nadh coupled colorimetric assay  (Millipore)


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    Structured Review

    Millipore nadh coupled colorimetric assay
    Nadh Coupled Colorimetric Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nadh coupled colorimetric assay/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nadh coupled colorimetric assay - by Bioz Stars, 2020-04
    99/100 stars

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    Centrifugation:

    Article Title: Polyglutamine toxicity in yeast induces metabolic alterations and mitochondrial defects
    Article Snippet: NAD + /NADH levels were determined using a commercial available NAD/NADH Quantification Kit (Sigma-Aldrich, St. Louis, MO, USA). .. Cell debris was removed by centrifugation (17000 g, 4 °C) and the lysate further treated according to the protocol.

    Filtration:

    Article Title: Glycerol-3-Phosphate Shuttle Is Involved in Development and Virulence in the Rice Blast Fungus Pyricularia oryzae
    Article Snippet: Intracellular NAD+ and NADH were quantified using the NAD/NADH quantification kit according to the technical bulletin (Sigma-Aldrich, United States). .. Samples were centrifuged at 12,000 × g for 5 min and the supernatants were then deproteinized by filtration through a 10 kDa cut-off spin filter (7,500 × g for 7 min at 4°C).

    Proton NMR:

    Article Title: Multiple turnovers of the nicotino-enzyme PdxB require ?-keto acids as co-substrates
    Article Snippet: Lysozyme, NAD+ , NADH, guanidine HCl, urea, 2,6-dichloroindole phenol (DCIP), N-nitrosodimethylamine (NDMA), methylene blue, potassium ferricyanide, pyruvate, OAA, αKG, D-2-hydroxyglutarate, and L-2-hydroxyglutarate were obtained from Sigma-Aldrich. .. 4PE was synthesized by Yehor Novikov (procedure to be published elsewhere) and was characterized by 1 H-NMR (500 MHz, D2 O): d 4.08 (1H, d, J = 5.0 Hz), 3.97 (1H, m), 3.84 (1 H, m), 3.78 (1H, m). αKG was characterized by 1 H-NMR (500 MHz, D2 O): 2.96 (2H, t), 2.39 (2H, t).

    Synthesized:

    Article Title: Multiple turnovers of the nicotino-enzyme PdxB require ?-keto acids as co-substrates
    Article Snippet: Lysozyme, NAD+ , NADH, guanidine HCl, urea, 2,6-dichloroindole phenol (DCIP), N-nitrosodimethylamine (NDMA), methylene blue, potassium ferricyanide, pyruvate, OAA, αKG, D-2-hydroxyglutarate, and L-2-hydroxyglutarate were obtained from Sigma-Aldrich. .. 4PE was synthesized by Yehor Novikov (procedure to be published elsewhere) and was characterized by 1 H-NMR (500 MHz, D2 O): d 4.08 (1H, d, J = 5.0 Hz), 3.97 (1H, m), 3.84 (1 H, m), 3.78 (1H, m). αKG was characterized by 1 H-NMR (500 MHz, D2 O): 2.96 (2H, t), 2.39 (2H, t).

    Enzyme Activity Assay:

    Article Title: Biochemical and structural investigations on phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis
    Article Snippet: Paragraph title: Enzyme activity assay ... The 200μl reaction mixture contained 50mM potassium phosphate buffer pH 7.5, 10mM Mg2 Cl, 10mM KCl, 2mM ATP, 2mM R5P, 1.5mM PEP, 0.2mM NADH, 5U myokinase, 20U pyruvate kinase, and 10U lactate dehydrogenase (all the components were purchased from Sigma Aldrich).

    Incubation:

    Article Title: Glycerol-3-Phosphate Shuttle Is Involved in Development and Virulence in the Rice Blast Fungus Pyricularia oryzae
    Article Snippet: Intracellular NAD+ and NADH were quantified using the NAD/NADH quantification kit according to the technical bulletin (Sigma-Aldrich, United States). .. 20 mg of mycelium samples were ground in liquid nitrogen, transferred to 1.5 ml tubes which contained 400 μl of NADH/NAD extraction buffer, and incubated 10 min at room temperature.

