000 spectrometer (Thermo Scientific, Inc.).

    Irradiation:

    Article Title: MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair ▿MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair ▿ †
    Article Snippet: To determine the retention of MOF with DNA, 107 cells growing in exponential phase were irradiated and fixed with 4% formaldehyde at different times postirradiation. .. Immunoprecipitated DNA was purified by the standard phenol chloroform procedure , and DNA was quantified with a NanoDrop 2000 spectrometer (Thermo Scientific, Inc.).

    Isolation:

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: Paragraph title: 2.2. RNA Isolation ... The quality and quantity of total RNA were analyzed using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis.

    Article Title: CDX2 increases SLC7A7 expression and proliferation of pig intestinal epithelial cells
    Article Snippet: RNA extraction and first-strand cDNA synthesis Total RNA was isolated from the duodenum, jejunum, and ileum mixture of the piglets or IPEC-1 cells using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA). .. The concentration and purity of the RNA sample was determined by measuring the optical density using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, MA, USA).

    Article Title: Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin
    Article Snippet: Paragraph title: RNA isolation and qRT-PCR ... The concentration of extracted RNA was confirmed using a NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE).

    Purification:

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: Total RNA was isolated from 200 mg homogenized tissue using a Norgen RNA Purification Kit (Norgen Biotek Corp., Ontario, Canada). .. The quality and quantity of total RNA were analyzed using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis.

    Article Title: MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair ▿MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair ▿ †
    Article Snippet: .. Immunoprecipitated DNA was purified by the standard phenol chloroform procedure , and DNA was quantified with a NanoDrop 2000 spectrometer (Thermo Scientific, Inc.). ..

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: The purified ds cDNA was digested with Sfi I at 50°C for 4 h, 2 μ L 1% xylene cyanol dye was added, and cDNA size fractionation was performed using CHROMA SPIN-400 (Clontech, Mountain View, USA). .. The concentration and yield of the ds cDNA fractions were estimated using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis.

    Article Title: Characterization of Diversity and Probiotic Efficiency of the Autochthonous Lactic Acid Bacteria in the Fermentation of Selected Raw Fruit and Vegetable Juices
    Article Snippet: .. The final concentration and purification of DNA were determined using a NanoDrop 2000 UV-vis spectrophotometer (Thermo Scientific, Wilmington, DE, United States), and DNA quality was checked via 1% agarose gel electrophoresis. .. The V3 and V4 hypervariable regions of the bacteria 16S rRNA genes were amplified with primers 338F (5′- ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTA AT-3′) using a thermocycler polymerase chain reaction (PCR) system (GeneAmp 9700; ABI, Carlsbad, CA, United States).

    Quantitative RT-PCR:

    Article Title: Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin
    Article Snippet: Paragraph title: RNA isolation and qRT-PCR ... The concentration of extracted RNA was confirmed using a NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE).

    Article Title: An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoids
    Article Snippet: The concentration of total RNA was measured on a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) and the integrity was examined on agarose gels. cDNA was synthesized from 500 ng of total RNA with RevertAid H Minus Reverse Transcriptase (ThermoFisher Scientific). .. Quantitative real-time PCR (qRT-PCR) was performed on a CFX Connect real-time system (Bio-Rad) using iTaqTM Universal SYBR Green Supermix kits (Bio-Rad).

    cDNA Library Assay:

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: Library Construction in E. coli DH5α A cDNA library was constructed using the SMART cDNA Library Construction Kit (Clontech, Mountain View, USA) according to the manufacturer's instructions with minor modifications. .. The concentration and yield of the ds cDNA fractions were estimated using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis.

    Plasmid Preparation:

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: The concentration and yield of the ds cDNA fractions were estimated using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis. .. The ds cDNA longer than 700 bp was pooled, precipitated, and used for ligation reactions with modified vector pYES2G digested by Sfi I [ ].

    SYBR Green Assay:

    Article Title: Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin
    Article Snippet: The concentration of extracted RNA was confirmed using a NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE). .. Real-time PCR was performed using SYBR™ Green PCR Master Mix and detected using 7300 Real-Time PCR System (Thermo Fischer Scientific, Waltham, MA).

