Structured Review

Genmed atp synthase activity
Absence of AtpB subunit production and reduction of <t>ATP</t> <t>synthase</t> activity in rice osppr676 mutants. ( a ) Western blot analysis with AtpB antibody on total proteins from the osppr676-1 and osppr676-2 mutants and WT seedlings (Up panel) and Coomassie Blue staining of the loading amount of samples (Down panel). Full-length gel and blot with original contrast are displayed in Fig. S6 . ( b ) ATP synthase activity measured in the WT, osppr676-1 and osppr676-2 .
Atp Synthase Activity, supplied by Genmed, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atp synthase activity/product/Genmed
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
atp synthase activity - by Bioz Stars, 2020-04
92/100 stars

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1) Product Images from "A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice"

Article Title: A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice

Journal: Scientific Reports

doi: 10.1038/s41598-017-10727-x

Absence of AtpB subunit production and reduction of ATP synthase activity in rice osppr676 mutants. ( a ) Western blot analysis with AtpB antibody on total proteins from the osppr676-1 and osppr676-2 mutants and WT seedlings (Up panel) and Coomassie Blue staining of the loading amount of samples (Down panel). Full-length gel and blot with original contrast are displayed in Fig. S6 . ( b ) ATP synthase activity measured in the WT, osppr676-1 and osppr676-2 .
Figure Legend Snippet: Absence of AtpB subunit production and reduction of ATP synthase activity in rice osppr676 mutants. ( a ) Western blot analysis with AtpB antibody on total proteins from the osppr676-1 and osppr676-2 mutants and WT seedlings (Up panel) and Coomassie Blue staining of the loading amount of samples (Down panel). Full-length gel and blot with original contrast are displayed in Fig. S6 . ( b ) ATP synthase activity measured in the WT, osppr676-1 and osppr676-2 .

Techniques Used: Activity Assay, Western Blot, Staining

Related Articles

Transfection:

Article Title: Depletion of C3orf1/TIMMDC1 Inhibits Migration and Proliferation in 95D Lung Carcinoma Cells
Article Snippet: Ten micrograms of mitochondria were used to detect ATP synthase activity by using the mitochondrial complex V activity quantitative analysis kit (Genmed Scientifics, Cat. GMS50083). .. The ATPase activity of 95D cells transfected with control or c3orf1 siRNA was obtained by measuring absorbance at 340 nm.

Luciferase:

Article Title: Natural variation in CTB4a enhances rice adaptation to cold habitats
Article Snippet: ATP synthase activity was determined by the protocol provided with the ATPase Activity Assay Kit (GenMed Scientifics, GMS50248.3). .. ATP was extracted in lysis buffer (50 mM Tris–HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 0.2% TritonX-100 and 2% glycerol) and quantified based on the requirement of luciferase for ATP in producing light following the manufacturer's protocol supplied with the ATP Determination Kit (Invitrogen, A22066).

Nadh Coupled Enzyme Assay:

Article Title: A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice
Article Snippet: .. Measurement of ATP synthase activity The protein were prepared from 200 mg fresh leaf sample and measured by continuous NADH-coupled enzyme assay kit (Genmed 50250.5, Shanghai, China). ..

Isolation:

Article Title: Depletion of C3orf1/TIMMDC1 Inhibits Migration and Proliferation in 95D Lung Carcinoma Cells
Article Snippet: Mitochondria of 95D cells were isolated using the animal cells/tissues mitochondria extraction kit (Genmed Scientifics, Shanghai, China; Cat. GSM10006). .. Ten micrograms of mitochondria were used to detect ATP synthase activity by using the mitochondrial complex V activity quantitative analysis kit (Genmed Scientifics, Cat. GMS50083).

Article Title: Parkin Protects against Oxygen-Glucose Deprivation/Reperfusion Insult by Promoting Drp1 Degradation
Article Snippet: Mitochondria of N2a cells were isolated using the animal cells/tissues mitochondria extraction kit (Genmed Scientifics, Cat. GSM10006). .. Ten micrograms of mitochondria was used to detect ATP synthase activity by using the mitochondrial complex V activity quantitative analysis kit (Genmed Scientifics, Cat. GMS50083).