    Article Title: Biochemical and structural investigations on phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis
    Article Snippet: The 200μl reaction mixture contained 50mM potassium phosphate buffer pH 7.5, 10mM Mg2 Cl, 10mM KCl, 2mM ATP, 2mM R5P, 1.5mM PEP, 0.2mM NADH, 5U myokinase, 20U pyruvate kinase, and 10U lactate dehydrogenase (all the components were purchased from Sigma Aldrich). .. The enzyme activation by Mg2+ v = V max 1 + K m S + S K i (1) in which v, Vmax , S, and Km represent, respectively, steady state reaction rate (μmol min-1 mg-1 ), maximum reaction rate, substrate concentration, and Michaelis-Menten constant for S. The enzymatic reaction was started by the addition of MsPrsA after 1 minute of incubation, and followed by the decrease in the absorbance at 340nm of NADH (6220 M-1 cm-1 ) at 25°C.

    Activity Assay:

    Article Title: Crystal structures and kinetic properties of enoyl-acyl carrier protein reductase I from Candidatus Liberibacter asiaticus
    Article Snippet: .. The catalytic activity of ClFabI that reduced enoyl–ACP coupled with the concomitant oxidation of NADH was assayed based on a modified protocol., INH, NADH, crotonoyl-CoA, and dimethyl sulfoxide (DMSO) were purchased from Sigma. .. All experiments were performed on a Spectra Max M2 Molecular Devices at 28°C.

    Article Title: Structure and catalytic mechanism of monodehydroascorbate reductase, MDHAR, from Oryza sativa L. japonica
    Article Snippet: Paragraph title: Enzymatic activity ... The assay was performed at 25 °C with a reaction mixture (1 mL) containing 50 mM potassium phosphate, pH 7.2, 0.25 mM NADH or NADPH, 2 mM AsA, 1 unit AsA oxidase (Sigma-Aldrich, USA), and purified protein (10 μg).

    Article Title: Biochemical and structural investigations on phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis
    Article Snippet: The assay indirectly quantifies the amount of AMP produced as a product of the MsPrsA activity using an NADH-coupled enzyme system. .. The 200μl reaction mixture contained 50mM potassium phosphate buffer pH 7.5, 10mM Mg2 Cl, 10mM KCl, 2mM ATP, 2mM R5P, 1.5mM PEP, 0.2mM NADH, 5U myokinase, 20U pyruvate kinase, and 10U lactate dehydrogenase (all the components were purchased from Sigma Aldrich).

    Article Title: Ralstonia solanacearum RSp0194 Encodes a Novel 3-Keto-Acyl Carrier Protein Synthase III
    Article Snippet: .. Materials Malonyl coenzyme A (malonyl-CoA), acyl-CoAs, fatty acids, NADH, NADPH, and antibiotics were purchased from Sigma-Aldrich (St. Louis, MO, USA); Takara Biotechnology Co., Ltd. (Dalian, China) provided molecular biology reagents; the Ni2+ -agarose columns were purchased from Invitrogen (Shanghai, China); American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA) provided sodium [1-14 C] acetate (specific activity, 50 mCi/mM); and Bio-Rad (Hercules, CA, USA) provided the Quick Start Bradford dye reagent. ..

    Expressing:

    Article Title: Multiple turnovers of the nicotino-enzyme PdxB require ?-keto acids as co-substrates
    Article Snippet: Bugbuster, benzonase, and the expression plasmids pET21b and pET45b were obtained from EMD Biosciences. .. Lysozyme, NAD+ , NADH, guanidine HCl, urea, 2,6-dichloroindole phenol (DCIP), N-nitrosodimethylamine (NDMA), methylene blue, potassium ferricyanide, pyruvate, OAA, αKG, D-2-hydroxyglutarate, and L-2-hydroxyglutarate were obtained from Sigma-Aldrich.

    Modification:

    Article Title: Crystal structures and kinetic properties of enoyl-acyl carrier protein reductase I from Candidatus Liberibacter asiaticus
    Article Snippet: .. The catalytic activity of ClFabI that reduced enoyl–ACP coupled with the concomitant oxidation of NADH was assayed based on a modified protocol., INH, NADH, crotonoyl-CoA, and dimethyl sulfoxide (DMSO) were purchased from Sigma. .. All experiments were performed on a Spectra Max M2 Molecular Devices at 28°C.