    Article Title: An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoids
    Article Snippet: The concentration of total RNA was measured on a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) and the integrity was examined on agarose gels. cDNA was synthesized from 500 ng of total RNA with RevertAid H Minus Reverse Transcriptase (ThermoFisher Scientific). .. Quantitative real-time PCR (qRT-PCR) was performed on a CFX Connect real-time system (Bio-Rad) using iTaqTM Universal SYBR Green Supermix kits (Bio-Rad).

    RNA Extraction:

    Article Title: CDX2 increases SLC7A7 expression and proliferation of pig intestinal epithelial cells
    Article Snippet: Paragraph title: RNA extraction and first-strand cDNA synthesis ... The concentration and purity of the RNA sample was determined by measuring the optical density using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, MA, USA).

    Article Title: An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoids
    Article Snippet: Paragraph title: RNA extraction and quantitative real-time PCR analysis ... The concentration of total RNA was measured on a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) and the integrity was examined on agarose gels. cDNA was synthesized from 500 ng of total RNA with RevertAid H Minus Reverse Transcriptase (ThermoFisher Scientific).

    Agarose Gel Electrophoresis:

    Article Title: CDX2 increases SLC7A7 expression and proliferation of pig intestinal epithelial cells
    Article Snippet: The concentration and purity of the RNA sample was determined by measuring the optical density using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, MA, USA). .. The integrity of the RNA was assessed with 1.5% agarose gel electrophoresis.

    Article Title: Characterization of Diversity and Probiotic Efficiency of the Autochthonous Lactic Acid Bacteria in the Fermentation of Selected Raw Fruit and Vegetable Juices
    Article Snippet: .. The final concentration and purification of DNA were determined using a NanoDrop 2000 UV-vis spectrophotometer (Thermo Scientific, Wilmington, DE, United States), and DNA quality was checked via 1% agarose gel electrophoresis. .. The V3 and V4 hypervariable regions of the bacteria 16S rRNA genes were amplified with primers 338F (5′- ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTA AT-3′) using a thermocycler polymerase chain reaction (PCR) system (GeneAmp 9700; ABI, Carlsbad, CA, United States).

    In Vitro:

    Article Title: The sustained-release behavior and in vitro and in vivo transfection of pEGFP-loaded core-shell-structured chitosan-based composite particles
    Article Snippet: Paragraph title: In vitro sustained-release behavior of pEGFP -loaded TACS-HBC composite particles ... The amount of pEGFP released was determined by UV-visible absorption method, as measured with the NanoDrop 2000 Micro UV spectrophotometer.

    Spectrophotometry:

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: .. The quality and quantity of total RNA were analyzed using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis. .. Library Construction in E. coli DH5α A cDNA library was constructed using the SMART cDNA Library Construction Kit (Clontech, Mountain View, USA) according to the manufacturer's instructions with minor modifications.

    Article Title: CDX2 increases SLC7A7 expression and proliferation of pig intestinal epithelial cells
    Article Snippet: .. The concentration and purity of the RNA sample was determined by measuring the optical density using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, MA, USA). .. The integrity of the RNA was assessed with 1.5% agarose gel electrophoresis.

    Article Title: Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin
    Article Snippet: .. The concentration of extracted RNA was confirmed using a NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE). .. All mouse primers (Supplementary Table ) were purchased from Eurofins Scientific (Eurofins, Luxembourg). cDNA synthesis was performed using Superscript III RT, RNaseOUT, Ribonuclease inhibitor, Oligo(dT)12–18, 10 mM dNTP mix, 5X first-strand buffer, and 0.1 M DTT, following manufacturer’s instructions (Thermo Fischer Scientific, Waltham, MA).

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: .. The concentration and yield of the ds cDNA fractions were estimated using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis. .. The ds cDNA longer than 700 bp was pooled, precipitated, and used for ligation reactions with modified vector pYES2G digested by Sfi I [ ].

    Article Title: GRK3 is a direct target of CREB activation and regulates neuroendocrine differentiation of prostate cancer cells
    Article Snippet: .. The RNA concentration and purity were measured by NanoDrop 2000 UV-vis Spectrophotometer (Thermo Scientific, USA). .. 3μg of total RNA was used to generate cDNA using the iScript Reverse Transcription Supermix (Bio-Rad, USA).