Incubation:

Article Title: Depletion of C3orf1/TIMMDC1 Inhibits Migration and Proliferation in 95D Lung Carcinoma Cells
Article Snippet: For mitochondrial potential detection, cells were incubated in a TMRM (40 nM) solution for 15–20 min at 37 °C in the dark. .. Ten micrograms of mitochondria were used to detect ATP synthase activity by using the mitochondrial complex V activity quantitative analysis kit (Genmed Scientifics, Cat. GMS50083).

Spectrophotometry:

Article Title: A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice
Article Snippet: Measurement of ATP synthase activity The protein were prepared from 200 mg fresh leaf sample and measured by continuous NADH-coupled enzyme assay kit (Genmed 50250.5, Shanghai, China). .. Absorbance data are collected at A340 using a SPECTRAmax 250 microplate spectrophotometer equipped with SOFTmax PRO software (Molecular Devices).

Generated:

Article Title: Parkin Protects against Oxygen-Glucose Deprivation/Reperfusion Insult by Promoting Drp1 Degradation
Article Snippet: Ten micrograms of mitochondria was used to detect ATP synthase activity by using the mitochondrial complex V activity quantitative analysis kit (Genmed Scientifics, Cat. GMS50083). .. Briefly, the ADP generated by complex V reacts with phosphoenolpyruvic acid (1 mM) to pyruvate in the presence of pyruvate kinase (1.5 U/mL).

Activity Assay:

Article Title: Depletion of C3orf1/TIMMDC1 Inhibits Migration and Proliferation in 95D Lung Carcinoma Cells
Article Snippet: .. Ten micrograms of mitochondria were used to detect ATP synthase activity by using the mitochondrial complex V activity quantitative analysis kit (Genmed Scientifics, Cat. GMS50083). .. The ATPase activity of 95D cells transfected with control or c3orf1 siRNA was obtained by measuring absorbance at 340 nm.

Article Title: A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice
Article Snippet: .. Measurement of ATP synthase activity The protein were prepared from 200 mg fresh leaf sample and measured by continuous NADH-coupled enzyme assay kit (Genmed 50250.5, Shanghai, China). ..

Article Title: Natural variation in CTB4a enhances rice adaptation to cold habitats
Article Snippet: .. ATP synthase activity was determined by the protocol provided with the ATPase Activity Assay Kit (GenMed Scientifics, GMS50248.3). .. ATP was extracted in lysis buffer (50 mM Tris–HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 0.2% TritonX-100 and 2% glycerol) and quantified based on the requirement of luciferase for ATP in producing light following the manufacturer's protocol supplied with the ATP Determination Kit (Invitrogen, A22066).

Article Title: Parkin Protects against Oxygen-Glucose Deprivation/Reperfusion Insult by Promoting Drp1 Degradation
Article Snippet: .. Ten micrograms of mitochondria was used to detect ATP synthase activity by using the mitochondrial complex V activity quantitative analysis kit (Genmed Scientifics, Cat. GMS50083). .. Briefly, the ADP generated by complex V reacts with phosphoenolpyruvic acid (1 mM) to pyruvate in the presence of pyruvate kinase (1.5 U/mL).

Lysis:

Article Title: Natural variation in CTB4a enhances rice adaptation to cold habitats
Article Snippet: ATP synthase activity was determined by the protocol provided with the ATPase Activity Assay Kit (GenMed Scientifics, GMS50248.3). .. ATP was extracted in lysis buffer (50 mM Tris–HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 0.2% TritonX-100 and 2% glycerol) and quantified based on the requirement of luciferase for ATP in producing light following the manufacturer's protocol supplied with the ATP Determination Kit (Invitrogen, A22066).

Software:

Article Title: A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice
Article Snippet: Measurement of ATP synthase activity The protein were prepared from 200 mg fresh leaf sample and measured by continuous NADH-coupled enzyme assay kit (Genmed 50250.5, Shanghai, China). .. Absorbance data are collected at A340 using a SPECTRAmax 250 microplate spectrophotometer equipped with SOFTmax PRO software (Molecular Devices).

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    Genmed continuous nadh coupled enzyme assay kit
    Continuous Nadh Coupled Enzyme Assay Kit, supplied by Genmed, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/continuous nadh coupled enzyme assay kit/product/Genmed
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    continuous nadh coupled enzyme assay kit - by Bioz Stars, 2020-04
    92/100 stars
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