    High Performance Liquid Chromatography:

    Article Title: High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli
    Article Snippet: To determine succinate, pyruvate, acetate and lactate concentrations, culture samples were centrifuged at 12,000g for 5 min and the aqueous supernatant used for HPLC analysis on an Agilent 1100 Series HPLC system equipped with a cation exchange column. (Aminex HPX87-H, Bio-Rad, Hercules, CA, USA), a UV absorbance detector (Agilent Technologies, G1315D) and a refractive index (RI) detector (Agilent Technologies, HP1047A). .. The intracellular NADH and NAD+ levels were determined by NAD/NADH Quantitation Kit (SIGMA) [ ].

    Article Title: Oligo-carrageenan kappa increases NADPH, ascorbate and glutathione syntheses and TRR/TRX activities enhancing photosynthesis, basal metabolism, and growth in Eucalyptus trees
    Article Snippet: Pyridine nucleotides were analyzed by High Performance Liquid Chromatography (HPLC) using an Agilent 1260 Infinity system and data was compiled using OpenLAB software. .. Pure NADPH, NADH, NAD, and NADP (Sigma St. Louis, USA) were dissolved in filtered water and used as standards.

    Flow Cytometry:

    Article Title: High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli
    Article Snippet: A mobile phase of 5 mM H2 SO4 solution at a 0.4 mL/min flow rate was used. .. The intracellular NADH and NAD+ levels were determined by NAD/NADH Quantitation Kit (SIGMA) [ ].

    Article Title: Oligo-carrageenan kappa increases NADPH, ascorbate and glutathione syntheses and TRR/TRX activities enhancing photosynthesis, basal metabolism, and growth in Eucalyptus trees
    Article Snippet: Pyridine nucleotides (20 μL) were separated on a reversed-phase C-18 column (5 μm particle size, 4.6 mm inner diameter, 15 cm length) at 22°C, eluted using solvent A (50 mM ammonium acetate pH 6.9) and solvent B (100% acetonitrile) with linear step gradients of 25 min from 0 to 5% of solvent B, 7 min from 5 to 95% of solvent B, 3 min from 95 to 100% of solvent B and 10 min of 0% solvent B using a flow rate of 1 mL min−1 . .. Pure NADPH, NADH, NAD, and NADP (Sigma St. Louis, USA) were dissolved in filtered water and used as standards.

    Gas Chromatography:

    Article Title: Impact of maternal dietary fat supplementation during gestation upon skeletal muscle in neonatal pigs
    Article Snippet: NAD+/NADH ratio was determined using a colorimetric commercial kit (Sigma-Aldrich Co LLC, Gillingham, UK) and normalised to the quantity of tissue dissected i.e. moles per gram of protein. .. Phospholipid composition was determined by gas-chromatography in which the chloroform phase was evaporated by applying a nitrogen stream and then 2 ml of hexane was added to each sample.

    Activation Assay:

    Article Title: Biochemical and structural investigations on phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis
    Article Snippet: The 200μl reaction mixture contained 50mM potassium phosphate buffer pH 7.5, 10mM Mg2 Cl, 10mM KCl, 2mM ATP, 2mM R5P, 1.5mM PEP, 0.2mM NADH, 5U myokinase, 20U pyruvate kinase, and 10U lactate dehydrogenase (all the components were purchased from Sigma Aldrich). .. The enzyme activation by Mg2+ v = V max 1 + K m S + S K i (1) in which v, Vmax , S, and Km represent, respectively, steady state reaction rate (μmol min-1 mg-1 ), maximum reaction rate, substrate concentration, and Michaelis-Menten constant for S. The enzymatic reaction was started by the addition of MsPrsA after 1 minute of incubation, and followed by the decrease in the absorbance at 340nm of NADH (6220 M-1 cm-1 ) at 25°C.

    Cell Culture:

    Article Title: Glycerol-3-Phosphate Shuttle Is Involved in Development and Virulence in the Rice Blast Fungus Pyricularia oryzae
    Article Snippet: For strains cultured under a light–dark cycle, aerial mycelium samples were collected in the daytime. .. Intracellular NAD+ and NADH were quantified using the NAD/NADH quantification kit according to the technical bulletin (Sigma-Aldrich, United States).