    Article Title: An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoids
    Article Snippet: .. The concentration of total RNA was measured on a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) and the integrity was examined on agarose gels. cDNA was synthesized from 500 ng of total RNA with RevertAid H Minus Reverse Transcriptase (ThermoFisher Scientific). .. Quantitative real-time PCR (qRT-PCR) was performed on a CFX Connect real-time system (Bio-Rad) using iTaqTM Universal SYBR Green Supermix kits (Bio-Rad).

    Article Title: Different subtype strains of Akkermansia muciniphila abundantly colonize in southern China
    Article Snippet: .. Figure S2 OD600 of Akkermansia muciniphila in medium was measured on a Nanodrop 2000 UV‐Vis spectrophotometer from 8 to 48 h. The doubling time was 1·4 h. Click here for additional data file. ..

    Article Title: Comparative Study of Seven Commercial Kits for Human DNA Extraction from Urine Samples Suitable for DNA Biomarker-Based Public Health Studies
    Article Snippet: .. The yield was first quantified by two different methods: the NanoDrop 2000 spectrophotometer and Quant-iT PicoGreen assay ( ) Samples have been grouped by sex, as it is known that female urine contains more cells and higher amounts of DNA than male urine., The DNA yield/ml urine, obtained with NanoDrop, ranged from 19 to 7182 ng/ml for the female urine and from 6 to 7128 ng/ml for the male urine. .. Here, it must be taken into account that Qiagen kits are using carrier RNA to improve the recovery of DNA from urine samples.

    Article Title: Saponins enhance exon skipping of 2′-O-methyl phosphorothioate oligonucleotide in vitro and in vivo
    Article Snippet: .. The absorbance of saponin formulated 2′-OMePS was determined with NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). .. The saponin/2′-OMePS complex solution containing 1 µg of 2′-OMePS was prepared at different weight ratios of saponin/2′-OMePS in 100 µL 0.9% saline and analyzed using transmission electron microscopy (TEM) with AMT-XR80S-B wide angle side Mount 8M Pixel, CCD Camera (JEM-1400Plus Transmission Electron Microscope by JEOL USA, Inc., Peabody, MA, USA).

    Article Title: The sustained-release behavior and in vitro and in vivo transfection of pEGFP-loaded core-shell-structured chitosan-based composite particles
    Article Snippet: .. The amount of pEGFP released was determined by UV-visible absorption method, as measured with the NanoDrop 2000 Micro UV spectrophotometer. .. The release rate was characterized as Release rate = C s a m p l e C t o t a l × 100 % , (3) where C sample is the concentration of pEGFP in the sample; and C total is the theoretical concentration of pEGFP , provided that all pEGFP -loaded in particles have been released into the TE buffer solution.

    Article Title: Exploiting 1,2,3-Triazolium Ionic Liquids for Synthesis of Tryptanthrin and Chemoselective Extraction of Copper(II) Ions and Histidine-Containing Peptides
    Article Snippet: .. The upper layer was then measured to determine the concentration of copper chloride in the aqueous layer using the NanoDrop 2000 UV-Vis spectrophotometer. ..

    Immunoprecipitation:

    Article Title: MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair ▿MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair ▿ †
    Article Snippet: .. Immunoprecipitated DNA was purified by the standard phenol chloroform procedure , and DNA was quantified with a NanoDrop 2000 spectrometer (Thermo Scientific, Inc.). ..

    Fractionation:

    Article Title: Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
    Article Snippet: The purified ds cDNA was digested with Sfi I at 50°C for 4 h, 2 μ L 1% xylene cyanol dye was added, and cDNA size fractionation was performed using CHROMA SPIN-400 (Clontech, Mountain View, USA). .. The concentration and yield of the ds cDNA fractions were estimated using a NanoDrop 2000 UV-Vis spectrophotometer (Thermo, Wilmington, USA) and gel electrophoresis.

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  • 99
    Thermo Fisher nano drop 2000 spectrometer
    Nano Drop 2000 Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano drop 2000 spectrometer/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nano drop 2000 spectrometer - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher nano drop 2000 2000c spectrometer
    Nano Drop 2000 2000c Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano drop 2000 2000c spectrometer/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nano drop 2000 2000c spectrometer - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher spectrometer
    Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spectrometer/product/Thermo Fisher
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    spectrometer - by Bioz Stars, 2020-03
    99/100 stars
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