    Inhibition:

    Article Title: Crystal structures and kinetic properties of enoyl-acyl carrier protein reductase I from Candidatus Liberibacter asiaticus
    Article Snippet: Paragraph title: ClFabI inhibition assays ... The catalytic activity of ClFabI that reduced enoyl–ACP coupled with the concomitant oxidation of NADH was assayed based on a modified protocol., INH, NADH, crotonoyl-CoA, and dimethyl sulfoxide (DMSO) were purchased from Sigma.

    Nad NADH Assay:

    Article Title: Pyruvate Treatment Restores the Effectiveness of Chemotherapeutic Agents in Human Colon Adenocarcinoma and Pleural Mesothelioma Cells
    Article Snippet: Paragraph title: 4.13. NAD+ /NADH Assay ... NAD+ and NADH were measured using the NAD+ /NADH Quantification Colorimetric Kit from Sigma-Aldrich (Milan, Italy), which allows determination of NAD+ , NADH and their ratio, without the requirement to purification steps.

    Fluorescence:

    Article Title: Oligo-carrageenan kappa increases NADPH, ascorbate and glutathione syntheses and TRR/TRX activities enhancing photosynthesis, basal metabolism, and growth in Eucalyptus trees
    Article Snippet: NAD and NADP were detected by absorbance at 260 nm using a diode array detector and NADH and NADPH using an excitation wavelength of 340 nm and emission wavelength of 480 nm, using a fluorescence detector. .. Pure NADPH, NADH, NAD, and NADP (Sigma St. Louis, USA) were dissolved in filtered water and used as standards.

    Methylation:

    Article Title: Impact of maternal dietary fat supplementation during gestation upon skeletal muscle in neonatal pigs
    Article Snippet: NAD+/NADH ratio was determined using a colorimetric commercial kit (Sigma-Aldrich Co LLC, Gillingham, UK) and normalised to the quantity of tissue dissected i.e. moles per gram of protein. .. The phospholipids were transmethylated by adding 40 μl of methyl acetate and 40 μl of methylation reagent (30% sodium methoxide, 4.1 ml methanol; Fisher Scientific Ltd. Loughborough, UK) to each reaction and left to react for 10 minutes at room temperature.

    Purification:

    Article Title: Structure and catalytic mechanism of monodehydroascorbate reductase, MDHAR, from Oryza sativa L. japonica
    Article Snippet: .. The assay was performed at 25 °C with a reaction mixture (1 mL) containing 50 mM potassium phosphate, pH 7.2, 0.25 mM NADH or NADPH, 2 mM AsA, 1 unit AsA oxidase (Sigma-Aldrich, USA), and purified protein (10 μg). .. The absorbance was measured at 340 nm by monitoring NAD(P)H oxidation, and the activity was calculated using an absorbance coefficient of 6.2 mM−1 cm−1 .

    Article Title: Pyruvate Treatment Restores the Effectiveness of Chemotherapeutic Agents in Human Colon Adenocarcinoma and Pleural Mesothelioma Cells
    Article Snippet: .. NAD+ and NADH were measured using the NAD+ /NADH Quantification Colorimetric Kit from Sigma-Aldrich (Milan, Italy), which allows determination of NAD+ , NADH and their ratio, without the requirement to purification steps. .. The intracellular NAD+ /NADH assay was performed according to the manufacturer’s specifications; NAD+ and NADH levels were adjusted for protein contents.

    Software:

    Article Title: Oligo-carrageenan kappa increases NADPH, ascorbate and glutathione syntheses and TRR/TRX activities enhancing photosynthesis, basal metabolism, and growth in Eucalyptus trees
    Article Snippet: Pyridine nucleotides were analyzed by High Performance Liquid Chromatography (HPLC) using an Agilent 1260 Infinity system and data was compiled using OpenLAB software. .. Pure NADPH, NADH, NAD, and NADP (Sigma St. Louis, USA) were dissolved in filtered water and used as standards.

    Quantitation Assay:

    Article Title: High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli
    Article Snippet: .. The intracellular NADH and NAD+ levels were determined by NAD/NADH Quantitation Kit (SIGMA) [ ]. .. Quantitative real-time reverse transcription (RT)-PCR analysis Escherichia coli ZTK and its derivatives were harvested when grown to the exponential phase under anaerobic condition.

    Spectrophotometry:

    Article Title: Biochemical and structural investigations on phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis
    Article Snippet: The 200μl reaction mixture contained 50mM potassium phosphate buffer pH 7.5, 10mM Mg2 Cl, 10mM KCl, 2mM ATP, 2mM R5P, 1.5mM PEP, 0.2mM NADH, 5U myokinase, 20U pyruvate kinase, and 10U lactate dehydrogenase (all the components were purchased from Sigma Aldrich). .. The assay was performed in quartz cuvettes using a Varian Cary 50-BiO UV-visible spectrophotometer equipped with a temperature-controlled cuvette holder.

    Article Title: High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli
    Article Snippet: Analytical techniques Cell growth was monitored by measuring the OD600 with an ultraviolet spectrophotometer (Beijing Puxi Universal Co. Ltd). .. The intracellular NADH and NAD+ levels were determined by NAD/NADH Quantitation Kit (SIGMA) [ ].

    Produced:

    Article Title: Biochemical and structural investigations on phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis
    Article Snippet: The assay indirectly quantifies the amount of AMP produced as a product of the MsPrsA activity using an NADH-coupled enzyme system. .. The 200μl reaction mixture contained 50mM potassium phosphate buffer pH 7.5, 10mM Mg2 Cl, 10mM KCl, 2mM ATP, 2mM R5P, 1.5mM PEP, 0.2mM NADH, 5U myokinase, 20U pyruvate kinase, and 10U lactate dehydrogenase (all the components were purchased from Sigma Aldrich).

    Concentration Assay:

    Article Title: Crystal structures and kinetic properties of enoyl-acyl carrier protein reductase I from Candidatus Liberibacter asiaticus
    Article Snippet: The catalytic activity of ClFabI that reduced enoyl–ACP coupled with the concomitant oxidation of NADH was assayed based on a modified protocol., INH, NADH, crotonoyl-CoA, and dimethyl sulfoxide (DMSO) were purchased from Sigma. .. During the course of the analysis of ClFabI, it is possible for an increase in the concentration of NAD+ due to the oxidation of NADH, which is the coenzyme of ClFabI.

    Article Title: Biochemical and structural investigations on phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis
    Article Snippet: The 200μl reaction mixture contained 50mM potassium phosphate buffer pH 7.5, 10mM Mg2 Cl, 10mM KCl, 2mM ATP, 2mM R5P, 1.5mM PEP, 0.2mM NADH, 5U myokinase, 20U pyruvate kinase, and 10U lactate dehydrogenase (all the components were purchased from Sigma Aldrich). .. The enzyme activation by Mg2+ v = V max 1 + K m S + S K i (1) in which v, Vmax , S, and Km represent, respectively, steady state reaction rate (μmol min-1 mg-1 ), maximum reaction rate, substrate concentration, and Michaelis-Menten constant for S. The enzymatic reaction was started by the addition of MsPrsA after 1 minute of incubation, and followed by the decrease in the absorbance at 340nm of NADH (6220 M-1 cm-1 ) at 25°C.

    Article Title: High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli
    Article Snippet: The biomass concentration was calculated from OD600 values using an experimentally determined correlation with 1 OD600 unit being equal to 0.38 g/L cell dry weight (CDW) [ ]. .. The intracellular NADH and NAD+ levels were determined by NAD/NADH Quantitation Kit (SIGMA) [ ].

    other:

    Article Title: A Differential Redox Regulation of the Pathways Metabolizing Glyceraldehyde-3-Phosphate Tunes the Production of Reducing Power in the Cytosol of Plant Cells
    Article Snippet: Chemicals Rabbit muscle aldolase, NAD+ , NADP+ , NADH, NADPH, Fru1,6bisP, 3PGA, diamide, H2 O2 , sodium nitroprusside (SNP), and DTT were purchased from Sigma-Aldrich (St. Louis, MO, USA).

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    Millipore histone deacetylases
    Histone Deacetylases, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/histone deacetylases/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    histone deacetylases - by Bioz Stars, 2020-04
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